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PMID- 28837732
OWN - NLM
STAT- MEDLINE
DA  - 20170824
DCOM- 20170904
LR  - 20170904
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 10
DP  - 2017 Aug 01
TI  - Candida Species From Eye Infections: Drug Susceptibility, Virulence Factors, and 
      Molecular Characterization.
PG  - 4201-4209
LID - 10.1167/iovs.17-22003 [doi]
AB  - Purpose: To determine the type of Candida species in ocular infections and to
      investigate the relationship of antifungal susceptibility profile to virulence
      factors. Methods: Fifty isolates of yeast-like fungi from patients with
      keratitis, endophthalmitis, and orbital cellulitis were identified by Vitek-2
      compact system and DNA sequencing of ITS1-5.8S-ITS2 regions of the rRNA gene,
      followed by phylogenetic analysis for phenotypic and genotypic identification,
      respectively. Minimum inhibitory concentration of six antifungal drugs was
      determined by E test/microbroth dilution methods. Phenotypic and genotypic
      methods were used to determine the virulence factors. Results: Phylogenetic
      analysis showed the clustering of all isolates into eight distinct groups with a 
      major cluster formed Candida parapsilosis (n = 21), which was the most common
      species by both Vitek 2 and DNA sequencing. Using chi2 test no significant
      difference was noted between the techniques except that Vitek 2 did not identify 
      C. viswanathii, C. orthopsilosis, and two non-Candida genera. Of 43 tested
      Candida isolates high susceptibility to amphotericin B (39/43, 90.6%) and
      natamycin (43/43, 100%) was noted. While none of the isolates produced coagulase,
      all produced esterase and catalase. The potential to form biofilm was detected in
      23/43 (53.4%) isolates. Distribution of virulence factors by heat map analysis
      showed difference in metabolic activity of biofilm producers from nonbiofilm
      producers. Conclusions: Identified by Vitek 2 and DNA sequencing methods C.
      parapsilosis was the most common species associated with eye infections.
      Irrespective of the virulence factors elaborated, the Candida isolates were
      susceptible to commonly used antifungal drugs such as amphotericin B and
      natamycin.
FAU - Ranjith, Konduri
AU  - Ranjith K
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
AD  - Research scholar, Manipal University, Madhav Nagar, Udipi, Karnataka, India.
FAU - Sontam, Bhavani
AU  - Sontam B
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
FAU - Sharma, Savitri
AU  - Sharma S
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
FAU - Joseph, Joveeta
AU  - Joseph J
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
FAU - Chathoth, Kanchana N
AU  - Chathoth KN
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
FAU - Sama, Kalyana C
AU  - Sama KC
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
FAU - Murthy, Somasheila I
AU  - Murthy SI
AD  - Tej Kohli Cornea Institute, L. V. Prasad Eye Institute, Kallam Anji Reddy campus,
      Hyderabad, India.
FAU - Shivaji, Sisinthy
AU  - Shivaji S
AD  - Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye
      Institute, Kallam Anji Reddy campus, Hyderabad, India.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antifungal Agents)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Virulence Factors)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - 8O0C852CPO (Natamycin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amphotericin B/pharmacology
MH  - Antifungal Agents/*pharmacology
MH  - Biofilms/growth & development
MH  - *Candida/drug effects/genetics/isolation & purification/metabolism
MH  - Candidiasis/*microbiology
MH  - Child, Preschool
MH  - Endophthalmitis/microbiology
MH  - Eye Infections, Fungal/*microbiology
MH  - Female
MH  - Humans
MH  - Keratitis/microbiology
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Natamycin/pharmacology
MH  - Orbital Cellulitis/microbiology
MH  - RNA, Fungal/genetics
MH  - RNA, Ribosomal/genetics
MH  - Sequence Analysis, DNA
MH  - Virulence Factors/*metabolism
MH  - Young Adult
EDAT- 2017/08/25 06:00
MHDA- 2017/09/05 06:00
CRDT- 2017/08/25 06:00
AID - 2652421 [pii]
AID - 10.1167/iovs.17-22003 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4201-4209. doi:
      10.1167/iovs.17-22003.