PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Combined antioxidant effects of Neem extract, bacteria, red blood cells and Lysozyme: possible relation to periodontal disease.

Abstract The common usage of chewing sticks prepared from Neem tree (Azadirachta indica) in India suggests its potential efficacy in periodontal diseases. The objective of this study is to explore the antibacterial effects of Neem leaf extract on the periodontophatic bacteria Porphyromonas gingivalis and Fusobacterium nucleatum, and its antioxidant capacities alone and in combination with bacteria and polycationic peptides that may be at the site of inflammation.
PMID
Related Publications

NOX1/2 activation in human gingival fibroblasts by Fusobacterium nucleatum facilitates attachment of Porphyromonas gingivalis.

Green tea catechins potentiate the effect of antibiotics and modulate adherence and gene expression in Porphyromonas gingivalis.

The inhibiting effect of aqueous Azadirachta indica (Neem) extract upon bacterial properties influencing in vitro plaque formation.

Antibacterial Efficacy of Neem (Azadirachta indica) Extract against Enterococcus faecalis: An in vitro Study.

Effect of neem (Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms.

Authors

Mayor MeshTerms
Keywords

Inflammation

Plants

Polyphenols

Porphyromonas gingivalis

Journal Title bmc complementary and alternative medicine
Publication Year Start




PMID- 28797303
OWN - NLM
STAT- In-Process
DA  - 20170811
LR  - 20170811
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Aug 10
TI  - Combined antioxidant effects of Neem extract, bacteria, red blood cells and
      Lysozyme: possible relation to periodontal disease.
PG  - 399
LID - 10.1186/s12906-017-1900-3 [doi]
AB  - BACKGROUND: The common usage of chewing sticks prepared from Neem tree
      (Azadirachta indica) in India suggests its potential efficacy in periodontal
      diseases. The objective of this study is to explore the antibacterial effects of 
      Neem leaf extract on the periodontophatic bacteria Porphyromonas gingivalis and
      Fusobacterium nucleatum, and its antioxidant capacities alone and in combination 
      with bacteria and polycationic peptides that may be at the site of inflammation. 
      METHODS: Neem leaf extract was prepared by ethanol extraction. The growth
      kinetics of P. gingivalis and F. nucleatum under anaerobic conditions in the
      presence of Neem leaf extract were measured. Broth microdilution test was used to
      determine the Minimal Inhibitory Concentration (MIC) of Neem leaf extract against
      each bacterial strain. The effect of Neem leaf extract on the coaggregation of
      the bacteria was assessed by a visual semi-quantitative assay. The antioxidant
      capacities of Neem leaf extract alone and in combination with bacteria, with the 
      addition of red blood cells or the polycationic peptides chlorhexidine and
      lisozyme, were determined using a chemiluminescence assay. RESULTS: Neem leaf
      extract showed prominent dose-dependent antibacterial activity against P.
      gingivalis, however, had no effect on the growth of F. nucleatum nor on the
      coaggregation of the two bacteria. Yet, it showed intense antioxidant activity,
      which was amplified following adherence to bacteria and with the addition of red 
      blood cells or the polycationic peptides. CONCLUSIONS: Neem leaf extract,
      containing polyphenols that adhere to oral surfaces, have the potential to
      provide long-lasting antibacterial as well as synergic antioxidant activities
      when in complex with bacteria, red blood cells and lisozyme. Thus, it might be
      especially effective in periodontal diseases.
FAU - Heyman, Leali
AU  - Heyman L
AD  - Department of Prosthodontics, Hebrew University-Hadassah Faculty of Dental
      Medicine, P.O.B. 12272, 91120, Jerusalem, Israel.
FAU - Houri-Haddad, Yael
AU  - Houri-Haddad Y
AD  - Department of Prosthodontics, Hebrew University-Hadassah Faculty of Dental
      Medicine, P.O.B. 12272, 91120, Jerusalem, Israel.
FAU - Heyman, Samuel N
AU  - Heyman SN
AD  - Department of Medicine, Hadassah-Hebrew University Hospital, Mount Scopus,
      Jerusalem, Israel.
FAU - Ginsburg, Isaac
AU  - Ginsburg I
AD  - Institute for Dental Research, Hebrew University-Hadassah Faculty of Dental
      Medicine, Jerusalem, Israel.
FAU - Gleitman, Yossi
AU  - Gleitman Y
AD  - Department of Prosthodontics, Hebrew University-Hadassah Faculty of Dental
      Medicine, P.O.B. 12272, 91120, Jerusalem, Israel.
FAU - Feuerstein, Osnat
AU  - Feuerstein O
AUID- ORCID: http://orcid.org/0000-0003-1820-8291
AD  - Department of Prosthodontics, Hebrew University-Hadassah Faculty of Dental
      Medicine, P.O.B. 12272, 91120, Jerusalem, Israel. [email protected]
LA  - eng
PT  - Journal Article
DEP - 20170810
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
OTO - NOTNLM
OT  - Inflammation
OT  - Plants
OT  - Polyphenols
OT  - Porphyromonas gingivalis
EDAT- 2017/08/12 06:00
MHDA- 2017/08/12 06:00
CRDT- 2017/08/12 06:00
PHST- 2016/03/31 [received]
PHST- 2017/08/02 [accepted]
AID - 10.1186/s12906-017-1900-3 [doi]
AID - 10.1186/s12906-017-1900-3 [pii]
PST - epublish
SO  - BMC Complement Altern Med. 2017 Aug 10;17(1):399. doi: 10.1186/s12906-017-1900-3.