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Construction of Lactococcus lactis expressing secreted and anchored Eimeria tenella 3-1E protein and comparison of protective immunity against homologous challenge.

Abstract Two novel plasmids pTX8048-SP-Δ3-1E and pTX8048-SP-NAΔ3-1E-CWA were constructed. The plasmids were respectively electrotransformed into L. lactis NZ9000 to generate strain of L. lactis/pTX8048-SP-Δ3-1E in which 3-1E protein was expressed in secretion, and L. lactis/pTX8048-SP-NAΔ3-1E-CWA on which 3-1E protein was covalently anchored to the surface of bacteria cells. The expression of target proteins were examined by Western blot. The live lactococci expressing secreted 3-1E protein, anchored 3-1E protein, and cytoplasmic 3-1E protein was administered orally to chickens respectively, and the protective immunity and efficacy were compared by animal experiment. The results showed oral immunization to chickens with recombinant lactococci expressing anchored 3-1E protein elicited high 3-1E-specific serum IgG, increased high proportion of CD4(+) and CD8α(+) cells in spleen, alleviated average lesion score in cecum, decreased the oocyst output per chicken compared to lactococci expressing cytoplasmic or secreted 3-1E protein. Taken together, these findings indicated the surface anchored Eimeria protein displayed by L. lacits can induce protective immunity and partial protection against homologous infection.
PMID
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Protective effects of oral immunization with live Lactococcus lactis expressing Eimeria tenella 3-1E protein.

Authors

Mayor MeshTerms
Keywords

Anchored protein

E. tenella

Lactococcus lactis

Protective immunity

Journal Title experimental parasitology
Publication Year Start




PMID- 28526337
OWN - NLM
STAT- MEDLINE
DA  - 20170520
DCOM- 20170622
LR  - 20170622
IS  - 1090-2449 (Electronic)
IS  - 0014-4894 (Linking)
VI  - 178
DP  - 2017 Jul
TI  - Construction of Lactococcus lactis expressing secreted and anchored Eimeria
      tenella 3-1E protein and comparison of protective immunity against homologous
      challenge.
PG  - 14-20
LID - S0014-4894(16)30319-8 [pii]
LID - 10.1016/j.exppara.2017.05.001 [doi]
AB  - Two novel plasmids pTX8048-SP-Delta3-1E and pTX8048-SP-NADelta3-1E-CWA were
      constructed. The plasmids were respectively electrotransformed into L. lactis
      NZ9000 to generate strain of L. lactis/pTX8048-SP-Delta3-1E in which 3-1E protein
      was expressed in secretion, and L. lactis/pTX8048-SP-NADelta3-1E-CWA on which
      3-1E protein was covalently anchored to the surface of bacteria cells. The
      expression of target proteins were examined by Western blot. The live lactococci 
      expressing secreted 3-1E protein, anchored 3-1E protein, and cytoplasmic 3-1E
      protein was administered orally to chickens respectively, and the protective
      immunity and efficacy were compared by animal experiment. The results showed oral
      immunization to chickens with recombinant lactococci expressing anchored 3-1E
      protein elicited high 3-1E-specific serum IgG, increased high proportion of CD4+ 
      and CD8alpha+ cells in spleen, alleviated average lesion score in cecum,
      decreased the oocyst output per chicken compared to lactococci expressing
      cytoplasmic or secreted 3-1E protein. Taken together, these findings indicated
      the surface anchored Eimeria protein displayed by L. lacits can induce protective
      immunity and partial protection against homologous infection.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Ma, Chunli
AU  - Ma C
AD  - College of Food Science, Northeast Agricultural University, NO. 59 Mucai Street, 
      Harbin 150030, China.
FAU - Zhang, Lili
AU  - Zhang L
AD  - College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai
      Street, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals 
      and Comparative Medicine, NO. 59 Mucai Street, Harbin 150030, China.
FAU - Gao, Mingyang
AU  - Gao M
AD  - College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai
      Street, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals 
      and Comparative Medicine, NO. 59 Mucai Street, Harbin 150030, China.
FAU - Ma, Dexing
AU  - Ma D
AD  - College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai
      Street, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals 
      and Comparative Medicine, NO. 59 Mucai Street, Harbin 150030, China. Electronic
      address: [email protected]
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170517
PL  - United States
TA  - Exp Parasitol
JT  - Experimental parasitology
JID - 0370713
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (DNA Primers)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Protozoan Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Blotting, Western
MH  - Chickens
MH  - Coccidiosis/prevention & control/*veterinary
MH  - DNA Primers/chemistry
MH  - Eimeria tenella/chemistry/*immunology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Flow Cytometry
MH  - Immunity, Cellular
MH  - Immunization/methods
MH  - Immunoglobulin G/blood
MH  - Lactococcus lactis/immunology/*metabolism
MH  - Plasmids/genetics/metabolism
MH  - Poultry Diseases/parasitology/*prevention & control
MH  - Protozoan Proteins/immunology/isolation & purification/*metabolism
MH  - Random Allocation
MH  - Specific Pathogen-Free Organisms
MH  - Spleen/cytology/immunology
OTO - NOTNLM
OT  - Anchored protein
OT  - E. tenella
OT  - Lactococcus lactis
OT  - Protective immunity
EDAT- 2017/05/21 06:00
MHDA- 2017/06/24 06:00
CRDT- 2017/05/21 06:00
PHST- 2016/11/16 [received]
PHST- 2017/05/08 [revised]
PHST- 2017/05/16 [accepted]
AID - S0014-4894(16)30319-8 [pii]
AID - 10.1016/j.exppara.2017.05.001 [doi]
PST - ppublish
SO  - Exp Parasitol. 2017 Jul;178:14-20. doi: 10.1016/j.exppara.2017.05.001. Epub 2017 
      May 17.