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Inorganic polyphosphate enhances radio-sensitivity in a human non-small cell lung cancer cell line, H1299.

Abstract Inorganic polyphosphate is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. Polyphosphate has been recognized as a potent anti-metastasis reagent. However, the molecular mechanism underlying polyphosphate action on cancer cells is poorly understood. In this study, we investigated the involvement of polyphosphate in radio-sensitivity using a human non-small cell lung cancer cell line, H1299. We found that polyphosphate treatment decreases cellular adenosine triphosphate levels, suggesting a disruption of energy metabolism. We also found that the induction of DNA double-strand breaks was enhanced in polyphosphate-treated cells after X-ray irradiation and colony formation assay revealed that cell survival decreased compared with that of the control groups. These findings suggest that polyphosphate is a promising radio-sensitizer for cancer cells. Therefore, we hypothesized that polyphosphate treatment disrupts adenosine triphosphate-mediated energy transfer for cellular survival and DNA repair, thereby reducing the cellular capability to resist X-ray irradiation.
PMID
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Authors

Mayor MeshTerms
Keywords

Inorganic polyphosphate

adenosine triphosphate

mitochondria

non–small cell lung cancer

radio-sensitivity

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28651489
OWN - NLM
STAT- In-Process
DA  - 20170627
LR  - 20170627
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Inorganic polyphosphate enhances radio-sensitivity in a human non-small cell lung
      cancer cell line, H1299.
PG  - 1010428317705033
LID - 10.1177/1010428317705033 [doi]
AB  - Inorganic polyphosphate is a linear polymer containing tens to hundreds of
      orthophosphate residues linked by high-energy phosphoanhydride bonds.
      Polyphosphate has been recognized as a potent anti-metastasis reagent. However,
      the molecular mechanism underlying polyphosphate action on cancer cells is poorly
      understood. In this study, we investigated the involvement of polyphosphate in
      radio-sensitivity using a human non-small cell lung cancer cell line, H1299. We
      found that polyphosphate treatment decreases cellular adenosine triphosphate
      levels, suggesting a disruption of energy metabolism. We also found that the
      induction of DNA double-strand breaks was enhanced in polyphosphate-treated cells
      after X-ray irradiation and colony formation assay revealed that cell survival
      decreased compared with that of the control groups. These findings suggest that
      polyphosphate is a promising radio-sensitizer for cancer cells. Therefore, we
      hypothesized that polyphosphate treatment disrupts adenosine
      triphosphate-mediated energy transfer for cellular survival and DNA repair,
      thereby reducing the cellular capability to resist X-ray irradiation.
FAU - Tsutsumi, Kaori
AU  - Tsutsumi K
AD  - 1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Matsuya, Yusuke
AU  - Matsuya Y
AD  - 2 Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Sugahara, Tomoki
AU  - Sugahara T
AD  - 3 Sanda City Hospital, Sanda, Japan.
FAU - Tamura, Manami
AU  - Tamura M
AD  - 4 School of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Sawada, Satoshi
AU  - Sawada S
AD  - 4 School of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Fukura, Sagiri
AU  - Fukura S
AD  - 2 Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
FAU - Nakano, Hisashi
AU  - Nakano H
AD  - 5 Hiroshima Heiwa Clinic High-Precision Radiotherapy Center, Hiroshima, Japan.
FAU - Date, Hiroyuki
AU  - Date H
AD  - 1 Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
OTO - NOTNLM
OT  - Inorganic polyphosphate
OT  - adenosine triphosphate
OT  - mitochondria
OT  - non-small cell lung cancer
OT  - radio-sensitivity
EDAT- 2017/06/28 06:00
MHDA- 2017/06/28 06:00
CRDT- 2017/06/28 06:00
AID - 10.1177/1010428317705033 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317705033. doi: 10.1177/1010428317705033.