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The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease.

Abstract Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitória). One-hundred infected and 57 non-infected animals from Teresina and 100 non-infected animals from Vitória were studied. Animal selection was not dependent on previous serology. The sensitivity (Teresina) and specificity (Teresina and Vitória) were as follows: indirect antibody fluorescence (IFAT) cut-off of 1:40 (IFAT 1:40): 96%, 18%, and 76%; IFAT 1:80: 90%, 33%, and 93%; direct agglutination test (DAT): 96%, 33%, and 98%; fast agglutination screening test (FAST): 93%, 68%, and 100%; immunochromatographic assay with a recombinant rK39 antigen (rK39): 88%, 74%, and 98%; enzyme linked immunosorbent assay (ELISA): 91%, 79%, and 98%; rapid dual-path platform test (TR DPP®): 98%, 60%, and 98%; and blood PCR: 29%, 93%, and 97%, respectively. In the high transmission area, none of the tests adequately discriminated L. infantum-infected from non-infected dogs. However, in the high transmission city, the area under the receiver operating characteristic (ROC) curve of FAST, DAT, ICrK39, ELISA and TR DPP® was high.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title revista do instituto de medicina tropical de sao paulo
Publication Year Start

 



PMID- 28591267
OWN - NLM
STAT- MEDLINE
DA  - 20170607
DCOM- 20170705
LR  - 20170705
IS  - 1678-9946 (Electronic)
IS  - 0036-4665 (Linking)
VI  - 59
DP  - 2017 Jun 01
TI  - The performance of serological tests for Leishmania infantum infection screening 
      in dogs depends on the prevalence of the disease.
PG  - e39
LID - S0036-46652017005000221 [pii]
LID - 10.1590/S1678-9946201759039 [doi]
AB  - Dogs are considered the main reservoir of Leishmania infantum. This protozoan
      causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil.
      Serological tests and polymerase chain reaction (PCR) on peripheral blood were
      performed to identify infected dogs in scenarios of higher and lower prevalence
      of the disease (Teresina and Vitoria). One-hundred infected and 57 non-infected
      animals from Teresina and 100 non-infected animals from Vitoria were studied.
      Animal selection was not dependent on previous serology. The sensitivity
      (Teresina) and specificity (Teresina and Vitoria) were as follows: indirect
      antibody fluorescence (IFAT) cut-off of 1:40 (IFAT 1:40): 96%, 18%, and 76%; IFAT
      1:80: 90%, 33%, and 93%; direct agglutination test (DAT): 96%, 33%, and 98%; fast
      agglutination screening test (FAST): 93%, 68%, and 100%; immunochromatographic
      assay with a recombinant rK39 antigen (rK39): 88%, 74%, and 98%; enzyme linked
      immunosorbent assay (ELISA): 91%, 79%, and 98%; rapid dual-path platform test (TR
      DPP(R)): 98%, 60%, and 98%; and blood PCR: 29%, 93%, and 97%, respectively. In
      the high transmission area, none of the tests adequately discriminated L.
      infantum-infected from non-infected dogs. However, in the high transmission city,
      the area under the receiver operating characteristic (ROC) curve of FAST, DAT,
      ICrK39, ELISA and TR DPP(R) was high.
FAU - Mendonca, Ivete Lopes de
AU  - Mendonca IL
AD  - Universidade Federal do Piaui, Centro de Ciencias Agrarias, Programa de
      Pos-Graduacao em Ciencia Animal, Teresina, Piaui, Brazil.
FAU - Batista, Joilson Ferreira
AU  - Batista JF
AD  - Universidade Federal do Piaui, Centro de Ciencias Agrarias, Programa de
      Pos-Graduacao em Ciencia Animal, Teresina, Piaui, Brazil.
FAU - Schallig, Henk
AU  - Schallig H
AD  - Koninklijk Institut voor de Tropen, Biomedical Research, Amsterdam, Netherlands.
FAU - Cruz, Maria do Socorro Pires E
AU  - Cruz MDSPE
AD  - Universidade Federal do Piaui, Centro de Ciencias Agrarias, Programa de
      Pos-Graduacao em Ciencia Animal, Teresina, Piaui, Brazil.
FAU - Alonso, Diego Peres
AU  - Alonso DP
AD  - Universidade Estadual Paulista, Instituto de Biologia e Biomedicina, Departamento
      de Parasitologia, Botucatu, Sao Paulo, Brazil.
FAU - Ribolla, Paulo Eduardo Martins
AU  - Ribolla PEM
AD  - Universidade Estadual Paulista, Instituto de Biologia e Biomedicina, Departamento
      de Parasitologia, Botucatu, Sao Paulo, Brazil.
FAU - Costa, Dorcas Lamounier
AU  - Costa DL
AD  - Universidade Federal do Piaui, Instituto de Doencas Tropicais Natan Portella,
      Laboratorio de Leishmanioses, Teresina, Piaui, Brazil.
FAU - Costa, Carlos Henrique Nery
AU  - Costa CHN
AD  - Universidade Federal do Piaui, Instituto de Doencas Tropicais Natan Portella,
      Laboratorio de Leishmanioses, Teresina, Piaui, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170601
PL  - Brazil
TA  - Rev Inst Med Trop Sao Paulo
JT  - Revista do Instituto de Medicina Tropical de Sao Paulo
JID - 7507484
RN  - 0 (Antibodies, Protozoan)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/*blood
MH  - Brazil/epidemiology
MH  - Dog Diseases/*diagnosis/epidemiology/parasitology
MH  - Dogs
MH  - Female
MH  - Immunologic Techniques/*methods
MH  - Leishmania infantum/*immunology
MH  - Leishmaniasis, Visceral/diagnosis/epidemiology/*veterinary
MH  - Male
MH  - Mass Screening
MH  - Prevalence
MH  - Sensitivity and Specificity
PMC - PMC5459546
EDAT- 2017/06/08 06:00
MHDA- 2017/07/06 06:00
CRDT- 2017/06/08 06:00
PHST- 2016/09/27 [received]
PHST- 2017/02/22 [accepted]
AID - S0036-46652017005000221 [pii]
AID - 10.1590/S1678-9946201759039 [doi]
PST - epublish
SO  - Rev Inst Med Trop Sao Paulo. 2017 Jun 1;59:e39. doi: 10.1590/S1678-9946201759039.