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Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T Cells.

Abstract Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL(-1) and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL(-1). These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment.
PMID
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Authors

Mayor MeshTerms
Keywords

7-epiclusianone

MAC-T

Rheedia brasiliensis

Streptococcus spp.

bacterial activity

cytotoxicity

mastitis

Journal Title molecules (basel, switzerland)
Publication Year Start




PMID- 28513553
OWN - NLM
STAT- MEDLINE
DA  - 20170517
DCOM- 20170524
LR  - 20170524
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 22
IP  - 5
DP  - 2017 May 17
TI  - Antibacterial Activity of 7-Epiclusianone and Its Novel Copper Metal Complex on
      Streptococcus spp. Isolated from Bovine Mastitis and Their Cytotoxicity in MAC-T 
      Cells.
LID - E823 [pii]
LID - 10.3390/molecules22050823 [doi]
AB  - Mastitis is an inflammation of mammary gland parenchyma that adversely affects
      bovine health and dairy production worldwide despite significant efforts to
      eradicate it. The aim of this work was to characterize the antimicrobial activity
      of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis
      fruit, its complex with copper against Streptococcus spp. isolated from bovine
      mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells
      (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid
      with optical activity. Its vibrational spectrum in the infrared region showed
      absorption bands in the high-frequency region, as well as bands that can be
      attributed to the unconjugated and conjugated stretching of the free ligand. The 
      complex was anhydrous. One of the tested bacterial strains was not sensitive to
      the compounds, while the other three had MIC values of 7.8 microg mL-1 and
      minimum bactericidal concentration (MBC) values between 15.6 and 31.3 microg
      mL-1. These two compounds are bacteriostatic, did not cause damage to the cell
      wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion.
      The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu
      exhibited desirable antimicrobial properties and could potentially be used in
      bovine mastitis treatment.
FAU - de Barros, Mariana
AU  - de Barros M
AD  - Department of Veterinary, Universidade Federal de Vicosa, Vicosa 36570-900,
      Brazil. [email protected]
FAU - Perciano, Pedro Griffo
AU  - Perciano PG
FAU - Dos Santos, Marcelo Henrique
AU  - Dos Santos MH
AD  - Department of Chemistry, Universidade Federal de Vicosa, Vicosa 36570-900,
      Brazil. [email protected]
FAU - De Oliveira, Leandro Licursi
AU  - De Oliveira LL
AD  - Department of General Biology, Universidade Federal de Vicosa, Vicosa 36570-900, 
      Brazil. [email protected]
FAU - Costa, Ederson D'Martin
AU  - Costa ED
AD  - Institute of Chemistry, Universidade Federal de Alfenas, Alfenas 37130-000,
      Brazil. [email protected]
FAU - Moreira, Maria Aparecida Scatamburlo
AU  - Moreira MAS
AD  - Department of Veterinary, Universidade Federal de Vicosa, Vicosa 36570-900,
      Brazil. [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170517
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (7-epiclusianone)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Benzophenones)
RN  - 0 (Benzoquinones)
RN  - 0 (Coordination Complexes)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/*pharmacology/toxicity
MH  - Bacterial Adhesion
MH  - Benzophenones/chemistry/*pharmacology/toxicity
MH  - Benzoquinones/chemistry/*pharmacology/toxicity
MH  - Cattle
MH  - Coordination Complexes/chemistry/pharmacology/toxicity
MH  - Copper/chemistry
MH  - Female
MH  - Mammary Glands, Animal/*cytology/drug effects
MH  - Mastitis, Bovine/*microbiology
MH  - Microbial Sensitivity Tests
MH  - Streptococcal Infections/microbiology/veterinary
MH  - Streptococcus/*drug effects/isolation & purification/physiology
MH  - Streptococcus agalactiae/drug effects/isolation & purification/physiology
OTO - NOTNLM
OT  - 7-epiclusianone
OT  - MAC-T
OT  - Rheedia brasiliensis
OT  - Streptococcus spp.
OT  - bacterial activity
OT  - cytotoxicity
OT  - mastitis
COI - The authors declare no conflict of interest.
EDAT- 2017/05/18 06:00
MHDA- 2017/05/26 06:00
CRDT- 2017/05/18 06:00
PHST- 2017/03/23 [received]
PHST- 2017/05/04 [revised]
PHST- 2017/05/10 [accepted]
AID - molecules22050823 [pii]
AID - 10.3390/molecules22050823 [doi]
PST - epublish
SO  - Molecules. 2017 May 17;22(5). pii: E823. doi: 10.3390/molecules22050823.

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