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Anthelminthic properties of Methylene chloride-methanol (1:1) extracts of two Cameroonians medicinal plants on Heligmosomoides bakeri (Nematoda: Heligmosomatidea).

Abstract The resistance of some medico-veterinary parasite strains as well as the unavailability and toxicity of synthetic anthelminthics on humans, animals and the impacts of their residues in the environment have pushed scientists to turn to plants with anthelminthic properties. Hence, the aim of this work was to contribute to the fight against helminths of medical and veterinary importance in general, and also to clear the environment of their free living stages.
PMID
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Authors

Mayor MeshTerms
Keywords

Additive effect

Annona senegalensis

Heligmosomoides bakeri

Nauclea latifolia

Journal Title bmc complementary and alternative medicine
Publication Year Start




PMID- 28800757
OWN - NLM
STAT- MEDLINE
DA  - 20170812
DCOM- 20170901
LR  - 20170901
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Aug 11
TI  - Anthelminthic properties of Methylene chloride-methanol (1:1) extracts of two
      Cameroonians medicinal plants on Heligmosomoides bakeri (Nematoda:
      Heligmosomatidea).
PG  - 400
LID - 10.1186/s12906-017-1908-8 [doi]
AB  - BACKGROUND: The resistance of some medico-veterinary parasite strains as well as 
      the unavailability and toxicity of synthetic anthelminthics on humans, animals
      and the impacts of their residues in the environment have pushed scientists to
      turn to plants with anthelminthic properties. Hence, the aim of this work was to 
      contribute to the fight against helminths of medical and veterinary importance in
      general, and also to clear the environment of their free living stages. METHODS: 
      Fresh eggs of Heligmosomoides bakeri were obtained from the faeces of
      experimentally infected mice. L1 and L2 larval stages were obtained after 48 and 
      72 h of coproculture respectively. Methylene Chloride-Methanol (1:1) extracts of 
      Annona senegalensis and Nauclea latifolia were diluted in DMSO or Tween 80 to
      prepare the following concentrations: 625, 1250, 2500, 3750 and 5000 mug/ml. The 
      effects of extract solutions were evaluated on the embryonation of eggs, egg
      hatching and on L1 and L2 survival after 48, 10 and 24 h of incubation. Negative 
      controls were 1.5% DMSO, 4% Tween 80 and a mixture of these solvents. The TLC was
      carried out and the profiles of secondary metabolites were made. RESULTS:
      Negative controls had no effect on the embryonation, eggs hatching and on larval 
      mortality. However, it was found that, the extracts affected the free living
      stages of H. bakeri in a concentration-dependant manner. At the highest
      concentration (5000 mug/ml), the rate of inhibition of embryonation obtained were
      20.80%, 38.15% and 84.83% for Methylene Chloride-Methanol of Annona senegalensis 
      (MCM As), Nauclea latifolia (MCM Nl) extracts and mixture of Annona senegalensis 
      and Nauclea latifolia (MCM As-Nl) extract respectively. For egg hatch, the
      inhibition rate was 16.10%, 46.24% and 87.07% for the above three extracts
      respectively at the same concentration of 5000 mug/ml. On L1 and L2 larval stages
      after 24 h of exposure to extracts, the mortality rates of 100%, 54.76% and
      96.77% against 98%, 51.44% and 100% were obtained for MCM As, MCM Nl and MCM
      As-Nl respectively at the highest concentration. The Methylene Chloride-Methanol 
      of A.senegalensis, N. latifolia extracts showed the presence of alkaloids except 
      in N. latifolia extract, flavonoids, sterols, triterpens, tanins, polyphenols,
      anthraquinons, saponins and terpenoids. CONCLUSION: These findings suggest that, 
      the mixture of the two plant extracts showed an additive (synergetic effect)
      ovicidal effect and a slight larval mortality on L1 as compared to the effect of 
      MCM As extract alone. These effects were due to the presence ao secondary
      metabolites identifies in the plant extracts. Thus, they may be used as possible 
      <<disinfectants>> for soil transmitted nematodes.
FAU - Ngouateu Teufack, Sergine Errole
AU  - Ngouateu Teufack SE
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
FAU - NMbogning Tayo, Gertrude
AU  - NMbogning Tayo G
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
FAU - Ngangout Alidou, Marc
AU  - Ngangout Alidou M
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
FAU - Yondo, Jeannette
AU  - Yondo J
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
FAU - Djiomene, Amely Frankline
AU  - Djiomene AF
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
FAU - Wabo Pone, Josue
AU  - Wabo Pone J
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
      [email protected]
FAU - Mbida, Fauily Mpoame
AU  - Mbida FM
AD  - Research Unit of Biology and Applied Ecology, Department of Animal Biology,
      Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon.
LA  - eng
PT  - Journal Article
DEP - 20170811
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Anthelmintics)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Annona/*chemistry
MH  - Anthelmintics/*pharmacology
MH  - Cameroon
MH  - Drug Synergism
MH  - Larva/*drug effects
MH  - Life Cycle Stages/drug effects
MH  - Medicine, African Traditional
MH  - Mice
MH  - Nematoda/*drug effects/growth & development
MH  - Nematode Infections/*parasitology
MH  - Phytochemicals/analysis/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Rubiaceae/*chemistry
PMC - PMC5553925
OTO - NOTNLM
OT  - Additive effect
OT  - Annona senegalensis
OT  - Heligmosomoides bakeri
OT  - Nauclea latifolia
EDAT- 2017/08/13 06:00
MHDA- 2017/09/02 06:00
CRDT- 2017/08/13 06:00
PHST- 2017/01/24 [received]
PHST- 2017/08/02 [accepted]
AID - 10.1186/s12906-017-1908-8 [doi]
AID - 10.1186/s12906-017-1908-8 [pii]
PST - epublish
SO  - BMC Complement Altern Med. 2017 Aug 11;17(1):400. doi: 10.1186/s12906-017-1908-8.