PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Sema3A Reduces Sprouting of Adult Rod Photoreceptors In Vitro.

Abstract Rod photoreceptor terminals respond to retinal injury with retraction and sprouting. Since the guidance cue Semaphorin3A (Sema3A) is observed in the retina after injury, we asked whether Sema3A contributes to structural plasticity in rod photoreceptors.
PMID
Related Publications

Mislocalized opsin and cAMP signaling: a mechanism for sprouting by rod cells in retinal degeneration.

The nitric oxide-cGMP signaling pathway differentially regulates presynaptic structural plasticity in cone and rod cells.

RhoA inactivation prevents photoreceptor axon retraction in an in vitro model of acute retinal detachment.

RhoA and its role in synaptic structural plasticity of isolated salamander photoreceptors.

LIM Kinase, a Newly Identified Regulator of Presynaptic Remodeling by Rod Photoreceptors After Injury.

Authors

Mayor MeshTerms

Disease Models, Animal

Keywords
Journal Title investigative ophthalmology & visual science
Publication Year Start




PMID- 28806446
OWN - NLM
STAT- MEDLINE
DA  - 20170814
DCOM- 20170821
LR  - 20170821
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 10
DP  - 2017 Aug 01
TI  - Sema3A Reduces Sprouting of Adult Rod Photoreceptors In Vitro.
PG  - 4038-4051
LID - 10.1167/iovs.16-21075 [doi]
AB  - Purpose: Rod photoreceptor terminals respond to retinal injury with retraction
      and sprouting. Since the guidance cue Semaphorin3A (Sema3A) is observed in the
      retina after injury, we asked whether Sema3A contributes to structural plasticity
      in rod photoreceptors. Methods: We used Western blots and alkaline phosphatase
      (AP)-tagged neuropilin-1 (NPN-1) to detect the expression of Sema3A in an
      organotypic model of porcine retinal detachment. We then examined Sema3A binding 
      to cultured salamander rod photoreceptors using AP-tagged Sema3A. For functional 
      analysis, we used a microspritzer to apply a gradient of Sema3A-Fc to isolated
      salamander rod photoreceptors over 24 hours. Results: Sema3A protein was
      biochemically detected in porcine retinal explants in the retina 7, 24, and 72
      hours after detachment. In sections, NPN-1 receptor was bound to the inner and
      outer retina. For isolated rod photoreceptors, Sema3A localized to synaptic
      terminals and to neuritic processes after 1 week in vitro. In microspritzed rod
      photoreceptors, process initiation occurred away from high concentrations of
      Sema3A. Sema3A significantly decreased the number of processes formed by rod
      photoreceptors although the average length of processes was not affected. The
      cellular orientation of rod photoreceptors relative to the microspritzer also
      significantly changed over time; this effect was reduced with the Sema3A
      inhibitor, xanthofulvin. Conclusion: Sema3A is expressed in the retina after
      detachment, binds to rod photoreceptors, affects cell orientation, and reduces
      photoreceptor process initiation in vitro. Our results suggest that Sema3A
      contributes to axonal retraction in retinal injury, whereas rod neuritic
      sprouting and regenerative synaptogenesis may require a reduction in semaphorin
      signaling.
FAU - Kung, Frank
AU  - Kung F
AD  - Joint Program in Biomedical Engineering, Rutgers University, Graduate School of
      Biomedical Sciences, New Jersey Institute of Technology, Newark, United States.
FAU - Wang, Weiwei
AU  - Wang W
AD  - Department of Pharmacology, Physiology, and Neuroscience, Rutgers University, New
      Jersey Medical School, Newark, New Jersey, United States.
FAU - Tran, Tracy S
AU  - Tran TS
AD  - Department of Biological Sciences, Rutgers University, Newark College of Arts and
      Sciences, Newark, New Jersey, United States.
FAU - Townes-Anderson, Ellen
AU  - Townes-Anderson E
AD  - Department of Pharmacology, Physiology, and Neuroscience, Rutgers University, New
      Jersey Medical School, Newark, New Jersey, United States.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Semaphorin-3A)
RN  - 144713-63-3 (Neuropilin-1)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Ambystoma
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - *Disease Models, Animal
MH  - Neurites/physiology
MH  - Neuronal Plasticity/*physiology
MH  - Neuropilin-1/metabolism
MH  - Organ Culture Techniques
MH  - Presynaptic Terminals
MH  - Retinal Detachment/*metabolism
MH  - Retinal Neurons/*physiology
MH  - Retinal Rod Photoreceptor Cells/*metabolism
MH  - Semaphorin-3A/*metabolism
MH  - Swine
PMC - PMC5555408
EDAT- 2017/08/15 06:00
MHDA- 2017/08/22 06:00
CRDT- 2017/08/15 06:00
AID - 2648897 [pii]
AID - 10.1167/iovs.16-21075 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4038-4051. doi:
      10.1167/iovs.16-21075.