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Anticancer effect of Saussurea lappa extract via dual control of apoptosis and autophagy in prostate cancer cells.

Abstract To demonstrate the mechanisms of the curative effect of Saussurea lappa ethanol extract (SLE) against prostate cancer, we evaluated the effect of SLE on the induction of apoptosis and autophagy and investigated whether SLE-induced autophagy exerts a pro-survival or pro-apoptotic effect in lymph node carcinoma of the prostate (LNCaP) prostate cancer cells. SLE was prepared using 100% ethanol and added to LNCaP cells for 24 hours. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell apoptosis was evaluated by Tali assay. The expression of apoptosis-related mRNA and proteins was analyzed by quantitative real-time RT-PCR and western blotting. SLE treatment decreased the viability of LNCaP cells and increased Bax expression while suppressing the expression of pro-caspases-8/9/3, PARP, Bid, and Bcl-2, thereby inducing apoptosis in LNCaP cells. Cell proliferation related proteins, including p-Akt, androgen receptor, and prostate-specific antigen, were suppressed by SLE treatment. SLE also induced autophagy in LNCaP cells, and inhibition of autophagy enhanced the apoptosis induced by SLE treatment. These results suggest that SLE exerts anticancer effects through the induction of both cellular apoptosis and autophagy, and apoptotic cell death can be facilitated by blocking autophagy in SLE-treated LNCaP cells. Therefore, SLE might be a potential anticancer agent for the treatment of prostate cancer.
PMID
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Authors

Mayor MeshTerms

Saussurea

Keywords
Journal Title medicine
Publication Year Start




PMID- 28746210
OWN - NLM
STAT- MEDLINE
DA  - 20170726
DCOM- 20170807
LR  - 20170807
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Linking)
VI  - 96
IP  - 30
DP  - 2017 Jul
TI  - Anticancer effect of Saussurea lappa extract via dual control of apoptosis and
      autophagy in prostate cancer cells.
PG  - e7606
LID - 10.1097/MD.0000000000007606 [doi]
AB  - To demonstrate the mechanisms of the curative effect of Saussurea lappa ethanol
      extract (SLE) against prostate cancer, we evaluated the effect of SLE on the
      induction of apoptosis and autophagy and investigated whether SLE-induced
      autophagy exerts a pro-survival or pro-apoptotic effect in lymph node carcinoma
      of the prostate (LNCaP) prostate cancer cells. SLE was prepared using 100%
      ethanol and added to LNCaP cells for 24 hours. Cell viability was determined by
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell
      apoptosis was evaluated by Tali assay. The expression of apoptosis-related mRNA
      and proteins was analyzed by quantitative real-time RT-PCR and western blotting. 
      SLE treatment decreased the viability of LNCaP cells and increased Bax expression
      while suppressing the expression of pro-caspases-8/9/3, PARP, Bid, and Bcl-2,
      thereby inducing apoptosis in LNCaP cells. Cell proliferation related proteins,
      including p-Akt, androgen receptor, and prostate-specific antigen, were
      suppressed by SLE treatment. SLE also induced autophagy in LNCaP cells, and
      inhibition of autophagy enhanced the apoptosis induced by SLE treatment. These
      results suggest that SLE exerts anticancer effects through the induction of both 
      cellular apoptosis and autophagy, and apoptotic cell death can be facilitated by 
      blocking autophagy in SLE-treated LNCaP cells. Therefore, SLE might be a
      potential anticancer agent for the treatment of prostate cancer.
FAU - Tian, Xue
AU  - Tian X
AD  - aDepartment of Life Science, Gachon University, Seongnam-si bDepartment of
      Oriental Medicine Biotechnology, Kyung Hee University, Yongin-si, Korea.
FAU - Song, Hae Seong
AU  - Song HS
FAU - Cho, Young Mi
AU  - Cho YM
FAU - Park, Bongkyun
AU  - Park B
FAU - Song, Yoon-Jae
AU  - Song YJ
FAU - Jang, Sunphil
AU  - Jang S
FAU - Kang, Se Chan
AU  - Kang SC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (Androgens)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 3K9958V90M (Ethanol)
SB  - AIM
SB  - IM
MH  - Androgens/metabolism
MH  - Antineoplastic Agents, Phytogenic/chemistry/*pharmacology
MH  - Apoptosis/drug effects/physiology
MH  - Autophagy/drug effects/physiology
MH  - Carcinoma/*drug therapy/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/chemistry
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Phytotherapy
MH  - Plant Extracts/chemical synthesis/*pharmacology
MH  - Prostatic Neoplasms/*drug therapy
MH  - RNA, Messenger/metabolism
MH  - *Saussurea
EDAT- 2017/07/27 06:00
MHDA- 2017/08/08 06:00
CRDT- 2017/07/27 06:00
AID - 10.1097/MD.0000000000007606 [doi]
AID - 00005792-201707280-00041 [pii]
PST - ppublish
SO  - Medicine (Baltimore). 2017 Jul;96(30):e7606. doi: 10.1097/MD.0000000000007606.