PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Identification of antigenic Sarcoptes scabiei proteins for use in a diagnostic test and of non-antigenic proteins that may be immunomodulatory.

Abstract Scabies, caused by the mite, Sarcoptes scabiei, infects millions of humans, and many wild and domestic mammals. Scabies mites burrow in the lower stratum corneum of the epidermis of the skin and are the source of substances that are antigenic or modulate aspects of the protective response of the host. Ordinary scabies is a difficult disease to diagnose.
PMID
Related Publications

Identification and heterologous expression of a Sarcoptes scabiei cDNA encoding a structural antigen with immunodiagnostic potential.

The Potential for a Blood Test for Scabies.

Evaluation of an ELISA using recombinant Ssλ20ΔB3 antigen for the serological diagnosis of Sarcoptes scabiei infestation in domestic and wild rabbits.

Skin test and radioallergosorbent test characteristics of scabietic patients.

Serum antibody to Sarcoptes scabiei and house dust mite prior to and during infestation with S. scabiei.

Authors

Mayor MeshTerms

Sarcoptes scabiei

Keywords
Journal Title plos neglected tropical diseases
Publication Year Start




PMID- 28604804
OWN - NLM
STAT- MEDLINE
DA  - 20170612
DCOM- 20170714
LR  - 20170718
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Linking)
VI  - 11
IP  - 6
DP  - 2017 Jun
TI  - Identification of antigenic Sarcoptes scabiei proteins for use in a diagnostic
      test and of non-antigenic proteins that may be immunomodulatory.
PG  - e0005669
LID - 10.1371/journal.pntd.0005669 [doi]
AB  - BACKGROUND: Scabies, caused by the mite, Sarcoptes scabiei, infects millions of
      humans, and many wild and domestic mammals. Scabies mites burrow in the lower
      stratum corneum of the epidermis of the skin and are the source of substances
      that are antigenic or modulate aspects of the protective response of the host.
      Ordinary scabies is a difficult disease to diagnose. OBJECTIVE: The goal of this 
      project was to identify S. scabiei proteins that may be candidate antigens for
      use in a diagnostic test or may be used by the mite to modulate the host's
      protective response. METHODS: An aqueous extract of S. scabiei was separated by
      2-dimensional electrophoresis and proteins were identified by mass spectrometry. 
      A parallel immunoblot was probed with serum from patients with ordinary scabies
      to identify IgM and/or IgG-binding antigens. The genes coding for 23 selected
      proteins were cloned into E. coli and the expressed recombinant proteins were
      screened with serum from patients with confirmed ordinary scabies. RESULTS: We
      identified 50 different proteins produced by S. scabiei, 34 of which were not
      previously identified, and determined that 66% were recognized by patient IgM
      and/or IgG. Fourteen proteins were screened for use in a diagnostic test but none
      possessed enough sensitivity and specificity to be useful. Six of the 9 proteins 
      selected for the possibility that they may be immunomodulatory were not
      recognized by antibodies in patient serum. CONCLUSIONS: Thirty-three proteins
      that bound IgM and/or IgG from the serum of patients with ordinary scabies were
      identified. None of the 14 tested were useful for inclusion in a diagnostic test.
      The identities of 16 proteins that are not recognized as antigens by infected
      patients were also determined. These could be among the molecules that are
      responsible for this mite's ability to modulate its host's innate and adaptive
      immune responses.
FAU - Morgan, Marjorie S
AU  - Morgan MS
AD  - Department of Biological Sciences, Wright State University, Dayton, OH, United
      States of America.
FAU - Rider, S Dean Jr
AU  - Rider SD Jr
AD  - Department of Biological Sciences, Wright State University, Dayton, OH, United
      States of America.
FAU - Arlian, Larry G
AU  - Arlian LG
AD  - Department of Biological Sciences, Wright State University, Dayton, OH, United
      States of America.
LA  - eng
GR  - R01 AI017252/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20170612
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
RN  - 0 (Antigens)
SB  - IM
MH  - Animals
MH  - Antigens/*immunology
MH  - Cloning, Molecular
MH  - Escherichia coli
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunomodulation
MH  - *Sarcoptes scabiei
MH  - Scabies/*diagnosis
PMC - PMC5481144
EDAT- 2017/06/13 06:00
MHDA- 2017/07/15 06:00
CRDT- 2017/06/13 06:00
PHST- 2017/04/28 [received]
PHST- 2017/05/29 [accepted]
PHST- 2017/06/22 [revised]
AID - 10.1371/journal.pntd.0005669 [doi]
AID - PNTD-D-17-00680 [pii]
PST - epublish
SO  - PLoS Negl Trop Dis. 2017 Jun 12;11(6):e0005669. doi:
      10.1371/journal.pntd.0005669. eCollection 2017 Jun.