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Efficacy of a Fixed Combination of Tetracycline, Chloramphenicol, and Colistimethate Sodium for Treatment of Candida albicans Keratitis.

Abstract To evaluate the antifungal activity of a fixed antibiotic combination (AC) containing tetracycline (TET), chloramphenicol (CAF), and colistimethate sodium (CS).
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title investigative ophthalmology & visual science
Publication Year Start




PMID- 28846777
OWN - NLM
STAT- MEDLINE
DA  - 20170828
DCOM- 20170904
LR  - 20170904
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 10
DP  - 2017 Aug 01
TI  - Efficacy of a Fixed Combination of Tetracycline, Chloramphenicol, and
      Colistimethate Sodium for Treatment of Candida albicans Keratitis.
PG  - 4292-4298
LID - 10.1167/iovs.17-22047 [doi]
AB  - Purpose: To evaluate the antifungal activity of a fixed antibiotic combination
      (AC) containing tetracycline (TET), chloramphenicol (CAF), and colistimethate
      sodium (CS). Methods: In vitro: Candida ATCC and clinical strains were used. The 
      minimum inhibitory concentrations (MICs) of AC and of each antibiotic were
      determined. Fluconazole (FLC) was tested for comparison. Time-killing curves of
      selected strains were performed. Ex vivo keratitis: corneas were injected
      intrastromally with the selected strains. After the injection, corneas were
      divided into groups of treatments: AC, FLC, or saline. Then, the tissues were
      analyzed for colony-forming units per gram (CFU/g). Propidium iodide (PI) and
      MitoTracker (MTR) staining were used to investigate the mode of action. Results: 
      Values of MIC required to inhibit the growth of 90% of organisms for the
      antibiotics alone were higher than FLC. However, their activity was enhanced when
      used in combination against Candida yeasts. Time-killing curves showed that at 24
      hours, AC reduced the load of both strains of approximately 1 Log10 CFU/g
      compared with the initial inoculum (P < 0.0001). This effect was also significant
      versus FLC. In ex vivo, AC was effective in decreasing the loads of both strains 
      by 4 Log10 CFU/g with respect to the control. Moreover, it showed higher activity
      than FLC against Candida albicans ATCC 10231 (1 Log10 CFU/g, P < 0.01 versus
      control). PI staining demonstrated that CS changed the membrane's permeability,
      whereas MTR staining demonstrated that TET or CAF altered mitochondrial function.
      The cells treated with AC and stained showed both effects. Conclusions: In this
      study, AC showed antifungal efficacy versus Candida spp.; this activity can be
      due to the synergistic effects of antibiotics in it.
FAU - Blanco, Anna R
AU  - Blanco AR
AD  - SIFI SpA, Aci S. Antonio, Catania, Italy.
FAU - Nostro, Antonia
AU  - Nostro A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences,
      University of Messina, Polo Annunziata, Messina, Italy.
FAU - D'Angelo, Valeria
AU  - D'Angelo V
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences,
      University of Messina, Polo Annunziata, Messina, Italy.
FAU - D'Arrigo, Manuela
AU  - D'Arrigo M
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences,
      University of Messina, Polo Annunziata, Messina, Italy.
FAU - Mazzone, Maria G
AU  - Mazzone MG
AD  - SIFI SpA, Aci S. Antonio, Catania, Italy.
FAU - Marino, Andreana
AU  - Marino A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences,
      University of Messina, Polo Annunziata, Messina, Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Drug Combinations)
RN  - 0 (Ophthalmic Solutions)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - DL2R53P963 (colistinmethanesulfonic acid)
RN  - F8VB5M810T (Tetracycline)
RN  - Z67X93HJG1 (Colistin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*therapeutic use
MH  - Candida albicans/*drug effects/isolation & purification
MH  - Candidiasis/*drug therapy/microbiology
MH  - Chloramphenicol/*therapeutic use
MH  - Colistin/*analogs & derivatives/therapeutic use
MH  - Colony Count, Microbial
MH  - Corneal Ulcer/*drug therapy/microbiology
MH  - Drug Combinations
MH  - Drug Resistance, Fungal
MH  - Drug Synergism
MH  - Eye Infections, Fungal/*drug therapy/microbiology
MH  - Microbial Sensitivity Tests
MH  - Ophthalmic Solutions
MH  - Rabbits
MH  - Tetracycline/*therapeutic use
MH  - Treatment Outcome
EDAT- 2017/08/29 06:00
MHDA- 2017/09/05 06:00
CRDT- 2017/08/29 06:00
AID - 2652821 [pii]
AID - 10.1167/iovs.17-22047 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4292-4298. doi:
      10.1167/iovs.17-22047.