PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




 
PMID- 28516859
OWN - NLM
STAT- MEDLINE
DA  - 20170518
DCOM- 20170614
LR  - 20170614
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 66
IP  - 5
DP  - 2017 May
TI  - Recombinant esterase from Corynebacterium pseudotuberculosis in DNA and subunit
      recombinant vaccines partially protects mice against challenge.
PG  - 635-642
LID - 10.1099/jmm.0.000477 [doi]
AB  - PURPOSE: We tested the efficacy of the esterase encoded by cp1002_RS09720 from
      Corynebacteriumpseudotuberculosis in recombinant subunit and DNA caseous
      lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA
      vaccine candidates by mature epitope density analysis. METHODOLOGY: Gene
      cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and
      pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the
      recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), 
      pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS as a negative
      control (G4). Immunization occurred in two doses intercalated by a 21 day
      interval. Twenty-one days after the last dose administration, animals were
      challenged with a virulent C. pseudotuberculosis MIC-6 strain. RESULTS: G1 showed
      high levels of IgG1 and IgG2a on days 21 and 42 post-immunization and a
      significant level of IFN-gamma (P<0.05), suggesting a Th1 response. The
      protection levels obtained were 58.3 and 16.6 % for G1 and G2, respectively.
      CONCLUSION: The subunit vaccine composed of the recombinant esterase rCP09720 and
      Al(OH)3 is a promising antigenic formulation for use against CLA.
FAU - Brum, Alexandre Antunes
AU  - Brum AA
AD  - 1Laboratorio de Biotecnologia Infecto-parasitaria, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Rezende, Andrea de Fatima Silva
AU  - Rezende AFS
AD  - 1Laboratorio de Biotecnologia Infecto-parasitaria, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Brilhante, Francisco Silvestre
AU  - Brilhante FS
AD  - 1Laboratorio de Biotecnologia Infecto-parasitaria, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Collares, Thais
AU  - Collares T
AD  - 1Laboratorio de Biotecnologia Infecto-parasitaria, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Begnine, Karine
AU  - Begnine K
AD  - 2Grupo de Pesquisa em Oncologia Celular e Molecular, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Seixas, Fabiana Kommling
AU  - Seixas FK
AD  - 2Grupo de Pesquisa em Oncologia Celular e Molecular, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Collares, Tiago Veiras
AU  - Collares TV
AD  - 2Grupo de Pesquisa em Oncologia Celular e Molecular, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Dellagostin, Odir Antonio
AU  - Dellagostin OA
AD  - 3Laboratorio de Biologia Molecular, Centro de Desenvolvimento Tecnologico,
      Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
FAU - Azevedo, Vasco
AU  - Azevedo V
AD  - 4Laboratorio de Genetica Celular e Molecular, Instituto de Ciencias Biologicas,
      UFMG, Belo Horizonte, MG 31270-901, Brazil.
FAU - Santos, Anderson
AU  - Santos A
AD  - 5Faculdade de Computacao, UFU, Uberlandia, MG 38400-902, Brazil.
FAU - Portela, Ricardo Wagner
AU  - Portela RW
AD  - 6Laboratorio de Imunologia e Biologia Molecular, Instituto de Ciencias da Saude, 
      UFBA, Salvador, BA 40110-100, Brazil.
FAU - Borsuk, Sibele
AU  - Borsuk S
AD  - 1Laboratorio de Biotecnologia Infecto-parasitaria, Centro de Desenvolvimento
      Tecnologico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170518
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Vaccines, Synthetic)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.1.- (Esterases)
SB  - IM
MH  - Adjuvants, Immunologic
MH  - Animals
MH  - Corynebacterium Infections/immunology/*prevention & control
MH  - Corynebacterium pseudotuberculosis/*enzymology/genetics/*immunology/pathogenicity
MH  - Cytokines/secretion
MH  - Esterases/administration & dosage/*genetics/immunology
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/immunology
MH  - Lymphadenitis/microbiology/*prevention & control
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Th1 Cells/immunology
MH  - Vaccination
MH  - Vaccines, DNA/administration & dosage/*immunology
MH  - Vaccines, Synthetic/immunology/isolation & purification
EDAT- 2017/05/19 06:00
MHDA- 2017/06/15 06:00
CRDT- 2017/05/19 06:00
AID - 10.1099/jmm.0.000477 [doi]
PST - ppublish
SO  - J Med Microbiol. 2017 May;66(5):635-642. doi: 10.1099/jmm.0.000477. Epub 2017 May
      18.

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