PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Detection and sequencing of rotavirus among sudanese children.

Abstract Diarrheal diseases are a big public health problem worldwide, particularly among developing countries. The current study was conducted to detect and characterize group A rotavirus among admitted children with gastroenteritis to the pediatric hospitals, Sudan.
PMID
Related Publications
Authors

Mayor MeshTerms
Keywords

Rotavirus

Sudan

VP4

VP7

children

gastroenteritis

Journal Title the pan african medical journal
Publication Year Start




PMID- 29255557
OWN - NLM
STAT- MEDLINE
DCOM- 20171222
LR  - 20171222
IS  - 1937-8688 (Electronic)
VI  - 28
DP  - 2017
TI  - Detection and sequencing of rotavirus among sudanese children.
PG  - 87
LID - 10.11604/pamj.2017.28.87.11008 [doi]
AB  - Introduction: Diarrheal diseases are a big public health problem worldwide,
      particularly among developing countries. The current study was conducted to
      detect and characterize group A rotavirus among admitted children with
      gastroenteritis to the pediatric hospitals, Sudan. Methods: A total of 755 stool 
      samples were collected from Sudanese children with less than 5 years of age
      presenting with acute gastroenteritis during the period from April to September
      2010. Enzyme-linked immunosorbent assay (ELISA) was used to Detection of
      Rotavirus antigens. Ribonucleic acid (RNAs) were extracted from
      rotavirus-positive stool samples using (QIAamp((R)) Viral RNA Mini Kit).
      (Omniscript((R)) Reverse Transcription kit) was used to convert RNA to
      complementary Deoxyribonucleic acid (cDNA). The cDNAs were used as template for
      detection of VP4-P (P for Protease-sensitive) and VP7-G (G for Glycoprotein)
      genotyping of Rotavirus using nested PCR and sequencing. Results: Out of the 755 
      stool samples from children with acute gastroenteritis, 121 were positive for
      rotavirus A. Among 24 samples that were sequenced; the VP7 predominant G type was
      G1 (83.3%), followed by G9 (16.7%). Out of these samples, only one VP4 P[8]
      genotype was detected. Conclusion: As a conclusion the VP7 predominant G type was
      G1, followed by G9 whereas only one VP4 genotype was detected and showed
      similarity to P[8] GenBank strain. It appears that the recently approved
      rotavirus vaccines in Sudan are well matched to the rotavirus genotypes
      identified in this study, though more studies are needed.
FAU - Magzoub, Magzoub Abbas
AU  - Magzoub MA
AD  - National Public Health Laboratory, Ministry of Health, Khartoum, Sudan.
AD  - Faculty of Medical Laboratory Sciences, Khartoum University, Khartoum, Sudan.
AD  - College of Applied Medical Science, Qassim University, Buraydah, Saudi Arabia.
FAU - Bilal, Naser Eldin
AU  - Bilal NE
AD  - Faculty of Medical Laboratory Sciences, Khartoum University, Khartoum, Sudan.
FAU - Bilal, Jalal Ali
AU  - Bilal JA
AD  - College of Medicine, Qassim University, Qassim, Saudi Arabia.
FAU - Alzohairy, Mohammad Abdulrahman
AU  - Alzohairy MA
AD  - College of Applied Medical Science, Qassim University, Buraydah, Saudi Arabia.
FAU - Elamin, Bahaeldin Khalid
AU  - Elamin BK
AD  - Faculty of Medical Laboratory Sciences, Khartoum University, Khartoum, Sudan.
AD  - College of Medicine, Bisha University, Bisha, Saudi Arabia.
FAU - Gasim, Gasim Ibrahim
AU  - Gasim GI
AD  - College of Medicine, Qassim University, Qassim, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20170927
PL  - Uganda
TA  - Pan Afr Med J
JT  - The Pan African medical journal
JID - 101517926
SB  - IM
MH  - Acute Disease
MH  - Child, Preschool
MH  - Cross-Sectional Studies
MH  - Diarrhea/*epidemiology/virology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Gastroenteritis/diagnosis/*epidemiology/virology
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Rotavirus/*genetics/isolation & purification
MH  - Rotavirus Infections/diagnosis/*epidemiology/virology
MH  - Sudan/epidemiology
PMC - PMC5724952
OTO - NOTNLM
OT  - Rotavirus
OT  - Sudan
OT  - VP4
OT  - VP7
OT  - children
OT  - gastroenteritis
EDAT- 2017/12/20 06:00
MHDA- 2017/12/23 06:00
CRDT- 2017/12/20 06:00
PHST- 2016/10/22 00:00 [received]
PHST- 2017/08/16 00:00 [accepted]
PHST- 2017/12/20 06:00 [entrez]
PHST- 2017/12/20 06:00 [pubmed]
PHST- 2017/12/23 06:00 [medline]
AID - 10.11604/pamj.2017.28.87.11008 [doi]
AID - PAMJ-28-87 [pii]
PST - epublish
SO  - Pan Afr Med J. 2017 Sep 27;28:87. doi: 10.11604/pamj.2017.28.87.11008.
      eCollection 2017.