PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 28687847
OWN - NLM
STAT- MEDLINE
DA  - 20170708
DCOM- 20170714
LR  - 20170714
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 9
DP  - 2017 Jul 01
TI  - Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit
      Descemet Membrane Endothelial Keratoplasty.
PG  - 3357-3365
LID - 10.1167/iovs.17-21797 [doi]
AB  - Purpose: Develop a silk fibroin (SF)-based artificial endothelial graft for its
      use in a rabbit Descemet membrane endothelial keratoplasty (DMEK). Methods: Human
      and rabbit artificial corneal endothelial grafts were developed through the
      culture of human and rabbit corneal endothelial cells (CECs) on SF films. Rabbit 
      artificial SF endothelial grafts were transplanted in a DMEK surgery into a
      rabbit in vivo model. Results: SF artificial endothelial grafts showed the
      characteristic endothelial markers: zonula occludens (ZO-1) and Na+/K+ ATPase. In
      a rabbit model of DMEK surgery, SF artificial endothelial graft restored the
      corneal transparency and thickness at 6 week of follow-up. Anterior segment
      optical coherence tomography revealed the SF graft as a fully integrated
      component in the corneal tissue, displaying a similar corneal thickness and
      endothelial cell count when compared with its healthy contralateral cornea.
      Histologic analysis showed that the SF artificial endothelial graft was attached 
      and integrated on the surface of the corneal stroma without a significant
      inflammatory reaction, and rabbit CECs consisted in a monolayer that showed their
      characteristic markers ZO-1 and Na+/K+ ATPase, suggesting proper intercellular
      junctions and cellular pump function. Conclusions: We have developed SF films
      with biological properties that supported the growth of rabbit and human CECs,
      which showed normal morphology and characteristic markers; and with mechanical
      properties that allowed its use in a DMEK surgery, proving its in vivo
      functionality in a rabbit model of endothelial dysfunction.
FAU - Vazquez, Natalia
AU  - Vazquez N
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Rodriguez-Barrientos, Carlos A
AU  - Rodriguez-Barrientos CA
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Aznar-Cervantes, Salvador D
AU  - Aznar-Cervantes SD
AD  - Instituto Murciano de Investigacion y Desarrollo Agrario y Alimentario,
      Departamento de Biotecnologia de La Alberca, Murcia, Spain.
FAU - Chacon, Manuel
AU  - Chacon M
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Cenis, Jose L
AU  - Cenis JL
AD  - Instituto Murciano de Investigacion y Desarrollo Agrario y Alimentario,
      Departamento de Biotecnologia de La Alberca, Murcia, Spain.
FAU - Riestra, Ana C
AU  - Riestra AC
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Sanchez-Avila, Ronald M
AU  - Sanchez-Avila RM
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Persinal, Mairobi
AU  - Persinal M
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Brea-Pastor, Agustin
AU  - Brea-Pastor A
AD  - Unidad de Bioterio e Imagen Preclinica, Universidad de Oviedo, Asturias, Spain.
FAU - Fernandez-Vega Cueto, Luis
AU  - Fernandez-Vega Cueto L
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Meana, Alvaro
AU  - Meana A
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
FAU - Merayo-Lloves, Jesus
AU  - Merayo-Lloves J
AD  - Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica,
      Universidad de Oviedo, Asturias, Spain.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Membranes, Artificial)
RN  - 0 (Silk)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Corneal Edema/pathology/*surgery
MH  - Descemet Stripping Endothelial Keratoplasty/*methods
MH  - Disease Models, Animal
MH  - Endothelial Cells/cytology
MH  - Endothelium, Corneal/*transplantation
MH  - *Fibroins
MH  - Humans
MH  - *Membranes, Artificial
MH  - Rabbits
MH  - *Silk
MH  - *Tissue Engineering/methods
EDAT- 2017/07/09 06:00
MHDA- 2017/07/15 06:00
CRDT- 2017/07/09 06:00
AID - 2643336 [pii]
AID - 10.1167/iovs.17-21797 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Jul 1;58(9):3357-3365. doi:
      10.1167/iovs.17-21797.