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Allogeneic Descemet's Membrane Transplantation Enhances Corneal Endothelial Monolayer Formation and Restores Functional Integrity Following Descemet's Stripping.

Abstract To characterize the differences in corneal endothelial wound healing in the presence or absence of Descemet's membrane (DM), in vivo.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title investigative ophthalmology & visual science
Publication Year Start




PMID- 28850636
OWN - NLM
STAT- MEDLINE
DA  - 20170829
DCOM- 20170906
LR  - 20170906
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 10
DP  - 2017 Aug 01
TI  - Allogeneic Descemet's Membrane Transplantation Enhances Corneal Endothelial
      Monolayer Formation and Restores Functional Integrity Following Descemet's
      Stripping.
PG  - 4249-4260
LID - 10.1167/iovs.17-22106 [doi]
AB  - Purpose: To characterize the differences in corneal endothelial wound healing in 
      the presence or absence of Descemet's membrane (DM), in vivo. Methods:
      New-Zealand white rabbits were subjected to 7-mm endothelial wound either by
      scraping (n = 8; DM intact), peeling (n = 6; DM removed), or a combinatory
      scrape/peel wound (n = 6). In a second experiment, rabbits underwent peel wound
      with immediate transplantation of pure decellularized human DM (n = 4). In vivo
      endothelial migration was assessed via trypan blue staining. Recovery of corneal 
      clarity and thickness was performed by using slit-lamp biomicroscopy and optical 
      coherence tomography. Cell proliferation, phenotype, and morphology were assessed
      by using immunofluorescence and scanning electron microscopy. Results: In vivo
      wound closure was faster in the presence of DM; 25.4% +/- 1.4%/d versus 5.5% +/- 
      0.6%/d (P < 0.0001). At day 8, complete wound closure was seen in all of the
      scrape samples but none of the peel group, with wound closure preceding clinical 
      recovery by approximately 6 days in the scrape group. Endothelial cells in the
      scraped areas reformed functional monolayers capable of restoring corneal
      thickness and transparency whilst those in the peeled area underwent
      mesenchymal-like transformation resulting in scar formation. Transplanting
      decellularized DM in animals receiving a peel wound resulted in clarity and
      thickness comparable to the scrape group. Endothelial proliferation (Ki67 +ve
      cells) was higher in scraped versus peeled areas: 54.7% +/- 3.5% vs. 8.8% +/-
      0.7%, (P < 0.01). Conclusions: The presence of DM promoted endothelial wound
      healing, proliferation, and maintenance of a normal phenotype. DM transplantation
      recovered the abnormal peel phenotype back to that observed after endothelial
      scraping.
FAU - Bhogal, Maninder
AU  - Bhogal M
AD  - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute,
      Singapore.
AD  - Singapore National Eye Centre, Singapore.
AD  - Institute of Ophthalmology, University College London, London, United Kingdom.
FAU - Lwin, Chan N
AU  - Lwin CN
AD  - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute,
      Singapore.
FAU - Seah, Xin-Yi
AU  - Seah XY
AD  - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute,
      Singapore.
FAU - Peh, Gary
AU  - Peh G
AD  - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute,
      Singapore.
AD  - Ophthalmology Academic Clinical Program, Duke-NUS Graduate Medical School,
      Singapore.
FAU - Mehta, Jodhbir S
AU  - Mehta JS
AD  - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute,
      Singapore.
AD  - Singapore National Eye Centre, Singapore.
AD  - Institute of Ophthalmology, University College London, London, United Kingdom.
AD  - Ophthalmology Academic Clinical Program, Duke-NUS Graduate Medical School,
      Singapore.
AD  - Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Cell Proliferation/physiology
MH  - Corneal Injuries/pathology/physiopathology/*surgery
MH  - Descemet Membrane/*surgery
MH  - Descemet Stripping Endothelial Keratoplasty/*methods
MH  - Disease Models, Animal
MH  - Endothelium, Corneal/cytology/*surgery
MH  - Rabbits
MH  - Recovery of Function/physiology
MH  - Transplantation, Homologous
MH  - Wound Healing/physiology
EDAT- 2017/08/30 06:00
MHDA- 2017/09/07 06:00
CRDT- 2017/08/30 06:00
AID - 2652869 [pii]
AID - 10.1167/iovs.17-22106 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4249-4260. doi:
      10.1167/iovs.17-22106.