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In vitro antiplasmodial activity and prophylactic potentials of extract and fractions of Trema orientalis (Linn.) stem bark.

Abstract Trema orientalis (T. orientalis Linn) has been used in the management of malaria in the western part of Nigeria and despite its application in ethnomedicine, there is dearth of scientific evidence to justify the acclaimed prophylactic antimalarial usage of the plant. The aim of this study is to assess the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the methanol extract of the stem bark of T. orientalis and its fractions as a prophylactic regimen for malaria prevention. Also, the antimicrobial activities of the extract and the fractions were investigated.
PMID
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Authors

Mayor MeshTerms

Phytotherapy

Trema

Keywords

Chloroquine

Ferriprotoporphyrin (ix)

Hemin

Hemozoin

Plasmodium bergei

Sulfadoxine-pyrimetamine

Trema orientalis

Journal Title bmc complementary and alternative medicine
Publication Year Start




PMID- 28810849
OWN - NLM
STAT- MEDLINE
DA  - 20170816
DCOM- 20170901
LR  - 20170901
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Aug 15
TI  - In vitro antiplasmodial activity and prophylactic potentials of extract and
      fractions of Trema orientalis (Linn.) stem bark.
PG  - 407
LID - 10.1186/s12906-017-1914-x [doi]
AB  - BACKGROUND: Trema orientalis (T. orientalis Linn) has been used in the management
      of malaria in the western part of Nigeria and despite its application in
      ethnomedicine, there is dearth of scientific evidence to justify the acclaimed
      prophylactic antimalarial usage of the plant. The aim of this study is to assess 
      the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the
      methanol extract of the stem bark of T. orientalis and its fractions as a
      prophylactic regimen for malaria prevention. Also, the antimicrobial activities
      of the extract and the fractions were investigated. METHOD: Vacuum liquid
      chromatography was used to obtain dichloromethane, ethylacetate and methanol
      fractions from the methanol extract of T. orientalis. The fractions were tested
      for their prophylactic and cell-free antimalarial activity using murine models
      and beta-hematin formation assay respectively. Disc diffusion method was used to 
      determine the antibacterial activity of the extract and its fractions against
      both Gram-positive and Gram-negative bacteria. RESULTS: In the prophylactic
      experiment, dichloromethane (DCMF), methanol fraction (MF) and extract (ME) (in
      this order) showed significant chemopreventive effects against P. berghei
      invasion of the red blood cells when compared with both Sulfadoxine-Pyrimethamine
      (SP) and untreated controls. Results of the in vitro study showed that the DCMF
      had the highest effect in preventing the formation of beta-hematin when compared 
      with other fractions. The DCMF also had the highest percentage inhibition of
      beta-hematin formation when compared with chloroquine. The extract and fractions 
      showed a concentration dependent antibacterial activity. Methanol extract had a
      pronounced inhibitory effect on Enterobacter cloaca ATCC 13047 and Enterococcus
      faecalis ATCC 29212. Serratia mercescens ATCC 9986 and Pseudomonas aeruginosa
      ATCC 19582 were the most susceptible bacteria. CONCLUSION: The results obtained
      showed that both extract and fractions of T. orientalis possessed antiplasmodial 
      and antimicrobial activity.
FAU - Olanlokun, John Oludele
AU  - Olanlokun JO
AD  - Laboratories for Biomembrane Research and Biotechnology, Department of
      Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University 
      of Ibadan, Ibadan, Nigeria. [email protected]
FAU - David, Oluwole Moses
AU  - David OM
AD  - Department of Microbiology, Faculty of Science, Ekiti State University,
      Ado-Ekiti, Nigeria.
FAU - Afolayan, Anthony Jide
AU  - Afolayan AJ
AD  - Medicinal Plants and Economic Development Research Centre, Botany Department,
      University of Fort Hare, Alice, 5700, South Africa.
LA  - eng
PT  - Journal Article
DEP - 20170815
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antimalarials)
RN  - 0 (Hemeproteins)
RN  - 0 (Plant Extracts)
RN  - 39404-00-7 (hemozoin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Antimalarials/pharmacology/*therapeutic use
MH  - Gram-Negative Bacteria/drug effects
MH  - Gram-Positive Bacteria/drug effects
MH  - Hemeproteins/metabolism
MH  - Malaria/blood/parasitology/*prevention & control
MH  - Male
MH  - Mice
MH  - *Phytotherapy
MH  - Plant Bark
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Plant Stems
MH  - Plasmodium berghei/*drug effects/growth & development
MH  - *Trema
PMC - PMC5558712
OTO - NOTNLM
OT  - Chloroquine
OT  - Ferriprotoporphyrin (ix)
OT  - Hemin
OT  - Hemozoin
OT  - Plasmodium bergei
OT  - Sulfadoxine-pyrimetamine
OT  - Trema orientalis
EDAT- 2017/08/16 06:00
MHDA- 2017/09/02 06:00
CRDT- 2017/08/17 06:00
PHST- 2017/03/16 [received]
PHST- 2017/08/07 [accepted]
AID - 10.1186/s12906-017-1914-x [doi]
AID - 10.1186/s12906-017-1914-x [pii]
PST - epublish
SO  - BMC Complement Altern Med. 2017 Aug 15;17(1):407. doi: 10.1186/s12906-017-1914-x.