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Molecular detecting of piroplasms in feeding and questing Ixodes ricinus ticks

Abstract The purpose of this study was to detect piroplasms, which are pathogens of veterinary and zoonotic importance in ticks, that were collected from ponies and field vegetation and to determine the role of Shetland ponies as potential reservoir hosts for piroplasms. A total of 1737 feeding and 371 questing Ixodes ricinus collected from horses or vegetation were tested for the presence of Babesia and Theileria DNA. Piroplasm 18S rRNA gene amplification was conducted, and the obtained amplicons were sequenced. Babesia DNA was detected in only three ticks (one tick collected from a pony and two collected from vegetation), and all of the obtained sequences had 100% similarity to B. divergens. Theileria DNA was not present in the examined ticks. Thus, the above results indicate that ponies are probably not essential hosts for the detected species of piroplasms. Piroplasm species typical for horses (Babesia caballi and Theileria equi) were not detected because I. ricinus is not their vector. The low infection rate of I. ricinus with B. divergens shows that the disease risk for the local horse population and people associated with pony horses is low, but it demonstrates their possible role as a source of human infection in northern Poland.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title annals of parasitology
Publication Year Start




PMID- 28570038
OWN - NLM
STAT- MEDLINE
DA  - 20170601
DCOM- 20170706
LR  - 20170706
IS  - 2299-0631 (Print)
IS  - 2299-0631 (Linking)
VI  - 63
IP  - 1
DP  - 2017
TI  - Molecular detecting of piroplasms in feeding and questing Ixodes ricinus ticks
PG  - 21-26
LID - 10.17420/ap6301.80 [doi]
AB  - The purpose of this study was to detect piroplasms, which are pathogens of
      veterinary and zoonotic importance in ticks, that were collected from ponies and 
      field vegetation and to determine the role of Shetland ponies as potential
      reservoir hosts for piroplasms. A total of 1737 feeding and 371 questing Ixodes
      ricinus collected from horses or vegetation were tested for the presence of
      Babesia and Theileria DNA. Piroplasm 18S rRNA gene amplification was conducted,
      and the obtained amplicons were sequenced. Babesia DNA was detected in only three
      ticks (one tick collected from a pony and two collected from vegetation), and all
      of the obtained sequences had 100% similarity to B. divergens. Theileria DNA was 
      not present in the examined ticks. Thus, the above results indicate that ponies
      are probably not essential hosts for the detected species of piroplasms.
      Piroplasm species typical for horses (Babesia caballi and Theileria equi) were
      not detected because I. ricinus is not their vector. The low infection rate of I.
      ricinus with B. divergens shows that the disease risk for the local horse
      population and people associated with pony horses is low, but it demonstrates
      their possible role as a source of human infection in northern Poland.
FAU - Adamska, Malgorzata
AU  - Adamska M
AD  - Department of Genetics, Faculty of Biology, Szczecin University, ul. Felczaka 3c,
      71-412 Szczecin, Poland
FAU - Skotarczak, Bogumila
AU  - Skotarczak B
AD  - Department of Genetics, Faculty of Biology, Szczecin University, ul. Felczaka 3c,
      71-412 Szczecin, Poland
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Ann Parasitol
JT  - Annals of parasitology
JID - 101593588
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Animals
MH  - Babesia/genetics/*isolation & purification
MH  - DNA, Protozoan/isolation & purification
MH  - Feeding Behavior/*physiology
MH  - Horse Diseases/*parasitology
MH  - Horses
MH  - Ixodes/*parasitology
MH  - Tick Infestations/*veterinary
EDAT- 2017/06/02 06:00
MHDA- 2017/07/07 06:00
CRDT- 2017/06/02 06:00
PST - ppublish
SO  - Ann Parasitol. 2017;63(1):21-26. doi: 10.17420/ap6301.80.