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Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.

Abstract Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA).
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title journal of medical microbiology
Publication Year Start




PMID- 28516860
OWN - NLM
STAT- MEDLINE
DA  - 20170518
DCOM- 20170614
LR  - 20170614
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 66
IP  - 5
DP  - 2017 May
TI  - Salmonella DIVA vaccine reduces disease, colonization and shedding due to
      virulent S. Typhimurium infection in swine.
PG  - 651-661
LID - 10.1099/jmm.0.000482 [doi]
AB  - PURPOSE: Non-host-adapted Salmonella serovars, including the common human
      food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are
      opportunistic pathogens that can colonize food-producing animals without causing 
      overt disease. Interventions against Salmonella are needed to enhance food
      safety, protect animal health and allow the differentiation of infected from
      vaccinated animals (DIVA). METHODOLOGY: An attenuated S. Typhimurium DIVA vaccine
      (BBS 866) was characterized for the protection of pigs following challenge with
      virulent S. Typhimurium. The porcine transcriptional response to BBS 866
      vaccination was evaluated. RNA-Seq analysis was used to compare gene expression
      between BBS 866 and its parent; phenotypic assays were performed to confirm
      transcriptional differences observed between the strains. RESULTS: Vaccination
      significantly reduced fever and interferon-gamma (IFNgamma) levels in swine
      challenged with virulent S. Typhimurium compared to mock-vaccinated pigs.
      Salmonella faecal shedding and gastrointestinal tissue colonization were
      significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to
      its parental S. Typhimurium strain demonstrated reduced expression of the genes
      involved in cellular invasion and bacterial motility; decreased invasion of
      porcine-derived IPEC-J2 cells and swimming motility for the vaccine strain was
      consistent with the RNA-Seq analysis. Numerous membrane proteins were
      differentially expressed, which was an anticipated gene expression pattern due to
      the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq 
      analysis indicated that genes involved in the porcine immune and inflammatory
      response were differentially regulated at 2 days post-vaccination compared to
      pre-vaccination. CONCLUSION: Evaluation of the S. Typhimurium DIVA vaccine
      indicates that vaccination will provide both swine health and food safety
      benefits.
FAU - Bearson, Bradley L
AU  - Bearson BL
AD  - 1USDA/ARS/National Laboratory for Agriculture and the Environment, Ames, IA,
      50011, USA.
FAU - Bearson, Shawn M D
AU  - Bearson SMD
AD  - 2USDA/ARS/National Animal Disease Center, Ames, IA, 50010, USA.
FAU - Brunelle, Brian W
AU  - Brunelle BW
AD  - 2USDA/ARS/National Animal Disease Center, Ames, IA, 50010, USA.
FAU - Bayles, Darrell O
AU  - Bayles DO
AD  - 2USDA/ARS/National Animal Disease Center, Ames, IA, 50010, USA.
FAU - Lee, In Soo
AU  - Lee IS
AD  - 3Hannam University, Daejeon, Republic of Korea.
FAU - Kich, Jalusa D
AU  - Kich JD
AD  - 4Embrapa Swine and Poultry, Concordia, SC, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170518
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Salmonella Vaccines)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, DNA)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Bacterial Shedding
MH  - Cell Line
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Feces/microbiology
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Salmonella Infections, Animal/immunology/microbiology/*prevention & control
MH  - Salmonella Vaccines/*administration & dosage/immunology
MH  - Salmonella typhimurium/*immunology/metabolism/pathogenicity
MH  - Swine
MH  - Swine Diseases/microbiology/*prevention & control
MH  - Vaccination
MH  - Vaccines, Attenuated/administration & dosage/immunology
MH  - Vaccines, DNA/*administration & dosage/immunology
EDAT- 2017/05/19 06:00
MHDA- 2017/06/15 06:00
CRDT- 2017/05/19 06:00
AID - 10.1099/jmm.0.000482 [doi]
PST - ppublish
SO  - J Med Microbiol. 2017 May;66(5):651-661. doi: 10.1099/jmm.0.000482. Epub 2017 May
      18.

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