PubTransformer

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PMID- 28682553
OWN - NLM
STAT- MEDLINE
DA  - 20170706
DCOM- 20170807
LR  - 20170807
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 35
IP  - 2
DP  - 2017 Apr 01
TI  - [A preliminarystudyon the autophagy level of human periodontal ligament cells
      regulated by nicotine].
PG  - 198-202
LID - 10.7518/hxkq.2017.02.017 [doi]
AB  - OBJECTIVE: To explore the effect of nicotine on the autophagy level of human
      periodontal ligament cells (hPDLCs). METHODS: Periodontal tissues collected from 
      premolars for orthodontic treatment reasons were used to culture hPDLCs. Western 
      blot analysis was performed to test the most optimal time and concentration of
      nicotine on the autophagy level of the hPDLCs. Transmission electron microscope
      and immunofluorescence observation were carried out to detect the form of
      autophagosomes and expression of autophagy related protein LC3 in hPDLCs under
      this optimal condition. RESULTS: Protein expression of LC3 was up regulated with 
      the 12 h nicotine stimulating. Besides that, the up regulation of the protein
      expression of LC3 was concentration dependent and nicotine with a concentration
      of 1x10(-)(5) mol.L(-)(1) was the most optimal condition. Transmission electron
      microscope and immunofluorescence observations indicated that nicotine would
      activate the autophagy level of hPDLCs by increasing the number of autophagosomes
      and up regulating the expression of autophagy related protein LC3. CONCLUSIONS:
      Nicotine could increase autophagy level of hPDLCs, thus affecting the occurrence 
      and development of smoking related periodontitis.
FAU - Yang, Du
AU  - Yang D
AD  - State Key Laboratory of Military Stomatology, National Clinical Research Center
      for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of
      Pediatric Dentistry, School of Stomatology, The Fourth Military Medical
      University, Xi'an 710032, China.
FAU - Shuai, Yuan
AU  - Shuai Y
AD  - State Key Laboratory of Military Stomatology, National Clinical Research Center
      for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of
      Pediatric Dentistry, School of Stomatology, The Fourth Military Medical
      University, Xi'an 710032, China.
FAU - Zhifei, Zhou
AU  - Zhifei Z
AD  - State Key Laboratory of Military Stomatology, National Clinical Research Center
      for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of
      Pediatric Dentistry, School of Stomatology, The Fourth Military Medical
      University, Xi'an 710032, China.
FAU - Lizheng, Wu
AU  - Lizheng W
AD  - Dept. of Stomatology, Affiliated Hospital of Logistics University of People's
      Armed Police Force, Tianjin 300300, China.
FAU - Lulu, Wang
AU  - Lulu W
AD  - State Key Laboratory of Military Stomatology, National Clinical Research Center
      for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of
      Pediatric Dentistry, School of Stomatology, The Fourth Military Medical
      University, Xi'an 710032, China.
FAU - Xing'an, Wu
AU  - Xing'an W
AD  - Dept. of Microbiology, The Fourth Military Medical University, Xi'an 710032,
      China.
FAU - Xiaojing, Wang
AU  - Xiaojing W
AD  - State Key Laboratory of Military Stomatology, National Clinical Research Center
      for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of
      Pediatric Dentistry, School of Stomatology, The Fourth Military Medical
      University, Xi'an 710032, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (light chain 3, human)
RN  - 6M3C89ZY6R (Nicotine)
SB  - D
SB  - IM
MH  - *Autophagy
MH  - Blotting, Western
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Humans
MH  - Microtubule-Associated Proteins
MH  - Nicotine/*pharmacology
MH  - Nicotinic Agonists/*pharmacology
MH  - *Periodontal Ligament
MH  - *Periodontitis
MH  - Up-Regulation
OTO - NOTNLM
OT  - autophagy
OT  - human periodontal ligament cells
OT  - nicotine
EDAT- 2017/07/07 06:00
MHDA- 2017/08/08 06:00
CRDT- 2017/07/07 06:00
AID - 10.7518/hxkq.2017.02.017 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Apr 1;35(2):198-202. doi:
      10.7518/hxkq.2017.02.017.