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Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens.

Abstract The fusion (F) protein of Newcastle disease virus (NDV) affects viral infection and pathogenicity through mediating membrane fusion. Previously, we found NDV with increased fusogenic activity in which contained T458D or G459D mutation in the F protein. Here, we investigated the effects of these two mutations on viral infection, fusogenicity and pathogenicity. Syncytium formation assays indicated that T458D or G459D increased the F protein cleavage activity and enhanced cell fusion with or without the presence of HN protein. The T458D- or G459D-mutated NDV resulted in a decrease in virus replication or release from cells. The animal study showed that the pathogenicity of the mutated NDVs was attenuated in chickens. These results indicate that these two single mutations in F altered or diminished the requirement of HN for promoting membrane fusion. The increased fusogenic activity may disrupt the cellular machinery and consequently decrease the virus replication and pathogenicity in chickens.
PMID
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Authors

Mayor MeshTerms

Mutation, Missense

Virus Internalization

Keywords

Fusion protein

Independent fusion

Mutation

Newcastle disease virus

Pathogenicity

Journal Title virology
Publication Year Start




PMID- 28646649
OWN - NLM
STAT- MEDLINE
DA  - 20170624
DCOM- 20170721
LR  - 20170721
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 509
DP  - 2017 Sep
TI  - Two single mutations in the fusion protein of Newcastle disease virus confer
      hemagglutinin-neuraminidase independent fusion promotion and attenuate the
      pathogenicity in chickens.
PG  - 146-151
LID - S0042-6822(17)30197-6 [pii]
LID - 10.1016/j.virol.2017.06.021 [doi]
AB  - The fusion (F) protein of Newcastle disease virus (NDV) affects viral infection
      and pathogenicity through mediating membrane fusion. Previously, we found NDV
      with increased fusogenic activity in which contained T458D or G459D mutation in
      the F protein. Here, we investigated the effects of these two mutations on viral 
      infection, fusogenicity and pathogenicity. Syncytium formation assays indicated
      that T458D or G459D increased the F protein cleavage activity and enhanced cell
      fusion with or without the presence of HN protein. The T458D- or G459D-mutated
      NDV resulted in a decrease in virus replication or release from cells. The animal
      study showed that the pathogenicity of the mutated NDVs was attenuated in
      chickens. These results indicate that these two single mutations in F altered or 
      diminished the requirement of HN for promoting membrane fusion. The increased
      fusogenic activity may disrupt the cellular machinery and consequently decrease
      the virus replication and pathogenicity in chickens.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Ji, Yanhong
AU  - Ji Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Liu, Tao
AU  - Liu T
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Jia, Yane
AU  - Jia Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Liu, Bin
AU  - Liu B
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Yu, Qingzhong
AU  - Yu Q
AD  - United States Department of Agriculture, Agriculture Research Service, US
      National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, 
      GA 30605, USA.
FAU - Cui, Xiaole
AU  - Cui X
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Guo, Fengfeng
AU  - Guo F
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China.
FAU - Chang, Huiyun
AU  - Chang H
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China. Electronic address: [email protected]
FAU - Zhu, Qiyun
AU  - Zhu Q
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR 
      China. Electronic address: [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170621
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Mutant Proteins)
RN  - 0 (Viral Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Cell Fusion
MH  - Chickens
MH  - Disease Models, Animal
MH  - Giant Cells/virology
MH  - Mutant Proteins/genetics/*metabolism
MH  - *Mutation, Missense
MH  - Newcastle Disease/*pathology/virology
MH  - Newcastle disease virus/genetics/pathogenicity/*physiology
MH  - Viral Fusion Proteins/genetics/*metabolism
MH  - Virulence
MH  - *Virus Internalization
MH  - Virus Replication
OTO - NOTNLM
OT  - Fusion protein
OT  - Independent fusion
OT  - Mutation
OT  - Newcastle disease virus
OT  - Pathogenicity
EDAT- 2017/06/25 06:00
MHDA- 2017/07/22 06:00
CRDT- 2017/06/25 06:00
PHST- 2017/02/17 [received]
PHST- 2017/06/14 [revised]
PHST- 2017/06/15 [accepted]
AID - S0042-6822(17)30197-6 [pii]
AID - 10.1016/j.virol.2017.06.021 [doi]
PST - ppublish
SO  - Virology. 2017 Sep;509:146-151. doi: 10.1016/j.virol.2017.06.021. Epub 2017 Jun
      21.