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Detection of chronic wasting disease prion seeding activity in deer and elk feces by real-time quaking-induced conversion.

Abstract Chronic wasting disease (CWD) is an emergent prion disease affecting cervid species in North America, Canada, South Korea, and recently, Norway. Detection of CWD has been advanced by techniques that rely on amplification of low levels of prion amyloid to a detectable level. However, the increased sensitivity of amplification assays is often compromised by inhibitors and/or activators in complex biologic samples including body fluids, excreta, or the environment. Here, we adapt real-time quaking-induced conversion conditions to specifically detect CWD prions in fecal samples from both experimentally infected deer and naturally infected elk and estimate environmental contamination. The results have application to detection, surveillance and management of CWD, and potentially to other protein-misfolding diseases.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title the journal of general virology
Publication Year Start




PMID- 28703697
OWN - NLM
STAT- MEDLINE
DA  - 20170713
DCOM- 20170804
LR  - 20170804
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 98
IP  - 7
DP  - 2017 Jul
TI  - Detection of chronic wasting disease prion seeding activity in deer and elk feces
      by real-time quaking-induced conversion.
PG  - 1953-1962
LID - 10.1099/jgv.0.000844 [doi]
AB  - Chronic wasting disease (CWD) is an emergent prion disease affecting cervid
      species in North America, Canada, South Korea, and recently, Norway. Detection of
      CWD has been advanced by techniques that rely on amplification of low levels of
      prion amyloid to a detectable level. However, the increased sensitivity of
      amplification assays is often compromised by inhibitors and/or activators in
      complex biologic samples including body fluids, excreta, or the environment.
      Here, we adapt real-time quaking-induced conversion conditions to specifically
      detect CWD prions in fecal samples from both experimentally infected deer and
      naturally infected elk and estimate environmental contamination. The results have
      application to detection, surveillance and management of CWD, and potentially to 
      other protein-misfolding diseases.
FAU - Henderson, Davin M
AU  - Henderson DM
AD  - 1Prion Research Center, College of Veterinary Medicine and Biomedical Sciences,
      Colorado State University, Fort Collins, CO, USA.
FAU - Tennant, Joanne M
AU  - Tennant JM
AD  - 1Prion Research Center, College of Veterinary Medicine and Biomedical Sciences,
      Colorado State University, Fort Collins, CO, USA.
FAU - Haley, Nicholas J
AU  - Haley NJ
AD  - 2Department of Microbiology and Pathology, Midwestern State University, Glendale,
      AZ, USA.
FAU - Denkers, Nathaniel D
AU  - Denkers ND
AD  - 1Prion Research Center, College of Veterinary Medicine and Biomedical Sciences,
      Colorado State University, Fort Collins, CO, USA.
FAU - Mathiason, Candace K
AU  - Mathiason CK
AD  - 1Prion Research Center, College of Veterinary Medicine and Biomedical Sciences,
      Colorado State University, Fort Collins, CO, USA.
FAU - Hoover, Edward A
AU  - Hoover EA
AD  - 1Prion Research Center, College of Veterinary Medicine and Biomedical Sciences,
      Colorado State University, Fort Collins, CO, USA.
LA  - eng
PT  - Journal Article
DEP - 20170713
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Amyloid)
RN  - 0 (Prion Proteins)
RN  - 0 (Prions)
SB  - IM
MH  - Amyloid/*analysis
MH  - Animals
MH  - Biological Assay/*methods
MH  - Deer
MH  - Feces/*chemistry
MH  - Prion Proteins/*isolation & purification
MH  - Prions/*analysis
MH  - Wasting Disease, Chronic/*diagnosis/pathology
EDAT- 2017/07/14 06:00
MHDA- 2017/08/05 06:00
CRDT- 2017/07/14 06:00
AID - 10.1099/jgv.0.000844 [doi]
PST - ppublish
SO  - J Gen Virol. 2017 Jul;98(7):1953-1962. doi: 10.1099/jgv.0.000844. Epub 2017 Jul
      13.