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N-glycosylation of the transient receptor potential melastatin 8 channel is altered in pancreatic cancer cells.

Abstract Transient receptor potential melastatin 8 (TRPM8), a membrane ion channel, is activated by thermal and chemical stimuli. In pancreatic ductal adenocarcinoma, TRPM8 is required for cell migration, proliferation, and senescence and is associated with tumor size and pancreatic ductal adenocarcinoma stages. Although the underlying mechanisms of these processes have yet to be described, this cation-permeable channel has been proposed as an oncological target. In this study, the glycosylation status of the TRPM8 channel was shown to affect cell proliferation, cell migration, and calcium uptake. TRPM8 expressed in the membrane of the Panc-1 pancreatic tumoral cell line is non-glycosylated, whereas human embryonic kidney cells transfected with human TRPM8 overexpress a glycosylated protein. Moreover, our data suggest that Ca(2+) uptake is modulated by the glycosylation status of the protein, thus affecting cell proliferation.
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Authors

Mayor MeshTerms
Keywords

Pancreatic cancer

cell migration

glycosylation

membrane protein

patch clamp

transient receptor potential melastatin 8

Journal Title tumour biology : the journal of the international society for oncodevelopmental biology and medicine
Publication Year Start




PMID- 28857015
OWN - NLM
STAT- In-Process
DA  - 20170831
LR  - 20170831
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 39
IP  - 8
DP  - 2017 Aug
TI  - N-glycosylation of the transient receptor potential melastatin 8 channel is
      altered in pancreatic cancer cells.
PG  - 1010428317720940
LID - 10.1177/1010428317720940 [doi]
AB  - Transient receptor potential melastatin 8 (TRPM8), a membrane ion channel, is
      activated by thermal and chemical stimuli. In pancreatic ductal adenocarcinoma,
      TRPM8 is required for cell migration, proliferation, and senescence and is
      associated with tumor size and pancreatic ductal adenocarcinoma stages. Although 
      the underlying mechanisms of these processes have yet to be described, this
      cation-permeable channel has been proposed as an oncological target. In this
      study, the glycosylation status of the TRPM8 channel was shown to affect cell
      proliferation, cell migration, and calcium uptake. TRPM8 expressed in the
      membrane of the Panc-1 pancreatic tumoral cell line is non-glycosylated, whereas 
      human embryonic kidney cells transfected with human TRPM8 overexpress a
      glycosylated protein. Moreover, our data suggest that Ca2+ uptake is modulated by
      the glycosylation status of the protein, thus affecting cell proliferation.
FAU - Ulareanu, Roxana
AU  - Ulareanu R
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Chiritoiu, Gabriela
AU  - Chiritoiu G
AD  - 2 Department of Molecular Cell Biology, Institute of Biochemistry, Romanian
      Academy, Bucharest, Romania.
FAU - Cojocaru, Florentina
AU  - Cojocaru F
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Deftu, Alexandru
AU  - Deftu A
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Ristoiu, Violeta
AU  - Ristoiu V
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Stanica, Luciana
AU  - Stanica L
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Mihailescu, Dan F
AU  - Mihailescu DF
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
FAU - Cucu, Dana
AU  - Cucu D
AD  - 1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology,
      University of Bucharest, Bucharest, Romania.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental
      Biology and Medicine
JID - 8409922
OTO - NOTNLM
OT  - Pancreatic cancer
OT  - cell migration
OT  - glycosylation
OT  - membrane protein
OT  - patch clamp
OT  - transient receptor potential melastatin 8
EDAT- 2017/09/01 06:00
MHDA- 2017/09/01 06:00
CRDT- 2017/09/01 06:00
AID - 10.1177/1010428317720940 [doi]
PST - ppublish
SO  - Tumour Biol. 2017 Aug;39(8):1010428317720940. doi: 10.1177/1010428317720940.