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The methylation status and expression of human telomerase reverse transcriptase is significantly high in oral carcinogenesis.

Abstract Telomerase activity is present in most cancers and is tightly regulated by the expression of human telomerase reverse transcriptase (hTERT). Hypermethylation in the promoter region of hTERT contributes to the regulation of hTERT expression. In this study, we investigated the methylation and expression of hTERT in oral squamous cell carcinoma (OSCC), oral leukoplakia, and normal oral mucosa. Furthermore, we investigated the significance of hTERT to the clinicopathological findings of OSCC. 35 OSCC, 50 oral leukoplakia (epithelial dysplasia n = 25, squamous cell hyperplasia n = 25), and 10 normal oral mucosa samples were investigated through methylation-specific PCR. Immunohistochemistry was analyzed in 35 OSCC, 50 oral leukoplakia, and 4 normal oral mucosa samples. The methylation and expression of hTERT increased from normal oral mucosa to oral leukoplakia to OSCC. In OSCC, all samples were methylated. However, partial methylation (20%) or unmethylation (80%), but never complete methylation, was observed in normal oral mucosa. Additionally, hTERT expression correlated with cervical lymph node metastasis. These results suggested that the methylation and expression of hTERT is high in oral carcinogenesis and may play an important role in oral cancer. hTERT expression may also be predictive of cervical lymph node metastasis.
PMID
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Authors

Mayor MeshTerms
Keywords

DNA methylation

Human telomerase reverse transcriptase

oral leukoplakia

oral squamous cell carcinoma

telomerase

Journal Title apmis : acta pathologica, microbiologica, et immunologica scandinavica
Publication Year Start


 


PMID- 28766760
OWN - NLM
STAT- In-Process
DA  - 20170802
LR  - 20170807
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 125
IP  - 9
DP  - 2017 Sep
TI  - The methylation status and expression of human telomerase reverse transcriptase
      is significantly high in oral carcinogenesis.
PG  - 797-807
LID - 10.1111/apm.12723 [doi]
AB  - Telomerase activity is present in most cancers and is tightly regulated by the
      expression of human telomerase reverse transcriptase (hTERT). Hypermethylation in
      the promoter region of hTERT contributes to the regulation of hTERT expression.
      In this study, we investigated the methylation and expression of hTERT in oral
      squamous cell carcinoma (OSCC), oral leukoplakia, and normal oral mucosa.
      Furthermore, we investigated the significance of hTERT to the clinicopathological
      findings of OSCC. 35 OSCC, 50 oral leukoplakia (epithelial dysplasia n = 25,
      squamous cell hyperplasia n = 25), and 10 normal oral mucosa samples were
      investigated through methylation-specific PCR. Immunohistochemistry was analyzed 
      in 35 OSCC, 50 oral leukoplakia, and 4 normal oral mucosa samples. The
      methylation and expression of hTERT increased from normal oral mucosa to oral
      leukoplakia to OSCC. In OSCC, all samples were methylated. However, partial
      methylation (20%) or unmethylation (80%), but never complete methylation, was
      observed in normal oral mucosa. Additionally, hTERT expression correlated with
      cervical lymph node metastasis. These results suggested that the methylation and 
      expression of hTERT is high in oral carcinogenesis and may play an important role
      in oral cancer. hTERT expression may also be predictive of cervical lymph node
      metastasis.
CI  - (c) 2017 APMIS. Published by John Wiley & Sons Ltd.
FAU - Haraguchi, Kazuya
AU  - Haraguchi K
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
FAU - Yada, Naomi
AU  - Yada N
AD  - Division of Oral Pathology, Department of Health Promotion, Kyushu Dental
      University, Kitakyushu, Japan.
FAU - Sato, Shinobu
AU  - Sato S
AD  - Department of Applied Chemistry, Research Center for Bio-microsensing Technology,
      Kyushu Institute of Technology, Kitakyushu, Japan.
FAU - Habu, Manabu
AU  - Habu M
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
FAU - Hayakawa, Mana
AU  - Hayakawa M
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
FAU - Takahashi, Osamu
AU  - Takahashi O
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
FAU - Sasaguri, Masaaki
AU  - Sasaguri M
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
FAU - Takenaka, Shigeori
AU  - Takenaka S
AD  - Department of Applied Chemistry, Research Center for Bio-microsensing Technology,
      Kyushu Institute of Technology, Kitakyushu, Japan.
FAU - Yoshioka, Izumi
AU  - Yoshioka I
AD  - Division of Oral Medicine, Department of Science of Physical Functions, Kyushu
      Dental University, Kitakyushu, Japan.
FAU - Matsuo, Kou
AU  - Matsuo K
AD  - Division of Oral Pathology, Department of Health Promotion, Kyushu Dental
      University, Kitakyushu, Japan.
FAU - Tominaga, Kazuhiro
AU  - Tominaga K
AD  - Division of Oral and Maxillofacial Surgery, Department of Science of Physical
      Functions, Kyushu Dental University, Kitakyushu, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170802
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
OTO - NOTNLM
OT  - DNA methylation
OT  - Human telomerase reverse transcriptase
OT  - oral leukoplakia
OT  - oral squamous cell carcinoma
OT  - telomerase
EDAT- 2017/08/03 06:00
MHDA- 2017/08/03 06:00
CRDT- 2017/08/03 06:00
PHST- 2016/12/07 [received]
PHST- 2017/04/25 [accepted]
AID - 10.1111/apm.12723 [doi]
PST - ppublish
SO  - APMIS. 2017 Sep;125(9):797-807. doi: 10.1111/apm.12723. Epub 2017 Aug 2.