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PMID- 29258473
OWN - NLM
STAT- MEDLINE
DCOM- 20180105
LR  - 20180105
IS  - 1471-2415 (Electronic)
IS  - 1471-2415 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Dec 19
TI  - Proteomics analysis and proteogenomic characterization of different
      physiopathological human lenses.
PG  - 253
LID - 10.1186/s12886-017-0642-9 [doi]
AB  - BACKGROUND: The aim of the present study was to identify the proteomic
      differences among human lenses in different physiopathological states and to
      screen for susceptibility genes/proteins via proteogenomic characterization.
      METHODS: The total proteomes identified across the regenerative lens with
      secondary cataract (RLSC), congenital cataract (CC) and age-related cataract
      (ARC) groups were compared to those of normal lenses using isobaric tagging for
      relative and absolute protein quantification (iTRAQ). The up-regulated proteins
      between the groups were subjected to biological analysis. Whole exome sequencing 
      (WES) was performed to detect genetic variations. RESULTS: The most complete
      human lens proteome to date, which consisted of 1251 proteins, including 55.2%
      previously unreported proteins, was identified across the experimental groups.
      Bioinformatics functional annotation revealed the common involvement of cellular 
      metabolic processes, immune responses and protein folding disturbances among the 
      groups. RLSC-over-expressed proteins were characteristically enriched in the
      intracellular immunological signal transduction pathways. The CC groups featured 
      biological processes relating to gene expression and vascular endothelial growth 
      factor (VEGF) signaling transduction, whereas the molecular functions
      corresponding to external stress were specific to the ARC groups. Combined with
      WES, the proteogenomic characterization narrowed the list to 16 candidate causal 
      molecules. CONCLUSIONS: These findings revealed common final pathways with
      diverse upstream regulation of cataractogenesis in different physiopathological
      states. This proteogenomic characterization shows translational potential for
      detecting susceptibility genes/proteins in precision medicine.
FAU - Wu, Xiaohang
AU  - Wu X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Liu, Zhenzhen
AU  - Liu Z
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Zhang, Xiayin
AU  - Zhang X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Wang, Dongni
AU  - Wang D
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Long, Erping
AU  - Long E
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Wang, Jinghui
AU  - Wang J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Li, Wangting
AU  - Li W
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Lai, Weiyi
AU  - Lai W
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Cao, Qianzhong
AU  - Cao Q
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Hu, Kunhua
AU  - Hu K
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University,
      Guangzhou, Guangdong, 510080, China.
FAU - Chen, Weirong
AU  - Chen W
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
FAU - Lin, Haotian
AU  - Lin H
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
      [email protected]
FAU - Liu, Yizhi
AU  - Liu Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen
      University, 54# Xianlie Road, Guangzhou, Guangdong, 510060, China.
      [email protected]
LA  - eng
GR  - 2015CB964600/The Ministry of Science and Technology of China Grants/United States
GR  - 91546101/The Key Research Plan for the National Natural Science Foundation of
      China in Cultivation Project/United States
GR  - 81300750/The National Natural Science Foundation of China/United States
GR  - NA/the Guangdong Province Universities and Colleges Youth Pearl River Scholar
      Funded Scheme/United States
GR  - YQ2015006/The Outstanding Young Teacher Cultivation Projects in Guangdong
      Province/United States
GR  - 2014A030306030/The Guangdong Provincial Natural Science Foundation for
      Distinguished Young Scholars of China/United States
GR  - 2014TQ01R573/The Youth Science and Technology Innovation Talents Funds in a
      Special Support Plan for High Level Talents in Guangdong Province/United States
GR  - 2015ykzd11/The Young Teacher Top-Support project of Sun Yat-sen University/United
      States
GR  - 2017QN04/the Fundamental Research Funds of the State Key Laboratory of
      Ophthalmology/United States
PT  - Journal Article
DEP - 20171219
PL  - England
TA  - BMC Ophthalmol
JT  - BMC ophthalmology
JID - 100967802
RN  - 0 (Eye Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Adult
MH  - Cataract/*metabolism
MH  - Child, Preschool
MH  - Chromatography, Liquid
MH  - Eye Proteins/*metabolism
MH  - Female
MH  - Humans
MH  - Lens, Crystalline/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Proteogenomics
MH  - Proteome/*analysis/genetics
MH  - Proteomics
MH  - Tandem Mass Spectrometry
MH  - Young Adult
PMC - PMC5735820
OTO - NOTNLM
OT  - Human lens
OT  - Proteogenomic analysis
OT  - Proteomics
OT  - Regenerative lenses with secondary cataract (RLSC)
EDAT- 2017/12/21 06:00
MHDA- 2018/01/06 06:00
CRDT- 2017/12/21 06:00
PHST- 2017/04/04 00:00 [received]
PHST- 2017/12/04 00:00 [accepted]
PHST- 2017/12/21 06:00 [entrez]
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2018/01/06 06:00 [medline]
AID - 10.1186/s12886-017-0642-9 [doi]
AID - 10.1186/s12886-017-0642-9 [pii]
PST - epublish
SO  - BMC Ophthalmol. 2017 Dec 19;17(1):253. doi: 10.1186/s12886-017-0642-9.