PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 28586923
OWN - NLM
STAT- MEDLINE
DA  - 20170606
DCOM- 20170711
LR  - 20170713
IS  - 1539-3704 (Electronic)
IS  - 0003-4819 (Linking)
VI  - 167
IP  - 1
DP  - 2017 Jul 04
TI  - Presence of Human Hepegivirus-1 in a Cohort of People Who Inject Drugs.
PG  - 1-7
LID - 10.7326/M17-0085 [doi]
AB  - Background: Next-generation metagenomic sequencing (NGMS) has opened new
      frontiers in microbial discovery but has been clinically characterized in only a 
      few settings. Objective: To explore the plasma virome of persons who inject drugs
      and to characterize the sensitivity and accuracy of NGMS compared with
      quantitative clinical standards. Design: Longitudinal and cross-sectional
      studies. Setting: A clinical trial (ClinicalTrials.gov: NCT01285050) and a
      well-characterized cohort study of persons who have injected drugs. Participants:
      Persons co-infected with hepatitis C virus (HCV) and HIV. Measurements: Viral
      nucleic acid in plasma by NGMS and quantitative polymerase chain reaction (PCR). 
      Results: Next-generation metagenomic sequencing generated a total of 600 million 
      reads, which included the expected HIV and HCV RNA sequences. HIV and HCV reads
      were consistently identified only when samples contained more than 10 000
      copies/mL or IU/mL, respectively, as determined by quantitative PCR. A novel RNA 
      virus, human hepegivirus-1 (HHpgV-1), was also detected by NGMS in 4 samples from
      2 persons in the clinical trial. Through use of a quantitative PCR assay for
      HHpgV-1, infection was also detected in 17 (10.9%) of 156 members of a cohort of 
      persons who injected drugs. In these persons, HHpgV-1 viremia persisted for a
      median of at least 4538 days and was associated with detection of other
      bloodborne viruses, such as HCV RNA and SEN virus D. Limitation: The medical
      importance of HHpgV-1 infection is unknown. Conclusion: Although NGMS is
      insensitive for detection of viruses with relatively low plasma nucleic acid
      concentrations, it may have broad potential for discovery of new viral infections
      of possible medical importance, such as HHpgV-1. Primary Funding Source: National
      Institutes of Health.
FAU - Kandathil, Abraham J
AU  - Kandathil AJ
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Breitwieser, Florian P
AU  - Breitwieser FP
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Sachithanandham, Jaiprasath
AU  - Sachithanandham J
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Robinson, Matthew
AU  - Robinson M
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Mehta, Shruti H
AU  - Mehta SH
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Timp, Winston
AU  - Timp W
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Salzberg, Steven L
AU  - Salzberg SL
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Thomas, David L
AU  - Thomas DL
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
FAU - Balagopal, Ashwin
AU  - Balagopal A
AD  - From Johns Hopkins University and Johns Hopkins Bloomberg School of Public
      Health, Baltimore, Maryland.
LA  - eng
PT  - Journal Article
DEP - 20170606
PL  - United States
TA  - Ann Intern Med
JT  - Annals of internal medicine
JID - 0372351
SB  - AIM
SB  - IM
MH  - Coinfection
MH  - Cross-Sectional Studies
MH  - Female
MH  - Genomic Library
MH  - HIV Infections/complications/*virology
MH  - Hepatitis C/complications/*virology
MH  - Hepevirus/genetics/*isolation & purification
MH  - Humans
MH  - Longitudinal Studies
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Analysis, RNA
MH  - Substance Abuse, Intravenous/*virology
MH  - Viremia/*diagnosis
EDAT- 2017/06/07 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/06/07 06:00
AID - 2630764 [pii]
AID - 10.7326/M17-0085 [doi]
PST - ppublish
SO  - Ann Intern Med. 2017 Jul 4;167(1):1-7. doi: 10.7326/M17-0085. Epub 2017 Jun 6.