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siRNA-mediated silencing of bFGF gene inhibits the proliferation, migration, and invasion of human pituitary adenoma cells.

Abstract Human pituitary adenoma is one of the most common intracranial tumors with an incidence as high as 16.7%. Recent evidence has hinted a relationship between growth factors of pituitary or hypothalamic origin and proliferation of human pituitary adenoma cells. This study explores the effects of small interfering RNA-mediated silencing of basic fibroblast growth factor gene on the proliferation, migration, and invasion of human pituitary adenoma cells. Human pituitary adenoma tissues were collected to obtain human pituitary adenoma cells. The basic fibroblast growth factor silencing interference plasmid was constructed, and the human pituitary adenoma cells were transfected and assigned as basic fibroblast growth factor-small interfering RNA, negative control-small interfering RNA, and blank groups. The quantitative real-time polymerase chain reaction and Western blotting were carried out to detect the expression of basic fibroblast growth factor, pituitary tumor transforming gene, vascular endothelial growth factor, cluster of differentiation 147, and matrix metalloproteinase 9. Cell Counting Kit-8 assay was conducted to observe cell proliferation at 0, 24, 48, and 72 h. Flow cytometry was used to determine cell cycle. Transwell and scratch test were applied to detect the invasion and migration of pituitary adenoma cells. Protein kinase C activity was detected. In comparison with the blank group, the basic fibroblast growth factor-small interfering RNA group showed reduced messenger RNA and protein expression of basic fibroblast growth factor, reduced cell viability at 24, 48, and 72 h, increased cells in G0/G1 stage, declined cells in S and G2/M stages, decreased number of cell migration, shortened migrating distance, reduced protein kinase C activity, and decreased expression of pituitary tumor transforming gene, vascular endothelial growth factor, cluster of differentiation 147, and matrix metalloproteinase 9. However, the negative control-small interfering RNA group had no evident differences in basic fibroblast growth factor expression, cell viability, cell cycle, number of cell migration, migrating distance, protein kinase C activity, and expression of pituitary tumor transforming gene, vascular endothelial growth factor, cluster of differentiation 147, and matrix metalloproteinase 9 compared with the blank group. The study provides evidence that small interfering RNA-mediated silencing of basic fibroblast growth factor gene inhibits the proliferation, migration, and invasion of human pituitary adenoma cells.
PMID
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Authors

Mayor MeshTerms
Keywords

Small interfering RNA silencing

basic fibroblast growth factor

cell invasiveness

cell migration

cell proliferation

pituitary adenoma cells

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28656882
OWN - NLM
STAT- MEDLINE
DA  - 20170628
DCOM- 20170711
LR  - 20170713
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 6
DP  - 2017 Jun
TI  - siRNA-mediated silencing of bFGF gene inhibits the proliferation, migration, and 
      invasion of human pituitary adenoma cells.
PG  - 1010428317704805
LID - 10.1177/1010428317704805 [doi]
AB  - Human pituitary adenoma is one of the most common intracranial tumors with an
      incidence as high as 16.7%. Recent evidence has hinted a relationship between
      growth factors of pituitary or hypothalamic origin and proliferation of human
      pituitary adenoma cells. This study explores the effects of small interfering
      RNA-mediated silencing of basic fibroblast growth factor gene on the
      proliferation, migration, and invasion of human pituitary adenoma cells. Human
      pituitary adenoma tissues were collected to obtain human pituitary adenoma cells.
      The basic fibroblast growth factor silencing interference plasmid was
      constructed, and the human pituitary adenoma cells were transfected and assigned 
      as basic fibroblast growth factor-small interfering RNA, negative control-small
      interfering RNA, and blank groups. The quantitative real-time polymerase chain
      reaction and Western blotting were carried out to detect the expression of basic 
      fibroblast growth factor, pituitary tumor transforming gene, vascular endothelial
      growth factor, cluster of differentiation 147, and matrix metalloproteinase 9.
      Cell Counting Kit-8 assay was conducted to observe cell proliferation at 0, 24,
      48, and 72 h. Flow cytometry was used to determine cell cycle. Transwell and
      scratch test were applied to detect the invasion and migration of pituitary
      adenoma cells. Protein kinase C activity was detected. In comparison with the
      blank group, the basic fibroblast growth factor-small interfering RNA group
      showed reduced messenger RNA and protein expression of basic fibroblast growth
      factor, reduced cell viability at 24, 48, and 72 h, increased cells in G0/G1
      stage, declined cells in S and G2/M stages, decreased number of cell migration,
      shortened migrating distance, reduced protein kinase C activity, and decreased
      expression of pituitary tumor transforming gene, vascular endothelial growth
      factor, cluster of differentiation 147, and matrix metalloproteinase 9. However, 
      the negative control-small interfering RNA group had no evident differences in
      basic fibroblast growth factor expression, cell viability, cell cycle, number of 
      cell migration, migrating distance, protein kinase C activity, and expression of 
      pituitary tumor transforming gene, vascular endothelial growth factor, cluster of
      differentiation 147, and matrix metalloproteinase 9 compared with the blank
      group. The study provides evidence that small interfering RNA-mediated silencing 
      of basic fibroblast growth factor gene inhibits the proliferation, migration, and
      invasion of human pituitary adenoma cells.
FAU - Zhou, Kai
AU  - Zhou K
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Fan, Yan-Dong
AU  - Fan YD
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Duysenbi, Serick
AU  - Duysenbi S
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Wu, Peng-Fei
AU  - Wu PF
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Feng, Zhao-Hai
AU  - Feng ZH
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Qian, Zheng
AU  - Qian Z
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
FAU - Zhang, Ting-Rong
AU  - Zhang TR
AD  - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical
      University, Urumqi, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (RNA, Small Interfering)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Movement
MH  - Cell Proliferation/*genetics
MH  - Fibroblast Growth Factor 2/antagonists & inhibitors/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Silencing
MH  - Humans
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Pituitary Neoplasms/*genetics/pathology
MH  - RNA, Small Interfering/genetics
OTO - NOTNLM
OT  - Small interfering RNA silencing
OT  - basic fibroblast growth factor
OT  - cell invasiveness
OT  - cell migration
OT  - cell proliferation
OT  - pituitary adenoma cells
EDAT- 2017/06/29 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/06/29 06:00
AID - 10.1177/1010428317704805 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jun;39(6):1010428317704805. doi: 10.1177/1010428317704805.