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The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals.

Abstract This protocol is an extension to: Nat. Protoc. 5, 503-515 (2010); doi: 10.1038/nprot.2009.235; published online 25 February 2010The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title nature protocols
Publication Year Start




PMID- 28771238
OWN - NLM
STAT- MEDLINE
DA  - 20170803
DCOM- 20170811
LR  - 20170811
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 12
IP  - 9
DP  - 2017 Sep
TI  - The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections 
      in humans and animals.
PG  - 1723-1732
LID - 10.1038/nprot.2017.067 [doi]
AB  - This protocol is an extension to: Nat. Protoc. 5, 503-515 (2010); doi:
      10.1038/nprot.2009.235; published online 25 February 2010The FLOTAC is a
      sensitive, accurate, and precise technique for the diagnosis of protozoan and
      helminth infections in humans and animals. However, it requires centrifugation,
      and hence might be out of reach in resource-constrained settings. As an extension
      of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC
      technique, a logical evolution of FLOTAC conceived to perform multivalent,
      qualitative, and quantitative diagnosis of helminth and protozoan infections in
      human and animal feces, and urine. This has been found to be of most use in the
      processing of large numbers of samples with rapid laboratory workup, and for
      veterinary applications directly on-farm. In addition to the Mini-FLOTAC
      apparatus, we describe the use of the Fill-FLOTAC, a closed system used to
      facilitate the performance of the first four consecutive steps of the Mini-FLOTAC
      technique: fecal sample collection and weighing, homogenization, filtration, and 
      filling of the Mini-FLOTAC chambers. Processing of an individual sample using
      this protocol requires approximately 12 min.
FAU - Cringoli, Giuseppe
AU  - Cringoli G
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples
      Federico II, CREMOPAR, Naples, Italy.
FAU - Maurelli, Maria P
AU  - Maurelli MP
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples
      Federico II, CREMOPAR, Naples, Italy.
FAU - Levecke, Bruno
AU  - Levecke B
AD  - Department of Virology, Parasitology and Immunology, Faculty of Veterinary
      Medicine, Ghent University, Ghent, Belgium.
FAU - Bosco, Antonio
AU  - Bosco A
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples
      Federico II, CREMOPAR, Naples, Italy.
FAU - Vercruysse, Jozef
AU  - Vercruysse J
AD  - Department of Virology, Parasitology and Immunology, Faculty of Veterinary
      Medicine, Ghent University, Ghent, Belgium.
FAU - Utzinger, Jurg
AU  - Utzinger J
AD  - Swiss Tropical and Public Health Institute, Basel, Switzerland.
AD  - University of Basel, Basel, Switzerland.
FAU - Rinaldi, Laura
AU  - Rinaldi L
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples
      Federico II, CREMOPAR, Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170803
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Animals
MH  - Diagnostic Techniques and Procedures/economics/*instrumentation
MH  - Feces/microbiology/parasitology
MH  - Helminthiasis/*diagnosis
MH  - Humans
MH  - Protozoan Infections/*diagnosis
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2017/08/05 06:00
MHDA- 2017/08/12 06:00
CRDT- 2017/08/04 06:00
AID - nprot.2017.067 [pii]
AID - 10.1038/nprot.2017.067 [doi]
PST - ppublish
SO  - Nat Protoc. 2017 Sep;12(9):1723-1732. doi: 10.1038/nprot.2017.067. Epub 2017 Aug 
      3.