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PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION.

Abstract Damage of the blood brain barrier (BBB) during the process of cerebral ischemic injury is a key factor which influences the therapeutic efficacy to the cerebral ischemic injury. The present study was designed to verify the mechanisms underlying the protective effects of the ethyl acetate (EtOAc) extraction from Gastrodia elata Blume (GEB) on the BBB by developing a model of cerebral ischemia-reperfusion in rats.
PMID
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Authors

Mayor MeshTerms
Keywords

AQP-4

Gastrodia elata Blume

TJ

blood-brain barrier

Journal Title african journal of traditional, complementary, and alternative medicines : ajtcam
Publication Year Start




PMID- 28852737
OWN - NLM
STAT- MEDLINE
DA  - 20170830
DCOM- 20170908
LR  - 20170908
IS  - 2505-0044 (Electronic)
IS  - 2505-0044 (Linking)
VI  - 13
IP  - 4
DP  - 2016
TI  - PROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACTION FROM GASTRODIA ELATA BLUME ON
      BLOOD-BRAIN BARRIER IN FOCAL CEREBRAL ISCHEMIA REPERFUSION.
PG  - 199-209
LID - 10.21010/ajtcam.v13i4.26 [doi]
AB  - BACKGROUND: Damage of the blood brain barrier (BBB) during the process of
      cerebral ischemic injury is a key factor which influences the therapeutic
      efficacy to the cerebral ischemic injury. The present study was designed to
      verify the mechanisms underlying the protective effects of the ethyl acetate
      (EtOAc) extraction from Gastrodia elata Blume (GEB) on the BBB by developing a
      model of cerebral ischemia-reperfusion in rats. MATERIAL AND METHODS: MCAO/R
      model in rats was developed through a thread embolism method. The neurological
      scales, the moisture and the evans blue (EB) contents of brains were detected.
      Meanwhile, the release of nitric oxide (NO) and activities of NO synthase (NOS)
      in brain tissues were measured. Western blotting analyses were also performed to 
      assess the protein expressions of AQP-4, Occludin and Claudin-5 in brain tissue. 
      RESULTS: After rats were pretreated with different concentrations of EtOAc
      extractions from GEB, the neurologic scores, the EB contents in the brain tissues
      and the moisture of the brains were significantly decreased. Meanwhile, the
      release of NO, the activities of nNOS and iNOS were notably inhibited.
      Furthemore, the protein expression of AQP-4 was markedly decreased, but the
      protein expressions of -5 and Occludin were significantly increased. CONCLUSION: 
      the EtOAc extracts of GEB may decrease the permeability of BBB when focal
      cerebral ischemia occurs. The inhibition of the NOS pathways, the attenuation of 
      the protein expression of AQP-4 and the enhancement of the expressions of the
      tight junction proteins may contribute to the protective effects of the EtOAc
      extracts from GEB on BBB.
FAU - He, Fangyan
AU  - He F
AD  - The Department of Pharmacology, Yunnan University of Traditional Chinese
      Medicine, Kunming 650500, China.
FAU - Duan, Xiaohua
AU  - Duan X
AD  - The Key Modern Research Laboratory for Ethno-pharmacognosy of Yunnan Higher
      School, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
FAU - Dai, Rong
AU  - Dai R
AD  - The Department of Pharmacology, Yunnan University of Traditional Chinese
      Medicine, Kunming 650500, China.
FAU - Wang, Wei
AU  - Wang W
AD  - The Department of Pharmacology, Yunnan University of Traditional Chinese
      Medicine, Kunming 650500, China.
FAU - Yang, Cui
AU  - Yang C
AD  - Ethnic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in
      Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of
      Education, Yunnan Minzu University, Kunming 650500, China.
FAU - Lin, Qing
AU  - Lin Q
AD  - The Department of Pharmacology, Yunnan University of Traditional Chinese
      Medicine, Kunming 650500, China.
LA  - eng
PT  - Journal Article
DEP - 20160703
PL  - Nigeria
TA  - Afr J Tradit Complement Altern Med
JT  - African journal of traditional, complementary, and alternative medicines : AJTCAM
JID - 101232990
RN  - 0 (Acetates)
RN  - 0 (Aqp4 protein, rat)
RN  - 0 (Aquaporin 4)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Occludin)
RN  - 0 (Protective Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 76845O8NMZ (ethyl acetate)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Acetates/chemistry
MH  - Animals
MH  - Aquaporin 4/genetics/metabolism
MH  - Blood-Brain Barrier/*drug effects/metabolism
MH  - Brain Ischemia/surgery
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Gastrodia/*chemistry
MH  - Humans
MH  - Male
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/genetics/metabolism
MH  - Occludin/genetics/metabolism
MH  - Protective Agents/*administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/genetics/metabolism/*prevention & control
PMC - PMC5566145
OTO - NOTNLM
OT  - AQP-4
OT  - Gastrodia elata Blume
OT  - TJ
OT  - blood-brain barrier
EDAT- 2017/08/31 06:00
MHDA- 2017/09/09 06:00
CRDT- 2017/08/31 06:00
AID - 10.21010/ajtcam.v13i4.26 [doi]
AID - AJTCAM-13-199 [pii]
PST - epublish
SO  - Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):199-209. doi:
      10.21010/ajtcam.v13i4.26. eCollection 2016.