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Establishment of 5-Fluorouracil-resistant canine mammary tumor cell line.

Abstract Canine mammary tumors are the most common neoplasms in intact female dogs. The surgery cannot always solve the problem, chemotherapy are recommend to these patients. However, chemotherapy could always fail because of multidrug resistance (MDR). Through stepwise increasing 5-Fluorouracil (5-FU) concentration in the culture medium, a 5-FU-resistant canine mammary tumor cell line CMT7364/5-FU was established to disclose the molecular mechanism of the drug resistance. Cell morphology, cell sensitivity to drugs, growth curves, expression of proteins, and chemo-sensitivity in vivo were compared between the parental cell line and resistant cell line. As compared it to its parental cell line (CMT7364), CMT7364/5-FU showed different morphology, cross-resistant to other chemo-drugs and a prolonged population doubling time (PDT). The drug efflux pump proteins (ABCB1 and ABCG2) in CMT7364/5-FU were up-regulated. In vivo, the similar result revealed that CMT7364/5-FU cell line was more resistant to 5-FU. In conclusion, a 5-FU-resistant canine mammary tumor cell line (CMT7364/5-FU) was successfully established, it can serve as a good model for researching the mechanism of MDR and screening effective agents to reverse drug resistance.
PMID
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Authors

Mayor MeshTerms

Drug Resistance, Neoplasm

Keywords

5-Fluorouracil

ABC proteins

canine

mammary tumor

multiple drug resistance

Journal Title polish journal of veterinary sciences
Publication Year Start




PMID- 28525328
OWN - NLM
STAT- MEDLINE
DA  - 20170519
DCOM- 20170629
LR  - 20170629
IS  - 1505-1773 (Print)
IS  - 1505-1773 (Linking)
VI  - 20
IP  - 1
DP  - 2017 Mar 28
TI  - Establishment of 5-Fluorouracil-resistant canine mammary tumor cell line.
PG  - 103-110
LID - 10.1515/pjvs-2017-0014 [doi]
LID - /j/pjvs.2017.20.issue-1/pjvs-2017-0014/pjvs-2017-0014.xml [pii]
AB  - Canine mammary tumors are the most common neoplasms in intact female dogs. The
      surgery cannot always solve the problem, chemotherapy are recommend to these
      patients. However, chemotherapy could always fail because of multidrug resistance
      (MDR). Through stepwise increasing 5-Fluorouracil (5-FU) concentration in the
      culture medium, a 5-FU-resistant canine mammary tumor cell line CMT7364/5-FU was 
      established to disclose the molecular mechanism of the drug resistance. Cell
      morphology, cell sensitivity to drugs, growth curves, expression of proteins, and
      chemo-sensitivity in vivo were compared between the parental cell line and
      resistant cell line. As compared it to its parental cell line (CMT7364),
      CMT7364/5-FU showed different morphology, cross-resistant to other chemo-drugs
      and a prolonged population doubling time (PDT). The drug efflux pump proteins
      (ABCB1 and ABCG2) in CMT7364/5-FU were up-regulated. In vivo, the similar result 
      revealed that CMT7364/5-FU cell line was more resistant to 5-FU. In conclusion, a
      5-FU-resistant canine mammary tumor cell line (CMT7364/5-FU) was successfully
      established, it can serve as a good model for researching the mechanism of MDR
      and screening effective agents to reverse drug resistance.
FAU - Zhou, B
AU  - Zhou B
AD  - .
FAU - Zhang, D
AU  - Zhang D
AD  - .
FAU - Pei, S M
AU  - Pei SM
AD  - .
FAU - Zhang, H
AU  - Zhang H
AD  - .
FAU - Du, H C
AU  - Du HC
AD  - .
FAU - Jin, Y P
AU  - Jin YP
AD  - .
FAU - Lin, D G
AU  - Lin DG
AD  - .
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pol J Vet Sci
JT  - Polish journal of veterinary sciences
JID - 101125473
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 0 (Neoplasm Proteins)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Antimetabolites, Antineoplastic/*pharmacology
MH  - Cell Line, Tumor
MH  - Dog Diseases/*pathology
MH  - Dogs
MH  - *Drug Resistance, Neoplasm
MH  - Female
MH  - Fluorouracil/*pharmacology
MH  - Gene Expression Regulation, Neoplastic
MH  - Mammary Neoplasms, Animal/*drug therapy
MH  - Neoplasm Proteins/genetics/metabolism
OTO - NOTNLM
OT  - 5-Fluorouracil
OT  - ABC proteins
OT  - canine
OT  - mammary tumor
OT  - multiple drug resistance
EDAT- 2017/05/20 06:00
MHDA- 2017/07/01 06:00
CRDT- 2017/05/20 06:00
AID - 10.1515/pjvs-2017-0014 [doi]
AID - /j/pjvs.2017.20.issue-1/pjvs-2017-0014/pjvs-2017-0014.xml [pii]
PST - ppublish
SO  - Pol J Vet Sci. 2017 Mar 28;20(1):103-110. doi: 10.1515/pjvs-2017-0014.