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Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis.

Abstract MiR-30 family plays an important role in the tumorigenesis of human cancers. The aim of the study is to investigate the role of miR-30d in human colon cancer cell lines and explore the molecular mechanism in the proliferation of colon cancer cells. The expression of miR-30d was determined by real-time polymerase chain reaction assay in colon cancer cell lines (HCT15, HCT116, HT-29, DLD-1, and SW480) and the results demonstrated that miR-30d level was significantly decreased in human colon cancer cell lines, compared with normal colon epithelial cell line. Transfection with miR-30d mimics inhibited cell proliferation, and transfection with miR-30d inhibitors significantly promoted cell viability of colon cancer cells. Furthermore, TargetScan analysis predicted that miR-30d interacted with messenger RNA on its 3' untranslated region of ATG5, phosphoinositide 3-kinase, and Beclin1 to negatively regulate cell autophagy in colon cancer cells. Moreover, transfection with miR-30d induced cell arrest at G2/M phase of HT-29 cells. Overexpression of miR-30d mimics inhibited cell viability probably due to the inhibition of cell autophagy and promotion of cell apoptosis. Thus, MiR-30d inhibited cell autophagy by directly targeting messenger RNA of ATG5, phosphoinositide 3-kinase, and Beclin1 and promoted cell apoptosis of human colon cancer cells. It is helpful to clarify the function of miR-30d in tumorigenesis of human cancers.
PMID
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Authors

Mayor MeshTerms
Keywords

MiR-30d

apoptosis

autophagy

colon cancer

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28651493
OWN - NLM
STAT- MEDLINE
DA  - 20170627
DCOM- 20170710
LR  - 20170713
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell
      autophagy and promoting cell apoptosis.
PG  - 1010428317703984
LID - 10.1177/1010428317703984 [doi]
AB  - MiR-30 family plays an important role in the tumorigenesis of human cancers. The 
      aim of the study is to investigate the role of miR-30d in human colon cancer cell
      lines and explore the molecular mechanism in the proliferation of colon cancer
      cells. The expression of miR-30d was determined by real-time polymerase chain
      reaction assay in colon cancer cell lines (HCT15, HCT116, HT-29, DLD-1, and
      SW480) and the results demonstrated that miR-30d level was significantly
      decreased in human colon cancer cell lines, compared with normal colon epithelial
      cell line. Transfection with miR-30d mimics inhibited cell proliferation, and
      transfection with miR-30d inhibitors significantly promoted cell viability of
      colon cancer cells. Furthermore, TargetScan analysis predicted that miR-30d
      interacted with messenger RNA on its 3' untranslated region of ATG5,
      phosphoinositide 3-kinase, and Beclin1 to negatively regulate cell autophagy in
      colon cancer cells. Moreover, transfection with miR-30d induced cell arrest at
      G2/M phase of HT-29 cells. Overexpression of miR-30d mimics inhibited cell
      viability probably due to the inhibition of cell autophagy and promotion of cell 
      apoptosis. Thus, MiR-30d inhibited cell autophagy by directly targeting messenger
      RNA of ATG5, phosphoinositide 3-kinase, and Beclin1 and promoted cell apoptosis
      of human colon cancer cells. It is helpful to clarify the function of miR-30d in 
      tumorigenesis of human cancers.
FAU - Zhang, Rui
AU  - Zhang R
AD  - 1 Department of Colorectal Surgery, Liaoning Cancer Hospital & Institute, Cancer 
      Hospital of China Medical University, Shenyang, P.R. China.
FAU - Xu, Jian
AU  - Xu J
AD  - 1 Department of Colorectal Surgery, Liaoning Cancer Hospital & Institute, Cancer 
      Hospital of China Medical University, Shenyang, P.R. China.
FAU - Zhao, Jian
AU  - Zhao J
AD  - 1 Department of Colorectal Surgery, Liaoning Cancer Hospital & Institute, Cancer 
      Hospital of China Medical University, Shenyang, P.R. China.
FAU - Bai, Jinghui
AU  - Bai J
AD  - 2 Department of Internal Medicine, Liaoning Cancer Hospital & Institute, Cancer
      Hospital of China Medical University, Shenyang, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (ATG5 protein, human)
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (Beclin-1)
RN  - 0 (MIRN30 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Apoptosis/genetics
MH  - Autophagy/*genetics
MH  - Autophagy-Related Protein 5/biosynthesis/*genetics
MH  - Beclin-1/*genetics
MH  - Carcinogenesis/genetics
MH  - Cell Proliferation/genetics
MH  - Colonic Neoplasms/*genetics/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - HT29 Cells
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Transfection
OTO - NOTNLM
OT  - MiR-30d
OT  - apoptosis
OT  - autophagy
OT  - colon cancer
EDAT- 2017/06/28 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/06/28 06:00
AID - 10.1177/1010428317703984 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317703984. doi: 10.1177/1010428317703984.