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Association of Marek's Disease induced immunosuppression with activation of a novel regulatory T cells in chickens.

Abstract Marek's Disease Virus (MDV) is an alphaherpesvirus that infects chickens, transforms CD4+ T cells and causes deadly lymphomas. In addition, MDV induces immunosuppression early during infection by inducing cell death of the infected lymphocytes, and potentially due to activation of regulatory T (Treg)-cells. Furthermore, immunosuppression also occurs during the transformation phase of the disease; however, it is still unknown how the disease can suppress immune response prior or after lymphoma formation. Here, we demonstrated that chicken TGF-beta+ Treg cells are found in different lymphoid tissues, with the highest levels found in the gut-associated lymphoid tissue (cecal tonsil: CT), fostering an immune-privileged microenvironment exerted by TGF-beta. Surprisingly, significantly higher frequencies of TGF-beta+ Treg cells are found in the spleens of MDV-susceptible chicken lines compared to the resistant line, suggesting an association between TGF-beta+ Treg cells and host susceptibility to lymphoma formation. Experimental infection with a virulent MDV elevated the levels of TGF-beta+ Treg cells in the lungs as early as 4 days post infection, and during the transformation phase of the disease in the spleens. In contrast to TGF-beta+ Treg cells, the levels of CD4+CD25+ T cells remained unchanged during the infection and transformation phase of the disease. Furthermore, our results demonstrate that the induction of TGF-beta+ Treg cells is associated with pathogenesis of the disease, as the vaccine strain of MDV did not induce TGF-beta+ Treg cells. Similar to human haematopoietic malignant cells, MDV-induced lymphoma cells expressed high levels of TGF-beta but very low levels of TGF-beta receptor I and II genes. The results confirm that COX-2/ PGE2 pathway is involved in immunosuppression induced by MDV-lymphoma cells. Taken together, our results revealed a novel TGF-beta+ Treg subset in chickens that is activated during MDV infection and tumour formation.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title plos pathogens
Publication Year Start




PMID- 29267390
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20180111
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Linking)
VI  - 13
IP  - 12
DP  - 2017 Dec
TI  - Association of Marek's Disease induced immunosuppression with activation of a
      novel regulatory T cells in chickens.
PG  - e1006745
LID - 10.1371/journal.ppat.1006745 [doi]
AB  - Marek's Disease Virus (MDV) is an alphaherpesvirus that infects chickens,
      transforms CD4+ T cells and causes deadly lymphomas. In addition, MDV induces
      immunosuppression early during infection by inducing cell death of the infected
      lymphocytes, and potentially due to activation of regulatory T (Treg)-cells.
      Furthermore, immunosuppression also occurs during the transformation phase of the
      disease; however, it is still unknown how the disease can suppress immune
      response prior or after lymphoma formation. Here, we demonstrated that chicken
      TGF-beta+ Treg cells are found in different lymphoid tissues, with the highest
      levels found in the gut-associated lymphoid tissue (cecal tonsil: CT), fostering 
      an immune-privileged microenvironment exerted by TGF-beta. Surprisingly,
      significantly higher frequencies of TGF-beta+ Treg cells are found in the spleens
      of MDV-susceptible chicken lines compared to the resistant line, suggesting an
      association between TGF-beta+ Treg cells and host susceptibility to lymphoma
      formation. Experimental infection with a virulent MDV elevated the levels of
      TGF-beta+ Treg cells in the lungs as early as 4 days post infection, and during
      the transformation phase of the disease in the spleens. In contrast to TGF-beta+ 
      Treg cells, the levels of CD4+CD25+ T cells remained unchanged during the
      infection and transformation phase of the disease. Furthermore, our results
      demonstrate that the induction of TGF-beta+ Treg cells is associated with
      pathogenesis of the disease, as the vaccine strain of MDV did not induce
      TGF-beta+ Treg cells. Similar to human haematopoietic malignant cells,
      MDV-induced lymphoma cells expressed high levels of TGF-beta but very low levels 
      of TGF-beta receptor I and II genes. The results confirm that COX-2/ PGE2 pathway
      is involved in immunosuppression induced by MDV-lymphoma cells. Taken together,
      our results revealed a novel TGF-beta+ Treg subset in chickens that is activated 
      during MDV infection and tumour formation.
FAU - Gurung, Angila
AU  - Gurung A
AUID- ORCID: 0000-0001-7817-2505
AD  - The Pirbright Institute, Ash Road, Woking, United Kingdom.
AD  - College of Health and Life Sciences, Department of Life Sciences, Brunel
      University, London, United Kingdom.
FAU - Kamble, Nitin
AU  - Kamble N
AUID- ORCID: 0000-0003-1442-3293
AD  - The Pirbright Institute, Ash Road, Woking, United Kingdom.
FAU - Kaufer, Benedikt B
AU  - Kaufer BB
AUID- ORCID: 0000-0003-1328-2695
AD  - Institut fur Virologie, Freie Universitat Berlin, Robert-von-Ostertag-Strasse,
      Berlin, Germany.
FAU - Pathan, Ansar
AU  - Pathan A
AUID- ORCID: 0000-0002-5759-3420
AD  - College of Health and Life Sciences, Department of Life Sciences, Brunel
      University, London, United Kingdom.
FAU - Behboudi, Shahriar
AU  - Behboudi S
AUID- ORCID: 0000-0001-6455-4086
AD  - The Pirbright Institute, Ash Road, Woking, United Kingdom.
AD  - Department of Pathology and Infectious Disease, School of Veterinary Medicine,
      University of Surrey, Guildford, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171221
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cell Transformation, Viral/physiology
MH  - Chickens
MH  - Immune Tolerance/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Marek Disease/*immunology
MH  - T-Lymphocyte Subsets/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Transforming Growth Factor beta/immunology
PMC - PMC5739506
EDAT- 2017/12/22 06:00
MHDA- 2018/01/03 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/07/16 00:00 [received]
PHST- 2017/11/11 00:00 [accepted]
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
AID - 10.1371/journal.ppat.1006745 [doi]
AID - PPATHOGENS-D-17-01513 [pii]
PST - epublish
SO  - PLoS Pathog. 2017 Dec 21;13(12):e1006745. doi: 10.1371/journal.ppat.1006745.
      eCollection 2017 Dec.