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Influence of partial hepatectomie on ammoniumdetoxications function of liver at chronic tetrachlorcarbon hepatitis.

Abstract The purpose. To study the effect of partial hepatectomy (PH) on the main ways of ammonia detoxication in the liver (synthesis of urea and glutamine) in chronic tetrachlorcarbon (CCl4) hepatitis. Methods. The experiments were performed on 165 white outbred rats (females) weighing 180-220 g Chronic CCl4-hepatitis was reproduced by subcutaneous injection of 50% CCl4 solution in olive oil (0.1 ml/100g of body weight,65 days, through the day with two two-week breaks between 6-7 and 13-14 injections). PH conducted electrocautery, removing part of the left lobe of the liver (15-20% by weight of the body) to 65th (and last) day of the introduction of the CCl4. Animals were studied after 65 days of development of CCl4-hepatitis on day 3, 7 and 14 days after laparotomy («falsely operated» animals) and partial hepatectomy. In subcellular fractions of the liver investigated the activity of phosphatdependent glutaminase (FDG), glutamatdehydrogenaze (GDG), glutaminsintetaze (GS), arginase. In the liver tissue investigated the content of ammonia, glutamine, glutamate and urea. Results. Found that on 65-th day of the development of CCl4 in the liver decreases the concentration of ammonia, glutamine, glutamate, urea, and activity of GS, GDG and arginase. Activity FDG was not changed. The use of PH on the background of chronic CCl4-hepatitis has a short-term (3 days) a stimulating effect on the activity FDG, GS, postponed (14 days) the inhibitory effect on the activity of GDG. This was accompanied by an increase in the concentration in the liver of ammonia within 14 days of the postoperative period on the background of maintaining reduced concentrations of glutamine and glutamate it. The stimulatory effect of CHA on the activity of arginase is saved to the 14th day of the postoperative period, however, the concentration of urea in the liver remained below normal. Conclusions. The obtained results show that CGA on the background of chronic hepatitis increases the pathological impact of CCl4 on amniocentesis liver function.
PMID
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Authors

Mayor MeshTerms

Carbon Tetrachloride

Hepatectomy

Keywords
Journal Title patologicheskaia fiziologiia i eksperimental'naia terapiia
Publication Year Start




PMID- 29215843
OWN - NLM
STAT- MEDLINE
DCOM- 20171221
LR  - 20171221
IS  - 0031-2991 (Print)
IS  - 0031-2991 (Linking)
VI  - 61
IP  - 2
DP  - 2017 Apr-Jun
TI  - [Influence of partial hepatectomie on ammoniumdetoxications function of liver at 
      chronic tetrachlorcarbon hepatitis].
PG  - 61-6
AB  - The purpose. To study the effect of partial hepatectomy (PH) on the main ways of 
      ammonia detoxication in the liver (synthesis of urea and glutamine) in chronic
      tetrachlorcarbon (CCl4) hepatitis. Methods. The experiments were performed on 165
      white outbred rats (females) weighing 180-220 g Chronic CCl4-hepatitis was
      reproduced by subcutaneous injection of 50% CCl4 solution in olive oil (0.1
      ml/100g of body weight,65 days, through the day with two two-week breaks between 
      6-7 and 13-14 injections). PH conducted electrocautery, removing part of the left
      lobe of the liver (15-20% by weight of the body) to 65th (and last) day of the
      introduction of the CCl4. Animals were studied after 65 days of development of
      CCl4-hepatitis on day 3, 7 and 14 days after laparotomy (<<falsely operated>>
      animals) and partial hepatectomy. In subcellular fractions of the liver
      investigated the activity of phosphatdependent glutaminase (FDG),
      glutamatdehydrogenaze (GDG), glutaminsintetaze (GS), arginase. In the liver
      tissue investigated the content of ammonia, glutamine, glutamate and urea.
      Results. Found that on 65-th day of the development of CCl4 in the liver
      decreases the concentration of ammonia, glutamine, glutamate, urea, and activity 
      of GS, GDG and arginase. Activity FDG was not changed. The use of PH on the
      background of chronic CCl4-hepatitis has a short-term (3 days) a stimulating
      effect on the activity FDG, GS, postponed (14 days) the inhibitory effect on the 
      activity of GDG. This was accompanied by an increase in the concentration in the 
      liver of ammonia within 14 days of the postoperative period on the background of 
      maintaining reduced concentrations of glutamine and glutamate it. The stimulatory
      effect of CHA on the activity of arginase is saved to the 14th day of the
      postoperative period, however, the concentration of urea in the liver remained
      below normal. Conclusions. The obtained results show that CGA on the background
      of chronic hepatitis increases the pathological impact of CCl4 on amniocentesis
      liver function.
FAU - Savilov, P N
AU  - Savilov PN
LA  - rus
PT  - Journal Article
PL  - Russia (Federation)
TA  - Patol Fiziol Eksp Ter
JT  - Patologicheskaia fiziologiia i eksperimental'naia terapiia
JID - 0376421
RN  - 0RH81L854J (Glutamine)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 7664-41-7 (Ammonia)
RN  - 8W8T17847W (Urea)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Animals
MH  - *Carbon Tetrachloride
MH  - Carbon Tetrachloride Poisoning/*metabolism/pathology
MH  - Chemical and Drug Induced Liver Injury/*metabolism/pathology
MH  - Chronic Disease
MH  - Female
MH  - Glutamic Acid/metabolism
MH  - Glutamine/metabolism
MH  - *Hepatectomy
MH  - Liver/*metabolism/pathology
MH  - Rats
MH  - Urea/metabolism
EDAT- 2017/12/08 06:00
MHDA- 2017/12/22 06:00
CRDT- 2017/12/08 06:00
PHST- 2017/12/08 06:00 [entrez]
PHST- 2017/12/08 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
PST - ppublish
SO  - Patol Fiziol Eksp Ter. 2017 Apr-Jun;61(2):61-6.