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Distinct virulence of Rift Valley fever phlebovirus strains from different genetic lineages in a mouse model.

Abstract Rift Valley fever phlebovirus (RVFV) causes high rates of abortions and fetal malformations in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral transmission occurs via mosquito vectors in endemic areas, which necessitates regular vaccination of susceptible livestock animals to prevent the RVF outbreaks. Although ZH501 strain has been used as a challenge strain for past vaccine efficacy studies, further characterization of other RVFV strains is important to optimize ruminant and nonhuman primate RVFV challenge models. This study aimed to characterize the virulence of wild-type RVFV strains belonging to different genetic lineages in outbred CD1 mice. Mice were intraperitoneally infected with 1x103 PFU of wild-type ZH501, Kenya 9800523, Kenya 90058, Saudi Arabia 200010911, OS1, OS7, SA75, Entebbe, or SA51 strains. Among them, mice infected with SA51, Entebbe, or OS7 strain showed rapid dissemination of virus in livers and peracute necrotic hepatitis at 2-3 dpi. Recombinant SA51 (rSA51) and Zinga (rZinga) strains were recovered by reverse genetics, and their virulence was also tested in CD1 mice. The rSA51 strain reproduced peracute RVF disease in mice, whereas the rZinga strain showed a similar virulence with that of rZH501 strain. This study showed that RVFV strains in different genetic lineages display distinct virulence in outbred mice. Importantly, since wild-type RVFV strains contain defective-interfering RNA or various genetic subpopulations during passage from original viral isolations, recombinant RVFV strains generated by reverse genetics will be better suitable for reproducible challenge studies for vaccine development as well as pathological studies.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos one
Publication Year Start




PMID- 29267298
OWN - NLM
STAT- In-Process
LR  - 20180111
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 12
DP  - 2017
TI  - Distinct virulence of Rift Valley fever phlebovirus strains from different
      genetic lineages in a mouse model.
PG  - e0189250
LID - 10.1371/journal.pone.0189250 [doi]
AB  - Rift Valley fever phlebovirus (RVFV) causes high rates of abortions and fetal
      malformations in ruminants, and hemorrhagic fever, encephalitis, or blindness in 
      humans. Viral transmission occurs via mosquito vectors in endemic areas, which
      necessitates regular vaccination of susceptible livestock animals to prevent the 
      RVF outbreaks. Although ZH501 strain has been used as a challenge strain for past
      vaccine efficacy studies, further characterization of other RVFV strains is
      important to optimize ruminant and nonhuman primate RVFV challenge models. This
      study aimed to characterize the virulence of wild-type RVFV strains belonging to 
      different genetic lineages in outbred CD1 mice. Mice were intraperitoneally
      infected with 1x103 PFU of wild-type ZH501, Kenya 9800523, Kenya 90058, Saudi
      Arabia 200010911, OS1, OS7, SA75, Entebbe, or SA51 strains. Among them, mice
      infected with SA51, Entebbe, or OS7 strain showed rapid dissemination of virus in
      livers and peracute necrotic hepatitis at 2-3 dpi. Recombinant SA51 (rSA51) and
      Zinga (rZinga) strains were recovered by reverse genetics, and their virulence
      was also tested in CD1 mice. The rSA51 strain reproduced peracute RVF disease in 
      mice, whereas the rZinga strain showed a similar virulence with that of rZH501
      strain. This study showed that RVFV strains in different genetic lineages display
      distinct virulence in outbred mice. Importantly, since wild-type RVFV strains
      contain defective-interfering RNA or various genetic subpopulations during
      passage from original viral isolations, recombinant RVFV strains generated by
      reverse genetics will be better suitable for reproducible challenge studies for
      vaccine development as well as pathological studies.
FAU - Ikegami, Tetsuro
AU  - Ikegami T
AUID- ORCID: 0000-0001-8318-2783
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Sealy Center for Vaccine Development, The University of Texas Medical Branch,
      Galveston, Texas, United States of America.
AD  - Center for Biodefense and Emerging Infectious Diseases, The University of Texas
      Medical Branch, Galveston, Texas, United States of America.
FAU - Balogh, Aaron
AU  - Balogh A
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,
      Kansas State University, Manhattan, Kansas, United States of America.
FAU - Nishiyama, Shoko
AU  - Nishiyama S
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Lokugamage, Nandadeva
AU  - Lokugamage N
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Saito, Tais B
AU  - Saito TB
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Morrill, John C
AU  - Morrill JC
AD  - Department of Microbiology and Immunology, The University of Texas Medical
      Branch, Galveston, Texas, United States of America.
FAU - Shivanna, Vinay
AU  - Shivanna V
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,
      Kansas State University, Manhattan, Kansas, United States of America.
FAU - Indran, Sabarish V
AU  - Indran SV
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,
      Kansas State University, Manhattan, Kansas, United States of America.
FAU - Zhang, Lihong
AU  - Zhang L
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Galveston National Laboratory, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Smith, Jennifer K
AU  - Smith JK
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Galveston National Laboratory, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Perez, David
AU  - Perez D
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Galveston National Laboratory, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Juelich, Terry L
AU  - Juelich TL
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Galveston National Laboratory, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Morozov, Igor
AU  - Morozov I
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,
      Kansas State University, Manhattan, Kansas, United States of America.
FAU - Wilson, William C
AU  - Wilson WC
AD  - United States Department of Agriculture, Agricultural Research Service, Arthropod
      Borne Animal Diseases Research Unit, Manhattan, Kansas, United States of America.
FAU - Freiberg, Alexander N
AU  - Freiberg AN
AD  - Department of Pathology, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
AD  - Sealy Center for Vaccine Development, The University of Texas Medical Branch,
      Galveston, Texas, United States of America.
AD  - Center for Biodefense and Emerging Infectious Diseases, The University of Texas
      Medical Branch, Galveston, Texas, United States of America.
AD  - Galveston National Laboratory, The University of Texas Medical Branch, Galveston,
      Texas, United States of America.
FAU - Richt, Juergen A
AU  - Richt JA
AD  - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,
      Kansas State University, Manhattan, Kansas, United States of America.
LA  - eng
GR  - R01 AI08764301-A1 /NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, N.I.H., Extramural
DEP - 20171221
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
PMC - PMC5739399
EDAT- 2017/12/22 06:00
MHDA- 2017/12/22 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/08/14 00:00 [received]
PHST- 2017/11/23 00:00 [accepted]
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2017/12/22 06:00 [medline]
AID - 10.1371/journal.pone.0189250 [doi]
AID - PONE-D-17-40932 [pii]
PST - epublish
SO  - PLoS One. 2017 Dec 21;12(12):e0189250. doi: 10.1371/journal.pone.0189250.
      eCollection 2017.