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MicroRNA-139-5p inhibits bladder cancer proliferation and self-renewal by targeting the Bmi1 oncogene.

Abstract MiR-139-5p has been reported to be overexpressed in many types of cancers, but its role in bladder cancer has not been elucidated yet. Here, we report that miR-139-5p functions as a tumor suppressor in bladder cancer and inhibits the cancer stem cell self-renewal by targeting Bmi1 directly. We found that miR-139-5p expression was significantly downregulated in the bladder cancer specimens compared with that in adjacent normal tissues. In vitro, restoration of miR-139-5p expression significantly inhibited the proliferation of bladder cancer cells. Mechanism analysis revealed that miR-139-5p could decrease Bmi1 protein levels by binding to the 3' untranslated region of Bmi1 messenger RNA. Stem cell-related proteins such as c-MYC, NANOG, OCT4, and KLF4 and signaling pathways such as Wnt signaling were suppressed by restoration of miR-139-5p in bladder cancer cells. In addition, miR-139-5p expression also blocked self-renewal of bladder cancer stem cells by inhibiting Bmi1. In summary, our study supports that miR-139-5p acts as a tumor suppressor in bladder cancer development and suppresses cancer stem cell property of bladder cancer. Our study also suggests that miR-139-5p has the potential to be used as a therapeutic molecule for bladder cancer treatment.
PMID
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Authors

Mayor MeshTerms
Keywords

Bmi1

MiR-139-5p

bladder cancer

cancer stem cell

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start

 



PMID- 28720065
OWN - NLM
STAT- MEDLINE
DA  - 20170719
DCOM- 20170726
LR  - 20170726
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 7
DP  - 2017 Jul
TI  - MicroRNA-139-5p inhibits bladder cancer proliferation and self-renewal by
      targeting the Bmi1 oncogene.
PG  - 1010428317718414
LID - 10.1177/1010428317718414 [doi]
AB  - MiR-139-5p has been reported to be overexpressed in many types of cancers, but
      its role in bladder cancer has not been elucidated yet. Here, we report that
      miR-139-5p functions as a tumor suppressor in bladder cancer and inhibits the
      cancer stem cell self-renewal by targeting Bmi1 directly. We found that
      miR-139-5p expression was significantly downregulated in the bladder cancer
      specimens compared with that in adjacent normal tissues. In vitro, restoration of
      miR-139-5p expression significantly inhibited the proliferation of bladder cancer
      cells. Mechanism analysis revealed that miR-139-5p could decrease Bmi1 protein
      levels by binding to the 3' untranslated region of Bmi1 messenger RNA. Stem
      cell-related proteins such as c-MYC, NANOG, OCT4, and KLF4 and signaling pathways
      such as Wnt signaling were suppressed by restoration of miR-139-5p in bladder
      cancer cells. In addition, miR-139-5p expression also blocked self-renewal of
      bladder cancer stem cells by inhibiting Bmi1. In summary, our study supports that
      miR-139-5p acts as a tumor suppressor in bladder cancer development and
      suppresses cancer stem cell property of bladder cancer. Our study also suggests
      that miR-139-5p has the potential to be used as a therapeutic molecule for
      bladder cancer treatment.
FAU - Luo, Hongbo
AU  - Luo H
AD  - 1 Department of Urology, Renmin Hospital of Wuhan University, Wuhan, P.R. China.
FAU - Yang, Rui
AU  - Yang R
AD  - 1 Department of Urology, Renmin Hospital of Wuhan University, Wuhan, P.R. China.
FAU - Li, Chun
AU  - Li C
AD  - 2 School of Pharmacy, Tongji Medical College, Huazhong University of Science and 
      Technology, Wuhan, P.R. China.
FAU - Tong, Yongqing
AU  - Tong Y
AD  - 3 Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 
      P.R. China.
FAU - Fan, Lingling
AU  - Fan L
AD  - 2 School of Pharmacy, Tongji Medical College, Huazhong University of Science and 
      Technology, Wuhan, P.R. China.
FAU - Liu, Xiuheng
AU  - Liu X
AD  - 1 Department of Urology, Renmin Hospital of Wuhan University, Wuhan, P.R. China.
FAU - Xu, Chuanrui
AU  - Xu C
AD  - 2 School of Pharmacy, Tongji Medical College, Huazhong University of Science and 
      Technology, Wuhan, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
RN  - 0 (BMI1 protein, human)
RN  - 0 (MIRN139 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/*genetics
MH  - Cell Self Renewal/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Neoplastic Stem Cells/pathology
MH  - Polycomb Repressive Complex 1/*biosynthesis/genetics
MH  - Urinary Bladder Neoplasms/*genetics/pathology
MH  - Wnt Signaling Pathway/genetics
OTO - NOTNLM
OT  - Bmi1
OT  - MiR-139-5p
OT  - bladder cancer
OT  - cancer stem cell
EDAT- 2017/07/20 06:00
MHDA- 2017/07/27 06:00
CRDT- 2017/07/20 06:00
AID - 10.1177/1010428317718414 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Jul;39(7):1010428317718414. doi: 10.1177/1010428317718414.