PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Differentially expressed genes during spontaneous lytic switch of Marek's disease virus in lymphoblastoid cell lines determined by global gene expression profiling.

Abstract Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes Marek's disease and is characterized by visceral CD4+TCRαβ+ T-cell lymphomas in susceptible hosts. Immortal cell lines harbouring the viral genome have been generated from ex vivo cultures of MD tumours. As readily available sources of large numbers of cells, MDV-transformed lymphoblastoid cell lines (LCLs) are extremely valuable for studies of virus-host interaction. While the viral genome in most cells is held in a latent state, minor populations of cells display spontaneous reactivation identifiable by the expression of lytic viral genes. Spontaneous reactivation in these cells presents an opportunity to investigate the biological processes involved in the virus reactivation. For detailed characterization of the molecular events associated with reactivation, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express enhanced green fluorescent protein (EGFP) fused with the UL47. We used fluorescence-activated cell sorting to purify the low-frequency EGFP-positive cells with a spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-seq using Illumina HiSeq2500. Ingenuity pathway analysis on more than 2000 differentially expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations identified the biological pathways involved in the reactivation. Virus-reactivating cells exhibited differential expression of a significant number of viral genes, with hierarchical differences in expression levels. Downregulation of a number of host genes including those directly involved in T-cell activation, such as CD3, CD28, ICOS and phospholipase C, was also noticed in the LCL undergoing lytic switch.
PMID
Related Publications

Differential expression of microRNAs in Marek's disease virus-transformed T-lymphoma cell lines.

Recombinant Marek's disease virus (MDV)-derived lymphoblastoid cell lines: regulation of a marker gene within the context of the MDV genome.

Fluorescently tagged pUL47 of Marek's disease virus reveals differential tissue expression of the tegument protein in vivo.

Latency of Marek's disease virus (MDV) in a reticuloendotheliosis virus-transformed T-cell line. II: expression of the latent MDV genome.

Construction and characterization of Marek's disease viruses having green fluorescent protein expression tied directly or indirectly to phosphoprotein 38 expression.

Authors

Mayor MeshTerms
Keywords
Journal Title the journal of general virology
Publication Year Start




PMID- 28475033
OWN - NLM
STAT- In-Process
DA  - 20170505
LR  - 20170505
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 98
IP  - 4
DP  - 2017 Apr
TI  - Differentially expressed genes during spontaneous lytic switch of Marek's disease
      virus in lymphoblastoid cell lines determined by global gene expression
      profiling.
PG  - 779-790
LID - 10.1099/jgv.0.000744 [doi]
AB  - Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes Marek's
      disease and is characterized by visceral CD4+TCRalphabeta+ T-cell lymphomas in
      susceptible hosts. Immortal cell lines harbouring the viral genome have been
      generated from ex vivo cultures of MD tumours. As readily available sources of
      large numbers of cells, MDV-transformed lymphoblastoid cell lines (LCLs) are
      extremely valuable for studies of virus-host interaction. While the viral genome 
      in most cells is held in a latent state, minor populations of cells display
      spontaneous reactivation identifiable by the expression of lytic viral genes.
      Spontaneous reactivation in these cells presents an opportunity to investigate
      the biological processes involved in the virus reactivation. For detailed
      characterization of the molecular events associated with reactivation, we used
      two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a
      recombinant MDV engineered to express enhanced green fluorescent protein (EGFP)
      fused with the UL47. We used fluorescence-activated cell sorting to purify the
      low-frequency EGFP-positive cells with a spontaneously activating viral genome
      from the majority EGFP-negative cells and analysed their gene expression profiles
      by RNA-seq using Illumina HiSeq2500. Ingenuity pathway analysis on more than 2000
      differentially expressed genes between the lytically infected (EGFP-positive) and
      latently infected (EGFP-negative) cell populations identified the biological
      pathways involved in the reactivation. Virus-reactivating cells exhibited
      differential expression of a significant number of viral genes, with hierarchical
      differences in expression levels. Downregulation of a number of host genes
      including those directly involved in T-cell activation, such as CD3, CD28, ICOS
      and phospholipase C, was also noticed in the LCL undergoing lytic switch.
FAU - Mwangi, William N
AU  - Mwangi WN
AD  - 1Avian Viral Diseases Programme, UK-China Centre of Excellence on Avian Disease
      Research, The Pirbright Institute, Pirbright, Surrey, UK.
FAU - Vasoya, Deepali
AU  - Vasoya D
AD  - 2Division of Genetics and Genomics, The Roslin Institute, R(D)SVS, University of 
      Edinburgh, Easter Bush, Midlothian, UK.
FAU - Kgosana, Lydia B
AU  - Kgosana LB
AD  - 1Avian Viral Diseases Programme, UK-China Centre of Excellence on Avian Disease
      Research, The Pirbright Institute, Pirbright, Surrey, UK.
FAU - Watson, Mick
AU  - Watson M
AD  - 2Division of Genetics and Genomics, The Roslin Institute, R(D)SVS, University of 
      Edinburgh, Easter Bush, Midlothian, UK.
FAU - Nair, Venugopal
AU  - Nair V
AD  - 1Avian Viral Diseases Programme, UK-China Centre of Excellence on Avian Disease
      Research, The Pirbright Institute, Pirbright, Surrey, UK.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
EDAT- 2017/05/06 06:00
MHDA- 2017/05/06 06:00
CRDT- 2017/05/06 06:00
AID - 10.1099/jgv.0.000744 [doi]
PST - ppublish
SO  - J Gen Virol. 2017 Apr;98(4):779-790. doi: 10.1099/jgv.0.000744.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>