PubTransformer

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PMID- 28700779
OWN - NLM
STAT- MEDLINE
DA  - 20170712
DCOM- 20170809
LR  - 20170809
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 9
DP  - 2017 Jul 01
TI  - Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and
      Histologic Study.
PG  - 3471-3480
LID - 10.1167/iovs.16-21365 [doi]
AB  - Purpose: To evaluate corneal wound healing after treatment with a new collagen
      crosslinking protocol using rose bengal dye and green light (RGX). Methods: One
      cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter
      circle and, in another group (n = 25), the DE corneas were then stained with 0.1%
      rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes
      (RGX). The contralateral eyes without treatment acted as controls. The animals
      were clinically followed including fluorescein staining and pachymetry. Healing
      events were analyzed after euthanasia at 2, 30, and 60 days. Cell death (TUNEL
      assay), cell proliferation (5-bromo-2'-deoxyuridine incorporation), and cell
      differentiation to myofibroblasts (alpha-SMA labeling) were carried out. In
      addition, loss of keratocytes and subsequent repopulation of the corneal stroma
      were quantified on hematoxylin-eosin-stained sections. Results: Wound closure was
      slower after RGX (4.4 days) then after DE (3.3 days). Cell death was restricted
      to the anterior central stroma, and the cellular decrease did not differ
      significantly between RGX and DE corneas. Cell proliferation in the epithelium
      and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the
      epithelium was complete at day 30, although cell repopulation of the stroma was
      not complete at 60 days. Conclusions: The healing response in corneas after RGX
      is very similar to that observed after DE alone, suggesting that, along with its 
      short treatment time and limited effect on keratocytes, RGX displays good
      potential for clinical cornea stiffening.
FAU - Gallego-Munoz, Patricia
AU  - Gallego-Munoz P
AD  - Departamento de Biologia Celular, Histologia y Farmacologia, GIR de Tecnicas
      Opticas para el Diagnostico, Universidad de Valladolid, Valladolid, Spain.
FAU - Ibares-Frias, Lucia
AU  - Ibares-Frias L
AD  - Departamento de Biologia Celular, Histologia y Farmacologia, GIR de Tecnicas
      Opticas para el Diagnostico, Universidad de Valladolid, Valladolid, Spain.
FAU - Lorenzo, Elvira
AU  - Lorenzo E
AD  - Departamento de Biologia Celular, Histologia y Farmacologia, GIR de Tecnicas
      Opticas para el Diagnostico, Universidad de Valladolid, Valladolid, Spain.
FAU - Marcos, Susana
AU  - Marcos S
AD  - Instituto de Optica, Consejo Superior de Investigaciones Cientificas, Madrid,
      Spain.
FAU - Perez-Merino, Pablo
AU  - Perez-Merino P
AD  - Instituto de Optica, Consejo Superior de Investigaciones Cientificas, Madrid,
      Spain.
FAU - Bekesi, Nandor
AU  - Bekesi N
AD  - Instituto de Optica, Consejo Superior de Investigaciones Cientificas, Madrid,
      Spain.
FAU - Kochevar, Irene E
AU  - Kochevar IE
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical
      School, Boston, Massachusetts, United States.
FAU - Martinez-Garcia, M Carmen
AU  - Martinez-Garcia MC
AD  - Departamento de Biologia Celular, Histologia y Farmacologia, GIR de Tecnicas
      Opticas para el Diagnostico, Universidad de Valladolid, Valladolid, Spain.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Fluorescent Dyes)
RN  - 1ZPG1ELY14 (Rose Bengal)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Collagen/*pharmacology
MH  - Cornea/drug effects/*pathology
MH  - Corneal Injuries/*drug therapy
MH  - Cross-Linking Reagents/*pharmacology
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Dyes/pharmacology
MH  - *Light
MH  - Rabbits
MH  - Rose Bengal/*pharmacology
MH  - Wound Healing/*drug effects
EDAT- 2017/07/13 06:00
MHDA- 2017/08/10 06:00
CRDT- 2017/07/13 06:00
AID - 2644165 [pii]
AID - 10.1167/iovs.16-21365 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Jul 1;58(9):3471-3480. doi:
      10.1167/iovs.16-21365.