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Mycetoma laboratory diagnosis: Review article.

Abstract Mycetoma is a unique neglected tropical disease caused by a substantial number of microorganisms of fungal or bacterial origins. Identification of the causative organism and the disease extension are the first steps in the management of the affected patients and predicting disease treatment outcome and prognosis. Different laboratory-based diagnostic tools and techniques were developed over the years to determine and identify the causative agents. These include direct microscopy and cytological, histopathological, and immunohistochemical techniques in addition to the classical grain culture. More recently, various molecular-based techniques have joined the mycetoma diagnostic armamentarium. The available mycetoma diagnostic techniques are of various specificity and sensitivity rates. Most are invasive, time consuming, and operator dependent, and a combination of them is required to reach a diagnosis. In addition, they need a well-equipped laboratory and are therefore not field friendly. This review aims to provide an update on the laboratory investigations used in the diagnosis of mycetoma. It further aims to assist practising health professionals dealing with mycetoma by outlining the guidelines developed by the Mycetoma Research Centre, University of Khartoum, WHO collaborating centre on mycetoma following a cumulative experience of managing more than 7,700 mycetoma patients.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos neglected tropical diseases
Publication Year Start




PMID- 28837657
OWN - NLM
STAT- MEDLINE
DA  - 20170824
DCOM- 20170905
LR  - 20170906
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Linking)
VI  - 11
IP  - 8
DP  - 2017 Aug
TI  - Mycetoma laboratory diagnosis: Review article.
PG  - e0005638
LID - 10.1371/journal.pntd.0005638 [doi]
AB  - Mycetoma is a unique neglected tropical disease caused by a substantial number of
      microorganisms of fungal or bacterial origins. Identification of the causative
      organism and the disease extension are the first steps in the management of the
      affected patients and predicting disease treatment outcome and prognosis.
      Different laboratory-based diagnostic tools and techniques were developed over
      the years to determine and identify the causative agents. These include direct
      microscopy and cytological, histopathological, and immunohistochemical techniques
      in addition to the classical grain culture. More recently, various
      molecular-based techniques have joined the mycetoma diagnostic armamentarium. The
      available mycetoma diagnostic techniques are of various specificity and
      sensitivity rates. Most are invasive, time consuming, and operator dependent, and
      a combination of them is required to reach a diagnosis. In addition, they need a 
      well-equipped laboratory and are therefore not field friendly. This review aims
      to provide an update on the laboratory investigations used in the diagnosis of
      mycetoma. It further aims to assist practising health professionals dealing with 
      mycetoma by outlining the guidelines developed by the Mycetoma Research Centre,
      University of Khartoum, WHO collaborating centre on mycetoma following a
      cumulative experience of managing more than 7,700 mycetoma patients.
FAU - Ahmed, Amel Altayeb
AU  - Ahmed AA
AD  - The Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan.
FAU - van de Sande, Wendy
AU  - van de Sande W
AD  - Department of Medical Microbiology & Infectious Diseases, Erasmus MC, University 
      of Rotterdam, Rotterdam, the Netherlands.
FAU - Fahal, Ahmed Hassan
AU  - Fahal AH
AUID- ORCID: http://orcid.org/0000-0003-4922-7321
AD  - The Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20170824
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antifungal Agents)
SB  - IM
MH  - Anti-Bacterial Agents/therapeutic use
MH  - Antifungal Agents/therapeutic use
MH  - Bacteria/*isolation & purification
MH  - Clinical Laboratory Techniques
MH  - Fungi/*isolation & purification
MH  - Humans
MH  - Mycetoma/*diagnosis/drug therapy/*microbiology
MH  - Practice Guidelines as Topic
MH  - Treatment Outcome
MH  - Tropical Medicine
EDAT- 2017/08/25 06:00
MHDA- 2017/09/07 06:00
CRDT- 2017/08/25 06:00
AID - 10.1371/journal.pntd.0005638 [doi]
AID - PNTD-D-17-00027 [pii]
PST - epublish
SO  - PLoS Negl Trop Dis. 2017 Aug 24;11(8):e0005638. doi:
      10.1371/journal.pntd.0005638. eCollection 2017 Aug.