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Long non-coding RNA CCAT2 promotes cell proliferation and invasion through regulating Wnt/β-catenin signaling pathway in clear cell renal cell carcinoma.

Abstract Clear cell renal cell carcinoma (ccRCC) is a common urologic malignancy. Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) has been suggested as serving pivotal roles in tumorigenesis. However, the clinical significance and biological role of CCAT2 in ccRCC remains elusive. The purpose of this study is to identify the function of CCAT2 in ccRCC and its possible molecular mechanism. Expression of CCAT2 was analyzed in 61 ccRCC tissues and two ccRCC cell lines (786-O and ACHN) by quantitative reverse transcription polymerase chain reaction. The functional roles of CCAT2 in ccRCC were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, Transwell assay, and flow cytometric analysis. The influence of CCAT2 on tumorigenesis was monitored by in vivo mice xenograft model. The activation of Wnt/β-catenin signaling pathway was evaluated by the TOP/FOP Wnt luciferase reporter assay and western blot assay. CCAT2 expression was markedly higher in ccRCC cell lines and tissues, being positively associated with tumor size and tumor stage in ccRCC patients. Patients with higher CCAT2 expression had a markedly poorer overall survival than did patients with low CCAT2 expression. Knocking down CCAT2 expression led to reduced cell proliferation and increased apoptosis of ccRCC cells in vitro as well as the activation of Wnt/β-catenin signaling pathway, and CCAT2 overexpression remarkably enhanced these oncogenic properties. In vivo mice xenograft model also showed that knocking CCAT2 expression inhibited the growth of ccRCC xenografts. In conclusion, these results indicated that CCAT2 may play a critical role in ccRCC progression and will be further considered as a biomarker for predicting the survival of ccRCC patients and a potential therapeutic target for ccRCC intervention.
PMID
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Authors

Mayor MeshTerms
Keywords

CCAT2

Long non-coding RNA

Wnt/β-catenin signaling pathway

cell cycle

clear cell renal cell carcinoma

prognosis

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28718366
OWN - NLM
STAT- MEDLINE
DA  - 20170718
DCOM- 20170726
LR  - 20170726
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 7
DP  - 2017 Jul
TI  - Long non-coding RNA CCAT2 promotes cell proliferation and invasion through
      regulating Wnt/beta-catenin signaling pathway in clear cell renal cell carcinoma.
PG  - 1010428317711314
LID - 10.1177/1010428317711314 [doi]
AB  - Clear cell renal cell carcinoma (ccRCC) is a common urologic malignancy. Long
      non-coding RNA colon cancer-associated transcript 2 (CCAT2) has been suggested as
      serving pivotal roles in tumorigenesis. However, the clinical significance and
      biological role of CCAT2 in ccRCC remains elusive. The purpose of this study is
      to identify the function of CCAT2 in ccRCC and its possible molecular mechanism. 
      Expression of CCAT2 was analyzed in 61 ccRCC tissues and two ccRCC cell lines
      (786-O and ACHN) by quantitative reverse transcription polymerase chain reaction.
      The functional roles of CCAT2 in ccRCC were determined by
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony
      formation assay, Transwell assay, and flow cytometric analysis. The influence of 
      CCAT2 on tumorigenesis was monitored by in vivo mice xenograft model. The
      activation of Wnt/beta-catenin signaling pathway was evaluated by the TOP/FOP Wnt
      luciferase reporter assay and western blot assay. CCAT2 expression was markedly
      higher in ccRCC cell lines and tissues, being positively associated with tumor
      size and tumor stage in ccRCC patients. Patients with higher CCAT2 expression had
      a markedly poorer overall survival than did patients with low CCAT2 expression.
      Knocking down CCAT2 expression led to reduced cell proliferation and increased
      apoptosis of ccRCC cells in vitro as well as the activation of Wnt/beta-catenin
      signaling pathway, and CCAT2 overexpression remarkably enhanced these oncogenic
      properties. In vivo mice xenograft model also showed that knocking CCAT2
      expression inhibited the growth of ccRCC xenografts. In conclusion, these results
      indicated that CCAT2 may play a critical role in ccRCC progression and will be
      further considered as a biomarker for predicting the survival of ccRCC patients
      and a potential therapeutic target for ccRCC intervention.
FAU - Huang, Jian-Lin
AU  - Huang JL
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, China.
FAU - Liao, Yong
AU  - Liao Y
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, China.
FAU - Qiu, Ming-Xing
AU  - Qiu MX
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, China.
FAU - Li, Jun
AU  - Li J
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, China.
FAU - An, Yu
AU  - An Y
AD  - Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial
      People's Hospital, Chengdu, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long non-coding RNA CCAT2, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Apoptosis/genetics
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinogenesis/*genetics
MH  - Carcinoma, Renal Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - RNA, Long Noncoding/*genetics
MH  - Wnt Signaling Pathway/genetics
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - CCAT2
OT  - Long non-coding RNA
OT  - Wnt/beta-catenin signaling pathway
OT  - cell cycle
OT  - clear cell renal cell carcinoma
OT  - prognosis
EDAT- 2017/07/19 06:00
MHDA- 2017/07/27 06:00
CRDT- 2017/07/19 06:00
AID - 10.1177/1010428317711314 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jul;39(7):1010428317711314. doi: 10.1177/1010428317711314.