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Cancer-associated fibroblasts secrete FGF-1 to promote ovarian proliferation, migration, and invasion through the activation of FGF-1/FGFR4 signaling.

Abstract Ovarian cancer is the most lethal gynecologic malignancy, due to its high propensity for metastasis. Cancer-associated fibroblasts, as the dominant component of tumor microenvironment, are crucial for tumor progression. However, the mechanisms underlying the regulation of ovarian cancer cells by cancer-associated fibroblasts remain little known. Here, we first isolated cancer-associated fibroblasts from patients' ovarian tissues and found that cancer-associated fibroblasts promoted SKOV3 cells' proliferation, migration, and invasion. Fibroblast growth factor-1 was identified as a highly increased factor in cancer-associated fibroblasts compared with normal fibroblasts by quantitative reverse transcription polymerase chain reaction (~4.6-fold, p < 0.01) and ELISA assays (~4-fold, p < 0.01). High expression of fibroblast growth factor-1 in cancer-associated fibroblasts either naturally or through gene recombination led to phosphorylation of fibroblast growth factor receptor 4 in SKOV3 cells, which is followed by the activation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway and epithelial-to-mesenchymal transition-associated gene Snail1 and MMP3 expression. Moreover, treatment of SKOV3 cell with fibroblast growth factor receptor inhibitor PD173074 terminated cellular proliferation, migration, and invasion, reduced the phosphorylation level of fibroblast growth factor receptor 4, and suppressed the activation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway. In addition, the expression level of Snail1 and MMP3 was reduced, while the expression level of E-cadherin increased. These observations suggest a crucial role for cancer-associated fibroblasts and fibroblast growth factor-1/fibroblast growth factor receptor 4 signaling in the progression of ovarian cancer. Therefore, this fibroblast growth factor-1/fibroblast growth factor receptor 4 axis may become a potential target for the treatment of ovarian cancer.
PMID
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Authors

Mayor MeshTerms
Keywords

Ovarian cancer

cancer-associated fibroblasts

fibroblast growth factor-1

invasion

migration

proliferation

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28718374
OWN - NLM
STAT- MEDLINE
DA  - 20170718
DCOM- 20170726
LR  - 20170726
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 7
DP  - 2017 Jul
TI  - Cancer-associated fibroblasts secrete FGF-1 to promote ovarian proliferation,
      migration, and invasion through the activation of FGF-1/FGFR4 signaling.
PG  - 1010428317712592
LID - 10.1177/1010428317712592 [doi]
AB  - Ovarian cancer is the most lethal gynecologic malignancy, due to its high
      propensity for metastasis. Cancer-associated fibroblasts, as the dominant
      component of tumor microenvironment, are crucial for tumor progression. However, 
      the mechanisms underlying the regulation of ovarian cancer cells by
      cancer-associated fibroblasts remain little known. Here, we first isolated
      cancer-associated fibroblasts from patients' ovarian tissues and found that
      cancer-associated fibroblasts promoted SKOV3 cells' proliferation, migration, and
      invasion. Fibroblast growth factor-1 was identified as a highly increased factor 
      in cancer-associated fibroblasts compared with normal fibroblasts by quantitative
      reverse transcription polymerase chain reaction (~4.6-fold, p &lt; 0.01) and ELISA
      assays (~4-fold, p &lt; 0.01). High expression of fibroblast growth factor-1 in
      cancer-associated fibroblasts either naturally or through gene recombination led 
      to phosphorylation of fibroblast growth factor receptor 4 in SKOV3 cells, which
      is followed by the activation of mitogen-activated protein kinase/extracellular
      signal-regulated protein kinase pathway and epithelial-to-mesenchymal
      transition-associated gene Snail1 and MMP3 expression. Moreover, treatment of
      SKOV3 cell with fibroblast growth factor receptor inhibitor PD173074 terminated
      cellular proliferation, migration, and invasion, reduced the phosphorylation
      level of fibroblast growth factor receptor 4, and suppressed the activation of
      mitogen-activated protein kinase/extracellular signal-regulated protein kinase
      pathway. In addition, the expression level of Snail1 and MMP3 was reduced, while 
      the expression level of E-cadherin increased. These observations suggest a
      crucial role for cancer-associated fibroblasts and fibroblast growth
      factor-1/fibroblast growth factor receptor 4 signaling in the progression of
      ovarian cancer. Therefore, this fibroblast growth factor-1/fibroblast growth
      factor receptor 4 axis may become a potential target for the treatment of ovarian
      cancer.
FAU - Sun, Yuanzhen
AU  - Sun Y
AD  - 1 Department of Laboratory, Laiwu Maternal and Child Health Care Hospital, Laiwu,
      China.
FAU - Fan, Xiaoli
AU  - Fan X
AD  - 2 Department of Occupational Poisoning, Shandong Academy of Occupational Health
      and Occupational Medicine, Jinan, China.
FAU - Zhang, Qing
AU  - Zhang Q
AD  - 3 Department of Laboratory, Shandong Provincial Hospital, Jinan, China.
FAU - Shi, Xiaoyu
AU  - Shi X
AD  - 1 Department of Laboratory, Laiwu Maternal and Child Health Care Hospital, Laiwu,
      China.
FAU - Xu, Guangwei
AU  - Xu G
AD  - 4 Department of Laboratory, Weihaiwei People's Hospital, Weihai, China.
FAU - Zou, Cuimin
AU  - Zou C
AD  - 4 Department of Laboratory, Weihaiwei People's Hospital, Weihai, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (Cadherins)
RN  - 0 (SNAI1 protein, human)
RN  - 0 (Snail Family Transcription Factors)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - EC 2.7.10.1 (FGFR4 protein, human)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 4)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cadherins/genetics
MH  - Cancer-Associated Fibroblasts/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Fibroblast Growth Factor 1/biosynthesis/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Matrix Metalloproteinase 3/biosynthesis/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - Receptor, Fibroblast Growth Factor, Type 4/biosynthesis/*genetics
MH  - Signal Transduction
MH  - Snail Family Transcription Factors/biosynthesis/*genetics
OTO - NOTNLM
OT  - Ovarian cancer
OT  - cancer-associated fibroblasts
OT  - fibroblast growth factor-1
OT  - invasion
OT  - migration
OT  - proliferation
EDAT- 2017/07/19 06:00
MHDA- 2017/07/27 06:00
CRDT- 2017/07/19 06:00
AID - 10.1177/1010428317712592 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jul;39(7):1010428317712592. doi: 10.1177/1010428317712592.