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MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling.

Abstract Several studies have revealed that MFG-E8 (milk fat globule-epidermal growth factor 8) is related to tumour development and progression. However, the relationship between MFG-E8 expression and metastasis in colorectal cancer patients and the role of MFG-E8 in colorectal cancer invasion and progression remain unknown. In this study, we performed immunohistochemistry and quantitative real-time polymerase chain reaction to assess MFG-E8 expression in colorectal cancer and adjacent non-cancerous tissues. Colorectal cancer RNAseq data from The Cancer Genome Atlas project were downloaded and MFG-E8 expression was analysed. Gene set enrichment analysis was performed for gene ontology and pathway analysis associated with MFG-E8 expression. For in vitro studies, we used lentivirus-mediated MFG-E8 RNA interference and commercialized recombinant human MFG-E8 to investigate its role in colorectal cancer cell growth, migration and invasion. It seems that MFG-E8 was overexpressed in advanced colorectal cancer tissues compared with early-stage colorectal cancer tissues and adjacent non-cancerous tissues. Correlation analysis revealed that MFG-E8 expression was significantly related to plasma membrane invasion, lymph node metastasis, distant metastasis and tumour-node-metastasis stage. Survival analysis revealed that high MFG-E8 expression predicted a poorer prognosis than low MFG-E8 expression group both in our colorectal cancer cohort and The Cancer Genome Atlas colorectal cancer cohort. In vitro study suggested that MFG-E8 knockdown can suppress the growth of colorectal cancer cells without affecting the expression of the proliferation-related gene Ki67. MFG-E8 knockdown also suppressed colorectal cancer cell migration and invasion, a change accompanied by MMP-2 and MMP-9 downregulation. Moreover, MFG-E8 knockdown induced a shift from mesenchymal makers to epithelial makers, while pretreatment with rhMFG-E8 had the opposite effect. The effect of MFG-E8 on colorectal cancer cell migration, invasion and epithelial-to-mesenchymal was partially dependent on the PI3K/AKT signalling pathway. These findings provide a better understanding of the molecular mechanism underlying colorectal cancer progression and suggest a predictive role for MFG-E8 in colorectal cancer metastasis and prognosis.
PMID
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Authors

Mayor MeshTerms
Keywords

Colorectal cancer

epithelial–mesenchymal transition

invasion

metastasis

milk fat globule–epidermal growth factor 8

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28653875
OWN - NLM
STAT- In-Process
DA  - 20170627
LR  - 20170627
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs
      signalling.
PG  - 1010428317707881
LID - 10.1177/1010428317707881 [doi]
AB  - Several studies have revealed that MFG-E8 (milk fat globule-epidermal growth
      factor 8) is related to tumour development and progression. However, the
      relationship between MFG-E8 expression and metastasis in colorectal cancer
      patients and the role of MFG-E8 in colorectal cancer invasion and progression
      remain unknown. In this study, we performed immunohistochemistry and quantitative
      real-time polymerase chain reaction to assess MFG-E8 expression in colorectal
      cancer and adjacent non-cancerous tissues. Colorectal cancer RNAseq data from The
      Cancer Genome Atlas project were downloaded and MFG-E8 expression was analysed.
      Gene set enrichment analysis was performed for gene ontology and pathway analysis
      associated with MFG-E8 expression. For in vitro studies, we used
      lentivirus-mediated MFG-E8 RNA interference and commercialized recombinant human 
      MFG-E8 to investigate its role in colorectal cancer cell growth, migration and
      invasion. It seems that MFG-E8 was overexpressed in advanced colorectal cancer
      tissues compared with early-stage colorectal cancer tissues and adjacent
      non-cancerous tissues. Correlation analysis revealed that MFG-E8 expression was
      significantly related to plasma membrane invasion, lymph node metastasis, distant
      metastasis and tumour-node-metastasis stage. Survival analysis revealed that high
      MFG-E8 expression predicted a poorer prognosis than low MFG-E8 expression group
      both in our colorectal cancer cohort and The Cancer Genome Atlas colorectal
      cancer cohort. In vitro study suggested that MFG-E8 knockdown can suppress the
      growth of colorectal cancer cells without affecting the expression of the
      proliferation-related gene Ki67. MFG-E8 knockdown also suppressed colorectal
      cancer cell migration and invasion, a change accompanied by MMP-2 and MMP-9
      downregulation. Moreover, MFG-E8 knockdown induced a shift from mesenchymal
      makers to epithelial makers, while pretreatment with rhMFG-E8 had the opposite
      effect. The effect of MFG-E8 on colorectal cancer cell migration, invasion and
      epithelial-to-mesenchymal was partially dependent on the PI3K/AKT signalling
      pathway. These findings provide a better understanding of the molecular mechanism
      underlying colorectal cancer progression and suggest a predictive role for MFG-E8
      in colorectal cancer metastasis and prognosis.
FAU - Zhao, Qiujie
AU  - Zhao Q
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Xu, Lin
AU  - Xu L
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Sun, Xiaoyan
AU  - Sun X
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Zhang, Kai
AU  - Zhang K
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Shen, Huimin
AU  - Shen H
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Tian, Yanan
AU  - Tian Y
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Sun, Fengkai
AU  - Sun F
AD  - 1 Department of Gastroenterology, Shandong Provincial Hospital Affiliated to
      Shandong University, Jinan, China.
FAU - Li, Yanqing
AU  - Li Y
AD  - 2 Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan,
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
OTO - NOTNLM
OT  - Colorectal cancer
OT  - epithelial-mesenchymal transition
OT  - invasion
OT  - metastasis
OT  - milk fat globule-epidermal growth factor 8
EDAT- 2017/06/28 06:00
MHDA- 2017/06/28 06:00
CRDT- 2017/06/28 06:00
AID - 10.1177/1010428317707881 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317707881. doi: 10.1177/1010428317707881.