PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Demethoxycurcumin in combination with ultraviolet radiation B induces apoptosis through the mitochondrial pathway and caspase activation in A431 and HaCaT cells.

Abstract Photodynamic therapy is widely used in the clinical treatment of tumors, especially skin cancers. It has been reported that the photosensitizer curcumin, in combination with ultraviolet radiation B, induces HaCaT cell apoptosis, and this effect may be due to the activation of caspase pathways. In this study, we examined the photodynamic effects of demethoxycurcumin, a more stable analogue of curcumin, to determine whether it could induce apoptosis in skin cancer cells. We investigated the effects of a combination of ultraviolet radiation B and demethoxycurcumin on apoptotic cell death in A431 and HaCaT cells and determined the molecular mechanism of action. Our results showed increased apoptosis with a combination of ultraviolet radiation B with demethoxycurcumin, as compared to ultraviolet radiation B or demethoxycurcumin alone. The combination of ultraviolet radiation B irradiation with demethoxycurcumin synergistically induced apoptotic cell death in A431 and HaCaT cells through activation of p53 and caspase pathways, as well as through upregulation of Bax and p-p65 expression and downregulation of Bcl-2, Mcl-1, and nuclear factor-κB expression. In addition, we found that reactive oxygen species significantly increased with treatment, and mitochondrial membrane potential depolarization was remarkably enhanced. In conclusion, our data indicate that demethoxycurcumin may be a promising photosensitizer for use in photodynamic therapy to induce apoptosis in skin cancer cells.
PMID
Related Publications

Photodynamic therapy-induced apoptosis in epidermoid carcinoma cells. Reactive oxygen species and mitochondrial inner membrane permeabilization.

UVB-induced anti-survival and pro-apoptotic effects on HaCaT human keratinocytes via caspase- and PKC-dependent downregulation of PKB, HIAP-1, Mcl-1, XIAP and ER stress.

The ethyl acetate fraction of Sargassum muticum attenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes.

Combination treatment with photodynamic therapy and curcumin induces mitochondria-dependent apoptosis in AMC-HN3 cells.

Photosensitizer effect of curcumin on UVB-irradiated HaCaT cells through activation of caspase pathways.

Authors

Mayor MeshTerms

Photochemotherapy

Keywords

Demethoxycurcumin

apoptosis

photodynamic therapy

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28618944
OWN - NLM
STAT- MEDLINE
DA  - 20170616
DCOM- 20170622
LR  - 20170622
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Demethoxycurcumin in combination with ultraviolet radiation B induces apoptosis
      through the mitochondrial pathway and caspase activation in A431 and HaCaT cells.
PG  - 1010428317706216
LID - 10.1177/1010428317706216 [doi]
AB  - Photodynamic therapy is widely used in the clinical treatment of tumors,
      especially skin cancers. It has been reported that the photosensitizer curcumin, 
      in combination with ultraviolet radiation B, induces HaCaT cell apoptosis, and
      this effect may be due to the activation of caspase pathways. In this study, we
      examined the photodynamic effects of demethoxycurcumin, a more stable analogue of
      curcumin, to determine whether it could induce apoptosis in skin cancer cells. We
      investigated the effects of a combination of ultraviolet radiation B and
      demethoxycurcumin on apoptotic cell death in A431 and HaCaT cells and determined 
      the molecular mechanism of action. Our results showed increased apoptosis with a 
      combination of ultraviolet radiation B with demethoxycurcumin, as compared to
      ultraviolet radiation B or demethoxycurcumin alone. The combination of
      ultraviolet radiation B irradiation with demethoxycurcumin synergistically
      induced apoptotic cell death in A431 and HaCaT cells through activation of p53
      and caspase pathways, as well as through upregulation of Bax and p-p65 expression
      and downregulation of Bcl-2, Mcl-1, and nuclear factor-kappaB expression. In
      addition, we found that reactive oxygen species significantly increased with
      treatment, and mitochondrial membrane potential depolarization was remarkably
      enhanced. In conclusion, our data indicate that demethoxycurcumin may be a
      promising photosensitizer for use in photodynamic therapy to induce apoptosis in 
      skin cancer cells.
FAU - Xin, Yong
AU  - Xin Y
AD  - 1 Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
FAU - Huang, Qian
AU  - Huang Q
AD  - 1 Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
FAU - Zhang, Pei
AU  - Zhang P
AD  - 1 Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
FAU - Guo, Wen Wen
AU  - Guo WW
AD  - 1 Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
FAU - Zhang, Long Zhen
AU  - Zhang LZ
AD  - 1 Department of Radiotherapy, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
FAU - Jiang, Guan
AU  - Jiang G
AD  - 2 Department of Dermatology, Affiliated Hospital of Xuzhou Medical College,
      Xuzhou, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
RN  - IT942ZTH98 (Curcumin)
RN  - W2F8059T80 (demethoxycurcumin)
SB  - IM
MH  - Caspase 3/biosynthesis
MH  - Caspase 9/biosynthesis
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects/radiation effects
MH  - Curcumin/administration & dosage/*analogs & derivatives
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mitochondria/drug effects/radiation effects
MH  - Neoplasm Proteins/biosynthesis
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/administration & dosage
MH  - Reactive Oxygen Species
MH  - Signal Transduction/drug effects/radiation effects
MH  - Skin Neoplasms/*drug therapy/genetics/pathology/*radiotherapy
MH  - Ultraviolet Rays
OTO - NOTNLM
OT  - Demethoxycurcumin
OT  - apoptosis
OT  - photodynamic therapy
EDAT- 2017/06/18 06:00
MHDA- 2017/06/24 06:00
CRDT- 2017/06/17 06:00
AID - 10.1177/1010428317706216 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317706216. doi: 10.1177/1010428317706216.