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PMID- 28746197
OWN - NLM
STAT- MEDLINE
DA  - 20170726
DCOM- 20170807
LR  - 20170807
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Linking)
VI  - 96
IP  - 30
DP  - 2017 Jul
TI  - Role of thymic stromal lymphopoietin in the pathogenesis of lumbar disc
      degeneration.
PG  - e7516
LID - 10.1097/MD.0000000000007516 [doi]
AB  - BACKGROUND: This study aims to investigate the role of thymic stromal
      lymphopoietin (TSLP) in the pathogenesis of lumbar disc degeneration (LDD).
      METHODS: Nucleus pulposus tissues were collected from 77 LDD patients (the case
      group), in addition, normal tissues were extracted from 21 patients suffering
      from lumbar fractures (the control group). Immunohistochemistry was applied in
      order to detect TSLP positive expression. In accordance with varying
      transfection, the cells were divided into TSLP-siRNA, TSLP-siRNA + TSLPR-siRNA,
      control, blank, anti-TSLPR, and IgG groups. Western blotting was used in order to
      detect TSLP expression in tissues, and TSLP and type II collagen (COL2AL) in cell
      culture media were detected using enzyme linked immunosorbent assay (ELISA). Cell
      viability was measured using a MTT assay. Aggrecan levels were detected using
      antonopulos, and cell apoptosis was determined using flow cytometry. RESULTS:
      TSLP-positive expression was found to be significantly higher in the case group
      compared with the control group. LDD patients' Pfirrmann grades and preoperative 
      visual analogue scale (VAS) scores were associated with TSLP-positive rate. Cells
      transfected with TSLP-siRNA and TSLPR-siRNA plasmids exhibited lower TSLP and
      thymic stromal lymphopoietin receptor (TSLPR) protein expression compared with
      the control and blank groups. Compared with the control and blank groups, there
      was significantly higher cell viability, lower cell apoptosis, and higher COL2AL 
      and Aggrecan levels in the TSLP-siRNA, anti-TSLPR, and TSLP-siRNA+TSLPR-siRNA
      groups; there were significant differences between the TSLP-siRNA, anti-TSLPR,
      and TSLP-siRNA+TSLPR-siRNA groups and IgG group (all P < .05) CONCLUSION:: Our
      study provides evidence for the hypothesis that TSLP could reflect the
      histological severity of LDD, and TSLP-siRNA and, TSLPR-siRNA could inhibit
      apoptosis of nucleus pulposus cells. The evident information obtained from the
      investigation could lead the way for new therapeutic approaches regarding LDD
      treatment.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Orthopaedics, Peking University First Hospital, Beijing , P.R.
      China.
FAU - Yi, Xiao-Dong
AU  - Yi XD
FAU - Li, Chun-De
AU  - Li CD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (Aggrecans)
RN  - 0 (Biomarkers)
RN  - 0 (CRLF2 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (thymic stromal lymphopoietin)
SB  - AIM
SB  - IM
MH  - Adult
MH  - Aggrecans/metabolism
MH  - Apoptosis/physiology
MH  - Biomarkers/metabolism
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Cytokines/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Intervertebral Disc Degeneration/*metabolism/pathology
MH  - Lumbar Vertebrae/injuries/metabolism/pathology
MH  - Male
MH  - Nucleus Pulposus/*metabolism/pathology
MH  - Pain Measurement
MH  - RNA, Small Interfering
MH  - Receptors, Cytokine/genetics/metabolism
MH  - Severity of Illness Index
MH  - Spinal Fractures/metabolism/pathology
EDAT- 2017/07/27 06:00
MHDA- 2017/08/08 06:00
CRDT- 2017/07/27 06:00
AID - 10.1097/MD.0000000000007516 [doi]
AID - 00005792-201707280-00028 [pii]
PST - ppublish
SO  - Medicine (Baltimore). 2017 Jul;96(30):e7516. doi: 10.1097/MD.0000000000007516.