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A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis.

Abstract Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infected tissues. Although these techniques are highly specific, they require highly skilled health workers and have the inherent risks of all invasive procedures, such as pain and risk of bacterial and fungal super-infection. Therefore, it is essential to reduce discomfort, potential infection and scarring caused by invasive diagnostic approaches especially for children. In this report, we present a novel non-invasive method, that is painless, rapid and user-friendly, using sequential tape strips for sampling and isolation of DNA from the surface of active and healed skin lesions of CL patients. A total of 119 patients suspected of suffering from cutaneous leishmaniasis with different clinical manifestations were recruited and samples were collected both from their lesions and from uninfected areas. In addition, 15 fungal-infected lesions and 54 areas of healthy skin were examined. The duration of sampling is short (less than one minute) and species identification by PCR is highly specific and sensitive. The sequential tape stripping sampling method is a sensitive, non-invasive and cost-effective alternative to traditional diagnostic assays and it is suitable for field studies as well as for use in health care centers.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos neglected tropical diseases
Publication Year Start




PMID- 28704463
OWN - NLM
STAT- MEDLINE
DA  - 20170713
DCOM- 20170731
LR  - 20170731
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Linking)
VI  - 11
IP  - 7
DP  - 2017 Jul
TI  - A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis.
PG  - e0005750
LID - 10.1371/journal.pntd.0005750 [doi]
AB  - Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy 
      and epidemiological studies. Common approaches for Leishmania detection involve
      the invasive collection of specimens for direct identification of amastigotes by 
      microscopy and the culturing of promastigotes from infected tissues. Although
      these techniques are highly specific, they require highly skilled health workers 
      and have the inherent risks of all invasive procedures, such as pain and risk of 
      bacterial and fungal super-infection. Therefore, it is essential to reduce
      discomfort, potential infection and scarring caused by invasive diagnostic
      approaches especially for children. In this report, we present a novel
      non-invasive method, that is painless, rapid and user-friendly, using sequential 
      tape strips for sampling and isolation of DNA from the surface of active and
      healed skin lesions of CL patients. A total of 119 patients suspected of
      suffering from cutaneous leishmaniasis with different clinical manifestations
      were recruited and samples were collected both from their lesions and from
      uninfected areas. In addition, 15 fungal-infected lesions and 54 areas of healthy
      skin were examined. The duration of sampling is short (less than one minute) and 
      species identification by PCR is highly specific and sensitive. The sequential
      tape stripping sampling method is a sensitive, non-invasive and cost-effective
      alternative to traditional diagnostic assays and it is suitable for field studies
      as well as for use in health care centers.
FAU - Taslimi, Yasaman
AU  - Taslimi Y
AD  - Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of
      Iran, Tehran, Iran.
FAU - Sadeghipour, Pardis
AU  - Sadeghipour P
AD  - Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of
      Iran, Tehran, Iran.
FAU - Habibzadeh, Sima
AU  - Habibzadeh S
AD  - Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of
      Iran, Tehran, Iran.
FAU - Mashayekhi, Vahid
AU  - Mashayekhi V
AD  - Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
FAU - Mortazavi, Hossien
AU  - Mortazavi H
AD  - Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Muller, Ingrid
AU  - Muller I
AD  - Department of Medicine, Imperial College London, London, United Kingdom.
FAU - Lane, Majella E
AU  - Lane ME
AD  - University College London, School of Pharmacy, London, United Kingdom.
FAU - Kropf, Pascale
AU  - Kropf P
AD  - Department of Medicine, Imperial College London, London, United Kingdom.
FAU - Rafati, Sima
AU  - Rafati S
AUID- ORCID: http://orcid.org/0000-0002-7221-1320
AD  - Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of
      Iran, Tehran, Iran.
LA  - eng
PT  - Evaluation Studies
PT  - Journal Article
DEP - 20170713
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Costs and Cost Analysis
MH  - Female
MH  - Humans
MH  - Infant
MH  - Leishmaniasis, Cutaneous/*diagnosis
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/economics/methods
MH  - Pilot Projects
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Specimen Handling/*methods
MH  - Young Adult
EDAT- 2017/07/14 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/07/14 06:00
PHST- 2017/02/23 [received]
PHST- 2017/06/26 [accepted]
PHST- 2017/07/25 [revised]
AID - 10.1371/journal.pntd.0005750 [doi]
AID - PNTD-D-17-00256 [pii]
PST - epublish
SO  - PLoS Negl Trop Dis. 2017 Jul 13;11(7):e0005750. doi:
      10.1371/journal.pntd.0005750. eCollection 2017 Jul.