PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

DIFFERENT CONCENTRATIONS OF SIJUNZI DECOCTION INHIBIT PROLIFERATION AND INDUCE APOPTOSIS OF HUMAN GASTRIC CANCER SGC-7901 SIDE POPULATION.

Abstract Sijunzi Decoction (SD) is a traditional Chinese medicine which is composed of Ginseng, Atractylodes, Poria and Licorice. It is one of the commonly used Chinese traditional medicines that showed anti-gastric cancer activity in clinical studies. Previous evidence demonstrated SD parties (Ginseng, Atractylodes, Poria, Licorice) can inhibit proliferation and induced apoptosis for gastric cancer cell. In order to further investigate the anticancer effect of SD in gastric cancer, we observed the effects of different concentrations of SD on proliferation and apoptosis of Side Population Cells (SP) of human gastric cancer SGC-7901.
PMID
Related Publications

Adenosine induces cell cycle arrest and apoptosis via cyclinD1/Cdk4 and Bcl-2/Bax pathways in human ovarian cancer cell line OVCAR-3.

Cellular and molecular mechanisms of the Ganoderma applanatum extracts induces apoptosis on SGC-7901 gastric cancer cells.

Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice.

Effect of Sijunzi decoction on the proliferation of side population cells of human gastric cancer cell line.

Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells.

Authors

Mayor MeshTerms
Keywords

Apoptosis

Gastric Cancer

Proliferation

Side Population

Sijunzi Decoction

Journal Title african journal of traditional, complementary, and alternative medicines : ajtcam
Publication Year Start




PMID- 28852730
OWN - NLM
STAT- MEDLINE
DA  - 20170830
DCOM- 20170908
LR  - 20170908
IS  - 2505-0044 (Electronic)
IS  - 2505-0044 (Linking)
VI  - 13
IP  - 4
DP  - 2016
TI  - DIFFERENT CONCENTRATIONS OF SIJUNZI DECOCTION INHIBIT PROLIFERATION AND INDUCE
      APOPTOSIS OF HUMAN GASTRIC CANCER SGC-7901 SIDE POPULATION.
PG  - 145-156
LID - 10.21010/ajtcam.v13i4.19 [doi]
AB  - BACKGROUND: Sijunzi Decoction (SD) is a traditional Chinese medicine which is
      composed of Ginseng, Atractylodes, Poria and Licorice. It is one of the commonly 
      used Chinese traditional medicines that showed anti-gastric cancer activity in
      clinical studies. Previous evidence demonstrated SD parties (Ginseng,
      Atractylodes, Poria, Licorice) can inhibit proliferation and induced apoptosis
      for gastric cancer cell. In order to further investigate the anticancer effect of
      SD in gastric cancer, we observed the effects of different concentrations of SD
      on proliferation and apoptosis of Side Population Cells (SP) of human gastric
      cancer SGC-7901. MATERIALS AND METHODS: SGC-7901 SP and Non- Side Population
      Cells (NSP) were sorted through flow cytometry; to detect the changes of
      proliferation of SP and NSP before and after the intervention of serum containing
      different concentrations of SD using cck-8 method; to detect the changes of cell 
      cycle and apoptosis of SP and NSP before and after the intervention of serum
      containing different concentrations of SD through flow cytometry; to detect the
      effects of serum containing different concentrations of SD on apoptosis-related
      proteins Bax and Bcl-2 of SP and NSP before and after the intervention by
      western-blot. RESULTS: It was found that different concentrations of SD serum
      treatments inhibited cell proliferation in a time-dependent and
      concentration-dependent manner. Compared with the control group (normal saline
      serum treatment), there were increase in G1/G0 phase population of SP and NSP,
      and decrease in G2/M and S phase population (P<0.05). Meanwhile, we found G1/G0
      arrest induced by different concentrations of SD serum which was followed by
      apoptosis in a time-dependent and concentration-dependent manner. The apoptosis
      rate of SD serum treatment group was higher than the control group (P<0.05), the 
      apoptosis rate of 48 h treatment was higher than 24 h treatment (P<0.05), and as 
      the SD serum concentration increases, apoptosis rate is higher and higher
      (P<0.05). The expression of Bax protein of SP and NSP was higher than the control
      group in a time-dependent and concentration dependent manner. The expression of
      Bcl-2 protein of SP and NSP was lower than the control group in a time-dependent 
      and concentration- dependent manner. CONCLUSION: With the increase of SD serum
      concentrations, SD can gradually inhibits the proliferation of SP of SGC-7901
      cell lines through G1/G0 phase arrest and followed by apoptosis which involves
      the up-regulation of Bax and the down-regulation of Bcl-2. List of Abbreviations:
      (SD) Sijunzi Decoction, (SP) side population, (NSP) non-side population,
      (Control) normal saline serum group, (L) low concentration SD serum group, (N)
      normal concentration SD serum group, (H) high concentration SD serum group,
      (ABCG-2) Adenosine triphosphate Binding Cassette super family G member-2 of
      transport protein, (Bcl-2) B-cell lymphoma 2, (BAX) Bcl-2 Associated X Protein,
      (FBS) Fetal bovine serum, (PBS) Phosphate buffer solution, (CCK-8) Cell Counting 
      Kit-8 reagent, (AV) Annexin V-FITC, (PI) Propidium iodide, (EDTA) Ethylene
      Diamine Tetraacetic Acid, (PMSF) Phenylmethanesulfonyl fluoride, (RIPA) Radio
      Immunoprecipitation Assay, (PVDF) Poly (vinylidene fluoride), (TBST)
      Tris-buffered saline containing Tween-20.
FAU - Qian, Jun
AU  - Qian J
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Li, Jing
AU  - Li J
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Jia, Jianguang
AU  - Jia J
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Jin, Xin
AU  - Jin X
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Yu, Dajun
AU  - Yu D
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Guo, Chenxu
AU  - Guo C
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Xie, Bo
AU  - Xie B
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
FAU - Qian, Liyu
AU  - Qian L
AD  - Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical
      College, Bengbu 233000, PR China.
LA  - eng
PT  - Journal Article
DEP - 20160703
PL  - Nigeria
TA  - Afr J Tradit Complement Altern Med
JT  - African journal of traditional, complementary, and alternative medicines : AJTCAM
JID - 101232990
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Down-Regulation
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Humans
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Stomach Neoplasms/drug therapy/genetics/metabolism/*physiopathology
MH  - bcl-2-Associated X Protein/genetics/metabolism
PMC - PMC5566137
OTO - NOTNLM
OT  - Apoptosis
OT  - Gastric Cancer
OT  - Proliferation
OT  - Side Population
OT  - Sijunzi Decoction
EDAT- 2017/08/31 06:00
MHDA- 2017/09/09 06:00
CRDT- 2017/08/31 06:00
AID - 10.21010/ajtcam.v13i4.19 [doi]
AID - AJTCAM-13-145 [pii]
PST - epublish
SO  - Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):145-156. doi:
      10.21010/ajtcam.v13i4.19. eCollection 2016.