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PMID- 28675006
OWN - NLM
STAT- MEDLINE
DA  - 20170704
DCOM- 20170807
LR  - 20170807
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 35
IP  - 3
DP  - 2017 Jun 01
TI  - [Construction of a low-pH-sensing system in Streptococcus mutans].
PG  - 239-244
LID - 10.7518/hxkq.2017.03.003 [doi]
AB  - OBJECTIVE: To construct a low-pH-sensing system in Streptococcus mutans (S.
      mutans) and to visually detect the pH in situ. METHODS: Promoter of urease(Pure) 
      and green fluorescence protein (gfp) DNA fragments were amplified by polymerase
      chain reaction (PCR) from the genome of Streptococcus salivarius 57.I and S.
      mutans containing the gfp fragment. The two amplified DNA fragments were ligated 
      together and further integrated into pDL278 to construct the recombinant plasmid 
      pDL278-pure-gfp. This recombinant plasmid was then transformed into S. mutans
      UA159 cells. Subsequently, the intensity of the optical density per unit area of 
      the low-pH-sensing system was measured and compared under different pH conditions
      and different processing times. RESULTS: Pure and gfp DNA fragments were
      amplified successfully with the correct molecule sizes (450 and 717 bp,
      respectively). The recombinant plasmid pDL278-pure-gfp was constructed and
      further verified by PCR and sequencing. The intensity of the optical density per 
      unit area of the low-pH-sensing system increased with decreasing pH and
      increasing processing time. CONCLUSIONS: A low-pH-sensing system was constructed 
      successfully in S. mutans. Our research verified that pure of Streptococcus
      salivarius can function well in S. mutans as an acid induced promoter, and
      provided a new method of detecting the pH of plaque biofilms in situ.
FAU - Di, Kang
AU  - Di K
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041,
      China.
FAU - Yuqing, Li
AU  - Yuqing L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041,
      China.
FAU - Xuedong, Zhou
AU  - Xuedong Z
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041,
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - D
SB  - IM
MH  - *Biofilms
MH  - *Dental Plaque
MH  - Green Fluorescent Proteins
MH  - Hydrogen-Ion Concentration
MH  - *Plasmids
MH  - Promoter Regions, Genetic
MH  - *Streptococcus mutans
MH  - Streptococcus salivarius
OTO - NOTNLM
OT  - Streptococcus mutans
OT  - Streptococcus salivarius
OT  - green fluorescence protein
OT  - promoter
OT  - urease
EDAT- 2017/07/05 06:00
MHDA- 2017/08/08 06:00
CRDT- 2017/07/05 06:00
AID - 10.7518/hxkq.2017.03.003 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Jun 1;35(3):239-244. doi:
      10.7518/hxkq.2017.03.003.