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Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis.

Abstract Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title revista do instituto de medicina tropical de sao paulo
Publication Year Start




PMID- 28591266
OWN - NLM
STAT- MEDLINE
DA  - 20170607
DCOM- 20170705
LR  - 20170705
IS  - 1678-9946 (Electronic)
IS  - 0036-4665 (Linking)
VI  - 59
DP  - 2017 Jun 01
TI  - Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain 
      Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis.
PG  - e38
LID - S0036-46652017005000220 [pii]
LID - 10.1590/S1678-9946201759038 [doi]
AB  - Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis,
      such as histopathology using biopsy samples, culture techniques, and direct
      search for parasites, have low sensitivity and require invasive collection
      procedures. This study evaluates the efficiency of noninvasive sampling methods
      (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American
      Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had 
      tested positive for leishmaniasis. The outcome of the tests performance on swab
      samples was compatible with PCR results on biopsy samples. The findings have also
      shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests
      (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity 
      of the tests and the fact that the sampling method using swabs affords greater
      patient comfort and safety, it could be said that this method is a promising
      alternative to conventional biopsy-based methods for the molecular diagnosis of
      leishmaniasis.
FAU - Boni, Sara Macente
AU  - Boni SM
AD  - Centro Universitario Cesumar, Departamento de Biomedicina, Maringa, Parana,
      Brazil.
AD  - Universidade de Sao Paulo, Faculdade de Medicina, Departamento de Molestias
      Infecciosas, Sao Paulo, Sao Paulo, Brazil.
FAU - Oyafuso, Luiza Keiko
AU  - Oyafuso LK
AD  - Instituto de Infectologia Emilio Ribas, Sao Paulo, Sao Paulo, Brazil.
FAU - Soler, Rita de Cassia
AU  - Soler RC
AD  - Instituto de Infectologia Emilio Ribas, Sao Paulo, Sao Paulo, Brazil.
FAU - Lindoso, Jose Angelo Lauletta
AU  - Lindoso JAL
AD  - Instituto de Infectologia Emilio Ribas, Sao Paulo, Sao Paulo, Brazil.
AD  - Universidade de Sao Paulo, Instituto de Medicina Tropical de Sao Paulo,
      Laboratorio de Soroepidemiologia e Imunobiologia (LIM 38 HC-FMUSP), Sao Paulo,
      Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170601
PL  - Brazil
TA  - Rev Inst Med Trop Sao Paulo
JT  - Revista do Instituto de Medicina Tropical de Sao Paulo
JID - 7507484
RN  - 0 (DNA, Kinetoplast)
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Biopsy
MH  - DNA, Kinetoplast/*genetics
MH  - DNA, Protozoan/*genetics
MH  - Humans
MH  - Leishmania braziliensis/*genetics
MH  - Leishmaniasis, Cutaneous/*diagnosis
MH  - Polymerase Chain Reaction/methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Skin Tests/methods
MH  - Specimen Handling
PMC - PMC5459545
EDAT- 2017/06/08 06:00
MHDA- 2017/07/06 06:00
CRDT- 2017/06/08 06:00
PHST- 2016/09/23 [received]
PHST- 2017/02/22 [accepted]
AID - S0036-46652017005000220 [pii]
AID - 10.1590/S1678-9946201759038 [doi]
PST - epublish
SO  - Rev Inst Med Trop Sao Paulo. 2017 Jun 1;59:e38. doi: 10.1590/S1678-9946201759038.