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MiR-129-5p influences the progression of gastric cancer cells through interacting with SPOCK1.

Abstract The purpose of our study is to clarify the effect of microRNA-129-5p in the progression of human gastric cancer cells by regulating SPOCK1. The expression of microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain reaction in tissues and cell lines. We validated the targeted relationship between microRNA-129-5p and SPOCK1 by dual luciferase reporter gene assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, flow cytometry, transwell, and wound scratch assays were used to analyze the effects of microRNA-129-5p on SGC-7901 cell viability, proliferation, cell cycle and apoptosis, invasiveness, and migration. MicroRNA-129-5p was downregulated while SPOCK1 was upregulated in gastric cancer tissues and cell lines. The result of luciferase reporter gene assay demonstrated that microRNA-129-5p can target SPOCK1 by binding to the 3'untranslated region. The overexpression of microRNA-129-5p or the inhibition of SPOCK1 inhibited SGC-7901 viability, proliferation, migration, and invasion while promoted cell cycle arrest in G0/G1 stage and cell apoptosis. Our results suggested that microRNA-129-5p could directly specifically suppress SPOCK1, which might be one of the potential mechanisms in inhibiting cell processes including viability, proliferation, cell mitosis, migration, and invasiveness of gastric cancer cells.
PMID
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Authors

Mayor MeshTerms
Keywords

Gastric cancer

SPOCK1

microRNA-129-5p

mitosis

viability

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28653880
OWN - NLM
STAT- MEDLINE
DA  - 20170627
DCOM- 20170711
LR  - 20170713
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - MiR-129-5p influences the progression of gastric cancer cells through interacting
      with SPOCK1.
PG  - 1010428317706916
LID - 10.1177/1010428317706916 [doi]
AB  - The purpose of our study is to clarify the effect of microRNA-129-5p in the
      progression of human gastric cancer cells by regulating SPOCK1. The expression of
      microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain 
      reaction in tissues and cell lines. We validated the targeted relationship
      between microRNA-129-5p and SPOCK1 by dual luciferase reporter gene assay.
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation,
      flow cytometry, transwell, and wound scratch assays were used to analyze the
      effects of microRNA-129-5p on SGC-7901 cell viability, proliferation, cell cycle 
      and apoptosis, invasiveness, and migration. MicroRNA-129-5p was downregulated
      while SPOCK1 was upregulated in gastric cancer tissues and cell lines. The result
      of luciferase reporter gene assay demonstrated that microRNA-129-5p can target
      SPOCK1 by binding to the 3'untranslated region. The overexpression of
      microRNA-129-5p or the inhibition of SPOCK1 inhibited SGC-7901 viability,
      proliferation, migration, and invasion while promoted cell cycle arrest in G0/G1 
      stage and cell apoptosis. Our results suggested that microRNA-129-5p could
      directly specifically suppress SPOCK1, which might be one of the potential
      mechanisms in inhibiting cell processes including viability, proliferation, cell 
      mitosis, migration, and invasiveness of gastric cancer cells.
FAU - Yan, Lei
AU  - Yan L
AD  - 1 Department of Histology and Embryology, Mudanjiang Medical University,
      Mudanjiang, People's Republic of China.
FAU - Sun, Kai
AU  - Sun K
AD  - 2 Department of Biology, Mudanjiang Medical University, Mudanjiang, People's
      Republic of China.
FAU - Liu, Yang
AU  - Liu Y
AD  - 3 Department of Pathogenic Microbiology and Immunology, Mudanjiang Medical
      University, Mudanjiang, People's Republic of China.
FAU - Liang, Jun
AU  - Liang J
AD  - 1 Department of Histology and Embryology, Mudanjiang Medical University,
      Mudanjiang, People's Republic of China.
FAU - Cai, Kerui
AU  - Cai K
AD  - 1 Department of Histology and Embryology, Mudanjiang Medical University,
      Mudanjiang, People's Republic of China.
FAU - Gui, Jinqiu
AU  - Gui J
AD  - 3 Department of Pathogenic Microbiology and Immunology, Mudanjiang Medical
      University, Mudanjiang, People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (Proteoglycans)
RN  - 0 (SPOCK1 protein, human)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Cycle Checkpoints/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/*genetics
MH  - Cell Survival/genetics
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Proteoglycans/*biosynthesis/genetics
MH  - Stomach Neoplasms/*genetics/pathology
OTO - NOTNLM
OT  - Gastric cancer
OT  - SPOCK1
OT  - microRNA-129-5p
OT  - mitosis
OT  - viability
EDAT- 2017/06/28 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/06/28 06:00
AID - 10.1177/1010428317706916 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317706916. doi: 10.1177/1010428317706916.