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Amelioration of Dalton's lymphoma-induced angiogenesis by melatonin.

Abstract For tumor to grow beyond 1-2 mm(3) size, tumor recruits new blood vessels referred as angiogenesis; therefore, targeting angiogenesis can be a promising strategy to suppress cancer progression. In this study, in order to develop a good angiogenesis model, we investigated effect of Dalton's lymphoma on angiogenesis and further monitored the role of melatonin on regulation of angiogenesis. To evaluate angiogenesis, endothelial cells were isolated from main thoracic aorta and cultured in vitro in the presence or absence of Dalton's lymphoma supplemented with or without melatonin to monitor their role on its proliferation and migration, a hallmark of angiogenesis. Chick chorioallantoic membrane as well as mice mesentery which allows in vivo studies of tumor angiogenesis and testing of anti-angiogenic molecules was used to validate the in vitro analysis. To further extend our understanding about the regulation of the angiogenesis, we evaluated expression of tissue inhibitor of metalloproteinases 3, vascular endothelial growth factor, vascular endothelial growth factor receptor, and fibroblast growth factor in Dalton's lymphoma cells and mesentery by semiquantitative and quantitative reverse transcription polymerase chain reaction analysis. Dalton's lymphoma ascites induced significant increase in endothelial cell proliferation, migration, and sprouting of the tertiary branching in chorioallantoic membrane and mesentery of Dalton's lymphoma-bearing mice, whereas melatonin treatment led to their inhibition in a dose-dependent manner. Semiquantitative and quantitative reverse transcription polymerase chain reaction analysis of melatonin-treated Dalton's lymphoma cells and mesentery tissue clearly demonstrated restoration of angiogenesis-related genes tissue inhibitor of metalloproteinases 3 and reduction of vascular endothelial growth factor, vascular endothelial growth factor receptor, and fibroblast growth factor messenger RNA expression. Taken together, our results strongly demonstrate that Dalton's lymphoma provides pro-angiogenic environment leading to significant increase in angiogenesis, and further melatonin treatment reduced the Dalton's lymphoma ascites-induced angiogenesis implying that Dalton's lymphoma can serve as a very good model to study angiogenesis as well as for screening of drugs that can target angiogenesis.
PMID
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Authors

Mayor MeshTerms
Keywords

Vascular endothelial growth factor

chorioallantoic membrane

endothelial cells

fibroblast growth factor

mice mesentery assay

tissue inhibitor of metalloproteinases 3

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28618962
OWN - NLM
STAT- MEDLINE
DA  - 20170616
DCOM- 20170622
LR  - 20170622
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Amelioration of Dalton's lymphoma-induced angiogenesis by melatonin.
PG  - 1010428317705758
LID - 10.1177/1010428317705758 [doi]
AB  - For tumor to grow beyond 1-2 mm3 size, tumor recruits new blood vessels referred 
      as angiogenesis; therefore, targeting angiogenesis can be a promising strategy to
      suppress cancer progression. In this study, in order to develop a good
      angiogenesis model, we investigated effect of Dalton's lymphoma on angiogenesis
      and further monitored the role of melatonin on regulation of angiogenesis. To
      evaluate angiogenesis, endothelial cells were isolated from main thoracic aorta
      and cultured in vitro in the presence or absence of Dalton's lymphoma
      supplemented with or without melatonin to monitor their role on its proliferation
      and migration, a hallmark of angiogenesis. Chick chorioallantoic membrane as well
      as mice mesentery which allows in vivo studies of tumor angiogenesis and testing 
      of anti-angiogenic molecules was used to validate the in vitro analysis. To
      further extend our understanding about the regulation of the angiogenesis, we
      evaluated expression of tissue inhibitor of metalloproteinases 3, vascular
      endothelial growth factor, vascular endothelial growth factor receptor, and
      fibroblast growth factor in Dalton's lymphoma cells and mesentery by
      semiquantitative and quantitative reverse transcription polymerase chain reaction
      analysis. Dalton's lymphoma ascites induced significant increase in endothelial
      cell proliferation, migration, and sprouting of the tertiary branching in
      chorioallantoic membrane and mesentery of Dalton's lymphoma-bearing mice, whereas
      melatonin treatment led to their inhibition in a dose-dependent manner.
      Semiquantitative and quantitative reverse transcription polymerase chain reaction
      analysis of melatonin-treated Dalton's lymphoma cells and mesentery tissue
      clearly demonstrated restoration of angiogenesis-related genes tissue inhibitor
      of metalloproteinases 3 and reduction of vascular endothelial growth factor,
      vascular endothelial growth factor receptor, and fibroblast growth factor
      messenger RNA expression. Taken together, our results strongly demonstrate that
      Dalton's lymphoma provides pro-angiogenic environment leading to significant
      increase in angiogenesis, and further melatonin treatment reduced the Dalton's
      lymphoma ascites-induced angiogenesis implying that Dalton's lymphoma can serve
      as a very good model to study angiogenesis as well as for screening of drugs that
      can target angiogenesis.
FAU - Kumari, Rani
AU  - Kumari R
AD  - Department of Zoology, University of Delhi, Delhi, India.
FAU - Rawat, Kavita
AU  - Rawat K
AD  - Department of Zoology, University of Delhi, Delhi, India.
FAU - Kumari, Anupma
AU  - Kumari A
AD  - Department of Zoology, University of Delhi, Delhi, India.
FAU - Shrivastava, Anju
AU  - Shrivastava A
AD  - Department of Zoology, University of Delhi, Delhi, India.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Blood Vessels/drug effects/pathology
MH  - Carcinogenesis/drug effects
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Disease Models, Animal
MH  - Endothelial Cells/pathology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Lymphoma/*drug therapy/genetics/pathology
MH  - Melatonin/administration & dosage
MH  - Mice
MH  - Neoplasm Proteins/genetics
MH  - Neovascularization, Pathologic/*drug therapy/genetics/pathology
MH  - Organ Culture Techniques
MH  - RNA, Messenger/genetics
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - Vascular endothelial growth factor
OT  - chorioallantoic membrane
OT  - endothelial cells
OT  - fibroblast growth factor
OT  - mice mesentery assay
OT  - tissue inhibitor of metalloproteinases 3
EDAT- 2017/06/18 06:00
MHDA- 2017/06/24 06:00
CRDT- 2017/06/17 06:00
AID - 10.1177/1010428317705758 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317705758. doi: 10.1177/1010428317705758.