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Environmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting.

Abstract A Q fever outbreak was declared in February 2016 in a company that manufactures hoists and chains and therefore with no apparent occupational-associated risk. Coxiella burnetii infection was diagnosed by serology in eight of the 29 workers of the company; seven of them had fever or flu-like signs and five had pneumonia, one requiring hospitalisation. A further case of C. burnetii pneumonia was diagnosed in a local resident. Real-time PCR (RTi-PCR) showed a widespread distribution of C. burnetii DNA in dust samples collected from the plant facilities, thus confirming the exposure of workers to the infection inside the factory. Epidemiological investigations identified a goat flock with high C. burnetii seroprevalence and active shedding which was owned and managed by one of the workers of the company as possible source of infection. Genotyping by multispacer sequence typing (MST) and a 10-loci single-nucleotide polymorphism (SNP) discrimination using RTi-PCR identified the same genotype (MST18 and SNP type 8, respectively) in the farm and the factory. These results confirmed the link between the goat farm and the outbreak and allowed the identification of the source of infection. The circumstances and possible vehicles for the bacteria entering the factory are discussed.
PMID
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Authors

Mayor MeshTerms
Keywords

Coxiella burnetii

Q fever

dust

genotyping

outbreak

Journal Title epidemiology and infection
Publication Year Start




PMID- 28434420
OWN - NLM
STAT- Publisher
DA  - 20170424
LR  - 20170424
IS  - 1469-4409 (Electronic)
IS  - 0950-2688 (Linking)
DP  - 2017 Apr 24
TI  - Environmental sampling coupled with real-time PCR and genotyping to investigate
      the source of a Q fever outbreak in a work setting.
PG  - 1-9
LID - 10.1017/S0950268817000796 [doi]
AB  - A Q fever outbreak was declared in February 2016 in a company that manufactures
      hoists and chains and therefore with no apparent occupational-associated risk.
      Coxiella burnetii infection was diagnosed by serology in eight of the 29 workers 
      of the company; seven of them had fever or flu-like signs and five had pneumonia,
      one requiring hospitalisation. A further case of C. burnetii pneumonia was
      diagnosed in a local resident. Real-time PCR (RTi-PCR) showed a widespread
      distribution of C. burnetii DNA in dust samples collected from the plant
      facilities, thus confirming the exposure of workers to the infection inside the
      factory. Epidemiological investigations identified a goat flock with high C.
      burnetii seroprevalence and active shedding which was owned and managed by one of
      the workers of the company as possible source of infection. Genotyping by
      multispacer sequence typing (MST) and a 10-loci single-nucleotide polymorphism
      (SNP) discrimination using RTi-PCR identified the same genotype (MST18 and SNP
      type 8, respectively) in the farm and the factory. These results confirmed the
      link between the goat farm and the outbreak and allowed the identification of the
      source of infection. The circumstances and possible vehicles for the bacteria
      entering the factory are discussed.
FAU - Hurtado, A
AU  - Hurtado A
AD  - Department of Animal Health,NEIKER - Instituto Vasco de Investigacion y
      Desarrollo Agrario,Derio,Bizkaia,Spain.
FAU - Alonso, E
AU  - Alonso E
AD  - Department of Epidemiology,Subdireccion de Salud Publica de Bizkaia,Gobierno
      Vasco,Bilbao,Bizkaia,Spain.
FAU - Aspiritxaga, I
AU  - Aspiritxaga I
AD  - Department of Epidemiology,Subdireccion de Salud Publica de Bizkaia,Gobierno
      Vasco,Bilbao,Bizkaia,Spain.
FAU - Lopez Etxaniz, I
AU  - Lopez Etxaniz I
AD  - Department of Epidemiology,OSALAN - Instituto Vasco de Seguridad y Salud
      Laborales,Barakaldo,Bizkaia,Spain.
FAU - Ocabo, B
AU  - Ocabo B
AD  - Department of Agriculture,Diputacion Foral de Bizkaia,Bilbao,Bizkaia,Spain.
FAU - Barandika, J F
AU  - Barandika JF
AD  - Department of Animal Health,NEIKER - Instituto Vasco de Investigacion y
      Desarrollo Agrario,Derio,Bizkaia,Spain.
FAU - Fernandez-Ortiz DE Murua, J I
AU  - Fernandez-Ortiz DE Murua JI
AD  - Department of Agriculture,Diputacion Foral de Araba,Vitoria-Gasteiz,Spain.
FAU - Urbaneja, F
AU  - Urbaneja F
AD  - Department of Epidemiology,OSALAN - Instituto Vasco de Seguridad y Salud
      Laborales,Barakaldo,Bizkaia,Spain.
FAU - Alvarez-Alonso, R
AU  - Alvarez-Alonso R
AD  - Department of Animal Health,NEIKER - Instituto Vasco de Investigacion y
      Desarrollo Agrario,Derio,Bizkaia,Spain.
FAU - Jado, I
AU  - Jado I
AD  - Instituto de Salud Carlos III,Majadahonda,Madrid,Spain.
FAU - Garcia-Perez, A L
AU  - Garcia-Perez AL
AD  - Department of Animal Health,NEIKER - Instituto Vasco de Investigacion y
      Desarrollo Agrario,Derio,Bizkaia,Spain.
LA  - eng
PT  - Journal Article
DEP - 20170424
PL  - England
TA  - Epidemiol Infect
JT  - Epidemiology and infection
JID - 8703737
OTO - NOTNLM
OT  - Coxiella burnetii
OT  - Q fever
OT  - dust
OT  - genotyping
OT  - outbreak
EDAT- 2017/04/25 06:00
MHDA- 2017/04/25 06:00
CRDT- 2017/04/25 06:00
AID - S0950268817000796 [pii]
AID - 10.1017/S0950268817000796 [doi]
PST - aheadofprint
SO  - Epidemiol Infect. 2017 Apr 24:1-9. doi: 10.1017/S0950268817000796.

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