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Evaluation of the BD MAX Enteric Parasite Panel for the detection of Cryptosporidium parvum/hominis, Giardia duodenalis and Entamoeba histolytica.

Abstract Conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. This study sought to evaluate the automated BD MAX Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/hominis, Entamoeba histolytica and Giardia duodenalis.Methodolgy. A total of 104 known positive samples (43 Cryptosporidium parvum/hominis and 61 G. duodenalis), 15 simulated samples (E. histolytica and other Entamoeba species) and 745 patient stool samples, submitted for enteric pathogen culture and microscopy, were inoculated into BD MAX EPP sample buffer tubes (SBTs). All specimens were blinded and tested within 7 days of SBT inoculation using the BD MAX EPP assay with results compared to those generated by microscopy.Results/Key findings. Combining the results from the known positive samples and anonymously tested patient samples, the sensitivity of the BD MAX EPP assay was 100 % for both Cryptosporidium spp. and G. duodenalis. Specificities of 99.7 and 98.9 % were calculated for the detection of Cryptosporidium spp. and G. duodenalis respectively. Insufficient clinical specimen data was available to determine the performance of the assay for E. histolytica detection.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title journal of medical microbiology
Publication Year Start




PMID- 28792380
OWN - NLM
STAT- MEDLINE
DA  - 20170809
DCOM- 20170830
LR  - 20170830
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 66
IP  - 8
DP  - 2017 Aug
TI  - Evaluation of the BD MAX Enteric Parasite Panel for the detection of
      Cryptosporidium parvum/hominis, Giardia duodenalis and Entamoeba histolytica.
PG  - 1118-1123
LID - 10.1099/jmm.0.000558 [doi]
AB  - PURPOSE: Conventional laboratory detection methods for gastrointestinal parasites
      are time consuming, require considerable technical expertise and may suffer from 
      poor analytical sensitivity. This study sought to evaluate the automated BD MAX
      Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/hominis,
      Entamoeba histolytica and Giardia duodenalis.Methodolgy. A total of 104 known
      positive samples (43 Cryptosporidium parvum/hominis and 61 G. duodenalis), 15
      simulated samples (E. histolytica and other Entamoeba species) and 745 patient
      stool samples, submitted for enteric pathogen culture and microscopy, were
      inoculated into BD MAX EPP sample buffer tubes (SBTs). All specimens were blinded
      and tested within 7 days of SBT inoculation using the BD MAX EPP assay with
      results compared to those generated by microscopy.Results/Key findings. Combining
      the results from the known positive samples and anonymously tested patient
      samples, the sensitivity of the BD MAX EPP assay was 100 % for both
      Cryptosporidium spp. and G. duodenalis. Specificities of 99.7 and 98.9 % were
      calculated for the detection of Cryptosporidium spp. and G. duodenalis
      respectively. Insufficient clinical specimen data was available to determine the 
      performance of the assay for E. histolytica detection. CONCLUSIONS: The findings 
      of this study indicate that the BD MAX EPP is suitable for the detection of
      Cryptosporidium parvum/hominis and G. duodenalis from clinical specimens with
      reduced hands-on time and complexity compared to microscopy. Results for the
      detection of E. histolytica were promising although further work is required to
      evaluate the assay for the detection of this pathogen.
FAU - Perry, Michael D
AU  - Perry MD
AD  - Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath
      Park, Cardiff, UK.
FAU - Corden, Sally A
AU  - Corden SA
AD  - Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath
      Park, Cardiff, UK.
FAU - Lewis White, P
AU  - Lewis White P
AD  - Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath
      Park, Cardiff, UK.
LA  - eng
PT  - Evaluation Studies
PT  - Journal Article
DEP - 20170810
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Cryptosporidiosis/*parasitology
MH  - Cryptosporidium parvum/genetics/*isolation & purification
MH  - DNA, Protozoan/genetics
MH  - Entamoeba histolytica/genetics/*isolation & purification
MH  - Entamoebiasis/*parasitology
MH  - Feces/parasitology
MH  - Giardia lamblia/genetics/*isolation & purification
MH  - Giardiasis/*parasitology
MH  - Humans
MH  - Intestinal Diseases, Parasitic/parasitology
MH  - Nucleic Acid Amplification Techniques/*methods
EDAT- 2017/08/10 06:00
MHDA- 2017/08/31 06:00
CRDT- 2017/08/10 06:00
AID - 10.1099/jmm.0.000558 [doi]
PST - ppublish
SO  - J Med Microbiol. 2017 Aug;66(8):1118-1123. doi: 10.1099/jmm.0.000558. Epub 2017
      Aug 10.